Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,078,179 G→A 8.7% G301G (GGG→GGA)  PP_1853 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,078,1790GA 8.7% 128.1 / 2.0 46G301G (GGG→GGA) PP_1853LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (24/18);  new base A (2/2);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

GGCCGGGCGGCTGCTGCGGGTGTTGCCGGACTGGTATGTGGACGATGGGCATATCAGCCTGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTCCTTGTGGGAGCGGGCGGGCCCGCGAAGAGGCC  >  NC_002947/2078038‑2078326
                                                                                                                                              |                                                                                                                                                     
ggCCGGGCGGCTGCTGCGGGTGTTGCCGGACTGGTATGTGGACGATGGGCATATCAGCCTGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑ggcgg                                                                                                                                              >  2:268119/1‑149 (MQ=255)
  ccGGGCGGCTGCTGCGGGGGCTGCCGGACTGCTATGTTGACAATGGGCATATCAGCCTGGATTTCGCCTAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑ggcgggg                                                                                                                                            <  2:27811/149‑1 (MQ=255)
           tgctgcGGGTGTTGCCGGACTGCTATGTGGACGATGGGCATATCAGCCTGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGAt                                                                                                                                   >  7:227314/1‑149 (MQ=255)
             ctgcGGGTGTTGCCGGACTGGTATGTGGACGATGGGCATATCAGCCTGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATg                                                                                                                                  <  1:268119/148‑1 (MQ=255)
                cGGGTGTTGCCGGACTGGTATGTGGACGATGGGCATATCAGCCTGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑AATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGccc                                                                                                                               <  6:137470/148‑1 (MQ=255)
                     gtTGCCGGACTGGTATGTGGACGATGGGCATATCAGCCTGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAgg                                                                                                                         >  5:342765/1‑149 (MQ=255)
                            gACTGGTATGTGGACGATGGGCATATCAGCCTGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTa                                                                                                                  <  6:342765/149‑1 (MQ=255)
                                    tgtgGACGATGGGCATATCAGCCTGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGCTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAatt                                                                                                           <  7:162491/148‑1 (MQ=255)
                                              gggCATATCAGCCTGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGACTGTGc                                                                                                >  1:23547/1‑149 (MQ=255)
                                                gCATATCAGCCTGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCg                                                                                              >  5:229497/1‑149 (MQ=255)
                                                    atCAGCCTGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCt                                                                                          >  1:87168/1‑149 (MQ=255)
                                                     tCAGCCTGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCtt                                                                                         >  8:163677/1‑149 (MQ=255)
                                                       aGCCTGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCtttt                                                                                       >  7:126730/1‑149 (MQ=255)
                                                       aGCCTGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCtttt                                                                                       >  5:231466/1‑149 (MQ=255)
                                                           tGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTcgcg                                                                                   >  1:270704/1‑149 (MQ=255)
                                                             tATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTTGCATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGACT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCgg                                                                                  >  1:74936/1‑149 (MQ=255)
                                                              cactCGCCGCGCACAAGCTGTGGCCGCGCAAGACCCGGGCGTT‑CATCGCTCTTGTGGTGGAGCAGTTCGCCGAGCAGGGGCT‑‑GGCCGGACGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGgc                                                                                <  8:306759/146‑1 (MQ=255)
                                                               tttCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGgcc                                                                               >  2:192987/1‑149 (MQ=255)
                                                                ttCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGgccc                                                                              >  6:316548/1‑149 (MQ=255)
                                                                   gCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGgcccgcc                                                                           >  3:233053/1‑149 (MQ=255)
                                                                   gCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGACGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGgc                                                                                >  7:306759/1‑144 (MQ=255)
                                                                        gCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCAc                                                                      >  4:102557/1‑149 (MQ=255)
                                                                         cacaAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGACT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCAcc                                                                     >  7:146987/1‑149 (MQ=255)
                                                                            aaGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTCTTCGCGGGCCCGCCCGCTCCca                                                                   >  2:110959/1‑148 (MQ=255)
                                                                                  ttGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGat                                                            <  8:126730/149‑1 (MQ=255)
                                                                                  ttGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGa                                                             >  2:206579/1‑148 (MQ=255)
                                                                                    gCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCg                                                                                              >  4:76928/1‑113 (MQ=255)
                                                                                    gCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCg                                                                                              <  3:76928/113‑1 (MQ=255)
                                                                                     ccGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCTGGGGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGata                                                           >  1:103350/1‑149 (MQ=255)
                                                                                     ccGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGatat                                                          >  4:305356/1‑148 (MQ=255)
                                                                                     ccGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGatat                                                          <  2:87168/148‑1 (MQ=255)
                                                                                      cGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGACT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATca                                                        <  8:146987/149‑1 (MQ=255)
                                                                                        ggCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATcaca                                                      <  2:270704/149‑1 (MQ=255)
                                                                                         gCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAg                                                     <  3:305356/149‑1 (MQ=255)
                                                                                                       tt‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTg                                       <  7:163677/149‑1 (MQ=255)
                                                                                                           aTCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTg                                                                                                                             <  5:57475/60‑1 (MQ=255)
                                                                                                           aTCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTg                                                                                                                             >  6:57475/1‑60 (MQ=255)
                                                                                                                     tggtggAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTCCTTGTgg                           <  6:231466/148‑1 (MQ=255)
                                                                                                                       gtggAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTGCTTGTgggcg                        >  3:172090/1‑147 (MQ=255)
                                                                                                                              aGTTTGCCTAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACTGCCCTGATGACAGTGGGGTCCTTGTGGGAgcgggcgg                 <  8:227314/149‑1 (MQ=255)
                                                                                                                                  tGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAgg                                                               >  8:228752/1‑99 (MQ=255)
                                                                                                                                  tGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAgg                                                               <  7:228752/99‑1 (MQ=255)
                                                                                                                                    ccGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTCCTTGTGGGAGCGGGCGGGCCcgc           <  1:143304/149‑1 (MQ=255)
                                                                                                                                      gAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCca                                                                   >  3:134156/1‑91 (MQ=255)
                                                                                                                                      gAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCca                                                                   <  4:134156/91‑1 (MQ=255)
                                                                                                                                       aGCAGGGACT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGTATATCACAGCCCTGAAGACAGTGGGGTCCTTGTGGGAGCGGGCGGGCCCGCGaa        <  2:74936/149‑1 (MQ=255)
                                                                                                                                            gggCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACCGCCCTGAAGACAGTGGGGTCCGTGTGGGAGCGGGCGGGCCCGCGAAGAGGc   >  4:99564/1‑149 (MQ=255)
                                                                                                                                             ggCT‑‑GGCGGGGCGGTTCTATGCCCTGAAGGCCCTTTTGATATTTTTTGCCTGTGCCGGCTTTTTGGCGGGCCCGCCCGCACCCACTGGTATATCACTGCCGTTAAGAGCGTGGGGTCCTTGTGGGTGGGGGCGGGCCCGCGAAGAGGcc  <  2:23547/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                     
GGCCGGGCGGCTGCTGCGGGTGTTGCCGGACTGGTATGTGGACGATGGGCATATCAGCCTGTATTTCGCCGAGCACAAGCTGTTGCCGGGCAAGACCCGGGCGTT‑CATCGATTTTGTGGTGGAGCAGTTTGCCGAGCAGGGGCT‑‑GGCGGGGCGGTTCGATGCCCTGCAGGCCCTTTAGAGAATTATTGCCTGTGCCGGCCTTTTCGCGGGCCCGCCCGCACCCACAGGGATATCACAGCCCTGAAGACAGTGGGGTCCTTGTGGGAGCGGGCGGGCCCGCGAAGAGGCC  >  NC_002947/2078038‑2078326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑