Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,146,710 A→T 8.0% Q213H (CAA→CAT)  murB → UDP‑N‑acetylenolpyruvoylglucosamine reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,146,7100AT 8.0% 139.6 / 3.4 50Q213H (CAA→CAT) murBUDP‑N‑acetylenolpyruvoylglucosamine reductase
Reads supporting (aligned to +/- strand):  ref base A (25/21);  new base T (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

GCTGCGTGACTTCAGTTTGGCGGAATGTGCTTTCGGCTATCGTGACAGCCTTTTCAAGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTGGGCAATGCCGGGAGCTTCTTCAAGAACCCGGTGGTAACGGCTGATCT  >  NC_002947/2146563‑2146856
                                                                                                                                                   |                                                                                                                                                  
gCTGCGTGACTTCAGTTTGGCGGAATGTGCTTTCGGCTATCGTGACAGCCTTTTCAAGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAAc                                                                                                                                                   <  3:137110/149‑1 (MQ=255)
 cTGCGTGACTTCAGTTTGGCGGAATGTGCTTTCGGCTATCGTGACAGCCTTTTCAAGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACg                                                                                                                                                  <  5:195094/149‑1 (MQ=255)
     gTGACTTCAGTTTGGCGGAATGTGCTTTCGGCTATCGTGACAGCCTTTTCAAGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTg                                                                                                                                              >  7:15943/1‑149 (MQ=255)
               tttGGCGGAATGTGCTTTCGGCTATCGTGACAGCCTTTTCAAGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCggg                                                                                                                                    <  8:15943/149‑1 (MQ=255)
                   gCGGAATGTGCTTTCGGCTATCGTGACAGCCTTTTCAAGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGgtgt                                                                                                                                >  4:123545/1‑149 (MQ=255)
                           tgCTTTCGGCTATCGTGACAGCCTTTTCAAGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAg                                                                                                                         <  6:129567/148‑1 (MQ=255)
                              tttCGGCTATCGTGACAGCCTTTTCAAGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGa                                                                                                                     >  7:145830/1‑149 (MQ=255)
                              tttCGGCTATCGTGACAGCCTTTTCAAGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGa                                                                                                                     >  2:329770/1‑149 (MQ=255)
                                       tCGTGACAGCCTTTTCAAGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAg                                                                                                            >  1:70427/1‑149 (MQ=255)
                                            aCAGCCTTTTCAAGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGAt                                                                                                        <  2:70427/148‑1 (MQ=255)
                                            aCAGCCTTTTCAAGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGAt                                                                                                        <  3:123545/148‑1 (MQ=255)
                                             gacccTTTTCAAGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCa                                                                                                      <  7:209833/146‑1 (MQ=255)
                                             cAGCCTTTTCAAGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGc                                                                                                                        >  6:113395/1‑131 (MQ=255)
                                             cAGCCTTTTCAAGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGc                                                                                                                        <  5:113395/131‑1 (MQ=255)
                                               gCCTTTTCAAGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGt                                                                                                    <  7:247912/149‑1 (MQ=255)
                                                  ttttCAAGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGAt                                                                                                 >  7:199496/1‑149 (MQ=255)
                                                       aaGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCgg                                                                                                                                     <  7:112627/108‑1 (MQ=255)
                                                       aaGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCgg                                                                                                                                     >  8:112627/1‑108 (MQ=255)
                                                         gcgcAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCt                                                                                          >  5:228687/1‑149 (MQ=255)
                                                             aaCCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGa                                                                                                  <  1:162810/137‑1 (MQ=255)
                                                             aaCCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGa                                                                                                  >  2:162810/1‑137 (MQ=255)
                                                                           gTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCgag                                                                         >  7:240949/1‑148 (MQ=255)
                                                                                        gtgCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATcc                                                           >  6:232661/1‑149 (MQ=255)
                                                                                                  cccTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTg                                                 <  1:329770/149‑1 (MQ=255)
                                                                                                   ccTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTgg                                                <  3:63882/149‑1 (MQ=255)
                                                                                                      gACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTGGGc                                              >  6:272882/1‑148 (MQ=255)
                                                                                                        cccGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTGGGCAAt                                           <  8:199496/149‑1 (MQ=255)
                                                                                                         ccGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTGGGCAATg                                          >  1:65186/1‑149 (MQ=255)
                                                                                                          cGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCATCGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGc                                                                  >  4:8034/1‑124 (MQ=255)
                                                                                                          cGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCATCGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGc                                                                  <  3:12928/124‑1 (MQ=255)
                                                                                                          cGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCATCGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGc                                                                  <  3:8034/124‑1 (MQ=255)
                                                                                                          cGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCATCGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGc                                                                  >  4:12928/1‑124 (MQ=255)
                                                                                                            cACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTGGGCAATGCCg                                       <  8:240949/149‑1 (MQ=255)
                                                                                                                 tGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCaga                                                     <  5:231463/130‑1 (MQ=255)
                                                                                                                 tGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCaga                                                     >  6:231463/1‑130 (MQ=255)
                                                                                                                      gCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTGGGCAATGCCGGGAGcttctt                             >  4:77521/1‑149 (MQ=255)
                                                                                                                        tCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTGGGCAATGCCgg                                      <  6:105633/138‑1 (MQ=255)
                                                                                                                        tCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTGGGCAATGCCgg                                      >  5:105633/1‑138 (MQ=255)
                                                                                                                          aTCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTGGGCAATGCCgg                                      >  8:62871/1‑136 (MQ=255)
                                                                                                                          aTCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTGGGCAATGCCgg                                      <  7:62871/136‑1 (MQ=255)
                                                                                                                            cTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGCTCCGGCAGCGTTGGGGAATGCCGGGAGCTTCTGCAAGaa                       >  5:367259/1‑149 (MQ=255)
                                                                                                                             tGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTGGGCAATGCCGGGAGCTTCTTCAAGAAc                      >  1:297540/1‑149 (MQ=255)
                                                                                                                                aTTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTGGGCAATGCCGGGAGCTTCTTCAAGAACCCg                   <  2:65186/149‑1 (MQ=255)
                                                                                                                                 ttACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGt                                                   <  2:84258/116‑1 (MQ=255)
                                                                                                                                 ttACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGt                                                   >  1:84258/1‑116 (MQ=255)
                                                                                                                                 ttACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTGGGCAATGCCGGGAGCTTCTTCAAGAACCCgg                  >  3:90228/1‑149 (MQ=255)
                                                                                                                                    cGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTGGGCAATGCCGGGAGCTTCTTCAAGAACCCggtg                <  4:90228/148‑1 (MQ=255)
                                                                                                                                     ggCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTCATCCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTGGGCAATGACGGGAGCTTCTTCAACAACCCcgtcg               >  8:297793/1‑146 (MQ=255)
                                                                                                                                                ccAACGCCTGTCTGAGCGGGGTGTGACCGAGATGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTGGGCAATGCCGGGAGCTTCTTCAAGAACCCGGTGGTAACGGCTGATc   >  5:94107/1‑149 (MQ=255)
                                                                                                                                                 cAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTGGGCAATGCCGGGAGCTTCTTCAAGAACCCGGTGGTAACGGCTGATCt  >  8:42267/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCTGCGTGACTTCAGTTTGGCGGAATGTGCTTTCGGCTATCGTGACAGCCTTTTCAAGCGCAACCCTGGCCGCTGGTTGATCCTGCGTGTGCGCTTTGCCCTGACCCGCACCCTGCACGCTCATCTGGATTACGGCCCGGTGCGCCAACGCCTGTCTGAGCGGGGTGTGACCGAGCTGACCGCGCAAGCGATCAGTGATGCAATCTGCAGCATTCGCCGCGAGAAGCTGCCAGATCCGGCAGAGTTGGGCAATGCCGGGAGCTTCTTCAAGAACCCGGTGGTAACGGCTGATCT  >  NC_002947/2146563‑2146856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑