Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,081,082	A→T	6.0%	*190Y (TAA→TAT)	efp →	elongation factor P

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,081,082	0	A	T	6.0%	202.0 / 2.8	67	*190Y (TAA→TAT)	efp	elongation factor P
Reads supporting (aligned to +/- strand):  ref base A (36/27);  new base T (2/2);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

TCGCGGTGACACCTCCGGCAAAGTCATGAAGCCTGCCAAGCTGGCGAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAGGGGATCGCGCAAGCCTTTGCGCCTTAGACGGTTACGTGCAGGCGCACATCCAC  >  NC_002947/2080938‑2081226                                                                                                                                                 |                                                                                                                                                 tCGCGGTGACACCTCCGGCAAAGTCATGAAGCCTGCCAAGCTGGCGAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGct                                                                                                                                              >  2:163853/1‑149 (MQ=255)   gcgGTGACACCTCCGGCAAAGTCATGAAGCCTGCCAAGCTGGCGAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGctt                                                                                                                                             <  7:324685/148‑1 (MQ=255)           acCTCCGGCAAAGTCATGAAGCCTGCCAAGCTGGCGAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCgaga                                                                                                                                    >  1:224468/1‑149 (MQ=255)            ccTCCGGCAAAGTCATGAAGCCTGCCAAGCTGGCGAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGCATTACTTCTTggagcgg                                                                                                                                   <  2:106245/149‑7 (MQ=255)             cTCCGGCAAAGTCATGAAGCCTGCCAAGCTGGCGAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACg                                                                                                                                  >  1:151432/1‑149 (MQ=255)             cTCCGGCAAAGTCATGAAGCCTGCCAAGCTGGCGAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACg                                                                                                                                  >  5:19283/1‑149 (MQ=255)              tCCGGCAAAGTCATGAAGCCTGCCAAGCTGGCGAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACg                                                                                                                                  >  6:51922/1‑148 (MQ=255)              tCCGGCAAAGTCATGAAGCCTGCCAAGCTGGCGAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACg                                                                                                                                  >  8:13216/1‑148 (MQ=255)                 ggCAAAGTCATGAAGCCTGCCAAGCTGGCGAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCaa                                                                                                                               >  2:273691/1‑148 (MQ=255)                            gAAGCCTGCCAAGCTGGCGAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGgcg                                                                                                                    >  2:126937/1‑148 (MQ=255)                              aGCATGCCAAGCTGGCGAACGGTACCGAGATCTCTGTTGCCGACTACATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCaa                                                                                                                 <  7:168808/149‑1 (MQ=255)                                  tGCCAAGCTGGCGAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGgc                                                                                                                     <  5:245530/141‑1 (MQ=255)                                  tGCCAAGCTGGCGAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGgc                                                                                                                     >  6:245530/1‑141 (MQ=255)                                   gCCAAGCTGGCGAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCgg                                                                                                            >  3:287661/1‑149 (MQ=255)                                      aaGCTGGCGAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCAACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGtt                                                                                                         >  4:350246/1‑149 (MQ=255)                                         cTGGCGAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGttttt                                                                                                      >  5:340051/1‑149 (MQ=255)                                         cTGGCGAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGttttt                                                                                                      <  4:130267/149‑1 (MQ=255)                                            gCGAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGtttttt                                                                                                     <  1:163853/147‑1 (MQ=255)                                              gAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGc                                                                                                 >  1:167717/1‑149 (MQ=255)                                              gAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGc                                                                                                 <  6:19283/149‑1 (MQ=255)                                                       cGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGCATTACTTCTTg                                                                                                                                         <  8:310352/100‑1 (MQ=255)                                                       cGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGCATTACTTCTTg                                                                                                                                         >  7:310352/1‑100 (MQ=255)                                                                ggTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATc                                                                                 <  1:273691/147‑1 (MQ=255)                                                                ggTTGCCGACTTCATCCAGATCCACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTGTTTGTGCCTGTGTTTTCCCAATCCt                                                                               >  4:328009/1‑149 (MQ=255)                                                                 gTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTGCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCAGGGTTTTTCTTGCCTTTCTTTTCCCAAtaat                                                                               >  6:250343/1‑145 (MQ=255)                                                                    gCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAgc                                                                           >  4:208395/1‑149 (MQ=255)                                                                     ccGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCaaaaaa                                                                                                                           <  7:17875/100‑1 (MQ=255)                                                                     ccGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCaaaaaa                                                                                                                           >  8:17875/1‑100 (MQ=255)                                                                     ccGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAgc                                                                           <  5:51922/148‑1 (MQ=255)                                                                      cGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAgcgc                                                                         >  5:91859/1‑149 (MQ=255)                                                                             aTCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCtt                                                                   <  1:126937/148‑1 (MQ=255)                                                                                cAGATCGAGGTGTGCATCGACATCGACACTCGCGACAACAGCTGCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAAGCCGGCGCTAGCCGGGTTTTTTGTGCCTGTGTTTTCCGAATCCTCAGCGCGTGCTtgtg                                                                <  6:24484/148‑1 (MQ=255)                                                                                 aGATCGACGAGTGGATCGACATCGACACTCGCGACCACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGtttttt                                                                                                     <  8:68830/110‑1 (MQ=255)                                                                                 aGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGtttttt                                                                                                     >  7:68830/1‑110 (MQ=255)                                                                                 aGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCt                                                              >  5:169551/1‑149 (MQ=255)                                                                                 aGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCt                                                              >  6:48393/1‑149 (MQ=255)                                                                                    tCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGCCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCCAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTcc                                                            >  1:254124/1‑148 (MQ=255)                                                                                       acgaGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTcc                                                            >  8:52336/1‑145 (MQ=255)                                                                                       acgaGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTcc                                                            <  7:52336/145‑1 (MQ=255)                                                                                       acgaGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCcaca                                                        <  4:287661/149‑1 (MQ=255)                                                                                       acgaGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCcaca                                                        <  2:167717/149‑1 (MQ=255)                                                                                           gTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCa                                                                             <  1:222575/124‑1 (MQ=255)                                                                                           gTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCa                                                                             >  2:222575/1‑124 (MQ=255)                                                                                           gTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAgggg                                                    <  7:13216/149‑1 (MQ=255)                                                                                           gTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAggg                                                     >  5:351225/1‑148 (MQ=255)                                                                                                    catcgacaCTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAGGGGATCGCGCaa                                           <  6:351225/149‑1 (MQ=255)                                                                                                    catcgacaCTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAGGGGATCGCGCaa                                           <  5:48393/149‑1 (MQ=255)                                                                                                     atcgacaCTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAGGGGATCGCGCAAg                                          >  7:77089/1‑149 (MQ=255)                                                                                                          cacTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCGGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAAt                                                                                  <  6:45865/104‑1 (MQ=255)                                                                                                          cacTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAAt                                                                                  >  5:45865/1‑104 (MQ=255)                                                                                                          cacTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAGGGGATCGCGCAAGCCttt                                     <  6:91859/149‑1 (MQ=255)                                                                                                             tCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAGGGGATCGCGCAAGCCTTTgcg                                  >  7:271500/1‑149 (MQ=255)                                                                                                               gcgACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAGGGGATCGCGCAAGCCTTTGCGcc                                <  6:169551/149‑1 (MQ=255)                                                                                                                   caacaGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTcc                                                            <  1:229882/117‑1 (MQ=255)                                                                                                                   caacaGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTcc                                                            >  2:229882/1‑117 (MQ=255)                                                                                                                    aacaGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAGGGGATCGCGCAAGCCTTTGCGCCTTAGa                           <  2:80707/149‑1 (MQ=255)                                                                                                                    aacaGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTCGTGCTCCCACAGGGGATCGCGCAAGCCTTTGCGCCTTAGa                           >  1:329823/1‑149 (MQ=255)                                                                                                                         cTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACGCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAGGGGATCGCGCAAGCCTTTGCGCCTTAGACGGtt                      <  3:328009/149‑1 (MQ=255)                                                                                                                          ttCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAGGGGATCGCGCAAGCCTTTGCGCCTTAGACGGTTa                     >  6:122878/1‑149 (MQ=255)                                                                                                                            cAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGtttttt                                                                                                     >  8:278538/1‑67 (MQ=255)                                                                                                                            cAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAGGGGATCGCGCAAGCCTTTGCGCCTTAGACGGTTAc                    <  8:271500/148‑1 (MQ=255)                                                                                                                            cAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAGGGGATCGCGCAAGCCTTTGCGCCTTAGACGGTTACg                   >  3:222104/1‑149 (MQ=255)                                                                                                                                   cGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAGGGGATCGCGCAAGCCTTTGCGCCTTAGACGGTTACGTGCAGgc            >  1:276609/1‑149 (MQ=255)                                                                                                                                     tcgcAAGAAGCATTACTTCTTG‑GAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAGGGGATCGCGCAAGCCTTTGCGCCTTAGACGGTTACGTGCAGGCGca         >  1:4060/4‑149 (MQ=255)                                                                                                                                        cAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAGGGGATCGCGCAAGCCTTTGCGCCTTAGACGGTTACGTGCAGGCGcaca       >  8:276086/1‑149 (MQ=255)                                                                                                                                        cAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAGGGGATCGCGCAAGCCTTTGCGCCTTAGACGGTTACGTGCAGGCGcac        >  8:55929/1‑148 (MQ=255)                                                                                                                                             agTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAGGGGATCGCGCAAGCCTTTGCGCCTTAGACGGTTACGTGCAGGCGCACATCcac  <  2:151432/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                 TCGCGGTGACACCTCCGGCAAAGTCATGAAGCCTGCCAAGCTGGCGAACGGTACCGAGATCTCGGTTGCCGACTTCATCCAGATCGACGAGTGGATCGACATCGACACTCGCGACAACAGCTTCAAAGGCCGCTCCAAGAAGTAATGCTTCTTGCGAGACGCAAAAAACCCGGCGCAAGCCGGGTTTTTTGTGCCTGTGTTTTCCCAATCCTCAGCGCGTGCTTGTGCTCCCACAGGGGATCGCGCAAGCCTTTGCGCCTTAGACGGTTACGTGCAGGCGCACATCCAC  >  NC_002947/2080938‑2081226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑