Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,237,680 C→G 9.8% A302P (GCG→CCG) ‡ PP_1975 ← EmrB/QacA family drug resistance transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,237,6800CG 9.8% 143.6 / 5.0 49A302P (GCG→CCG) ‡PP_1975EmrB/QacA family drug resistance transporter
Reads supporting (aligned to +/- strand):  ref base C (21/23);  new base G (2/3);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

AAAACCCCAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAGGATGACGAAG  >  NC_002947/2237542‑2237825
                                                                                                                                          |                                                                                                                                                 
aaaaCCCCAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGGCGCAGGTACAgg                                                                                                                                         <  1:169128/149‑2 (MQ=255)
aaaaCCCCAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGc                                                                                                                                         <  4:259712/149‑1 (MQ=255)
     cccAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAg                                                                                                                                    >  5:211167/1‑149 (MQ=255)
       cAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCa                                                                                                                                  <  8:324451/149‑1 (MQ=255)
       cAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCa                                                                                                                                  >  5:69929/1‑149 (MQ=255)
         gcgcgcATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCaga                                                                                                                                <  1:235523/149‑1 (MQ=255)
                  gTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAg                                                                                                                       <  6:110246/149‑1 (MQ=255)
                   tACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCagata                                                                                                                              <  5:25956/141‑1 (MQ=255)
                   tACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCagata                                                                                                                              >  6:25956/1‑141 (MQ=255)
                     ccTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCa                                                                                                                                                 >  2:151306/1‑120 (MQ=255)
                     ccTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCa                                                                                                                                                 <  1:151306/120‑1 (MQ=255)
                      cTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCa                                                                                                                                  <  6:229624/134‑1 (MQ=255)
                      cTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCa                                                                                                                                  >  5:229624/1‑134 (MQ=255)
                         gggCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTa                                                                                                                  >  7:253275/1‑147 (MQ=255)
                                   aCCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGc                                                                                                                      >  5:69775/1‑133 (MQ=255)
                                   aCCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGc                                                                                                                      <  6:69775/133‑1 (MQ=255)
                                         atcaGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGGCGCAGGTACAggg                                                                                                                                        >  6:326448/1‑107 (MQ=255)
                                         atcaGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGGCGCAGGTACAggg                                                                                                                                        <  5:326448/109‑3 (MQ=255)
                                           caGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGcc                                                                                                >  8:331637/1‑147 (MQ=255)
                                                 cGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTg                                                                                         <  6:69929/148‑1 (MQ=255)
                                                       cAGCGGGATCAGGAACAGCTGCGGAATACCAATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGACGTACAGGCCCAAACCCATGCCCAGGCTGGCGATGc                                                                                  >  4:163797/1‑149 (MQ=255)
                                                             gATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCGGGCGATGCTCGACa                                                                            <  3:237995/149‑1 (MQ=255)
                                                              aTCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAAGCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCTCACCCATGCCCAGGCTGGCGATGCTCGACAg                                                                           <  3:163797/149‑1 (MQ=255)
                                                                  ggAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGc                                                                                       <  5:239469/133‑1 (MQ=255)
                                                                  ggAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGc                                                                                       >  6:239469/1‑133 (MQ=255)
                                                                       aGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGACCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGtt                                                                  >  7:123208/1‑149 (MQ=255)
                                                                                    ccATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAgg                                                                          <  4:37113/128‑1 (MQ=255)
                                                                                    ccATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAgg                                                                          >  3:37113/1‑128 (MQ=255)
                                                                                     cATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGa                                                    >  2:290224/1‑149 (MQ=255)
                                                                                     cATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGa                                                    <  6:211167/149‑1 (MQ=255)
                                                                                            aTGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAg                                             >  5:183855/1‑149 (MQ=255)
                                                                                                    cTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTg                                                                                         >  7:73737/1‑97 (MQ=255)
                                                                                                    cTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTg                                                                                         <  8:73737/97‑1 (MQ=255)
                                                                                                    cTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGAt                                       >  5:356188/1‑147 (MQ=255)
                                                                                                     tCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAg                                    >  3:121132/1‑149 (MQ=255)
                                                                                                       gCCAATCTGCAAGGCGTTGTAACCCTGTAGCTGCGGCAGGTACAGGGGTAGCAGATAGATAGAGCCGTACAGGCCCACACCCATGCCGAGGCTGGCGATGCTCGACAGGCGaaa                                                                     <  2:70544/114‑1 (MQ=255)
                                                                                                       gCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCg                                  <  4:121132/149‑1 (MQ=255)
                                                                                                       cccAATCTGCAAGGCGTTGTAACCCTGAACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACACATGCCCAGGATGCCGATGCTCCACAGGCCaaa                                                                     >  1:70544/2‑114 (MQ=255)
                                                                                                           aTCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTTATCAGCGGGTg                              <  8:123208/149‑1 (MQ=255)
                                                                                                             cTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCaaa                                                                     <  4:287240/108‑1 (MQ=255)
                                                                                                             cTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCaaa                                                                     >  3:287240/1‑108 (MQ=255)
                                                                                                               gCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGc                                                                                  <  6:71201/93‑1 (MQ=255)
                                                                                                                       ttGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGaaaa                  <  7:331637/149‑1 (MQ=255)
                                                                                                                            aCCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGc              <  6:356188/148‑1 (MQ=255)
                                                                                                                             cccTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAg            >  7:90158/1‑149 (MQ=255)
                                                                                                                               cTGTACCTGGCGCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAGGa          >  2:102242/1‑149 (MQ=255)
                                                                                                                               cTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTg                       >  2:305840/1‑136 (MQ=255)
                                                                                                                               cTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTg                       <  1:305840/136‑1 (MQ=255)
                                                                                                                                  tACCTGCTCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTAGAGGCCCACACACATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAGGATGa       <  8:253275/149‑1 (MQ=255)
                                                                                                                                       gcgcCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAGGATGACGAAg  >  4:21537/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                 
AAAACCCCAGCGCGCATAGTACCTTGGGCGATATCACCTTCATCAGTTGCGGCACCAGCGGGATCAGGAACAGCTGCGGAATACCCATCCACATGATCACCTCGCCAATCTGCAAGGCGTTGTAACCCTGTACCTGCGCCAGGTACAGCGGTAGCAGATAGATCGAGCCGTACAGGCCCACACCCATGCCCAGGCTGGCGATGCTCGACAGGCCAAAGTTGCGGTTGCCGAGGATACGCAGGTTGATCAGCGGGTGAGGCTTGGAAAACTGCAGGATGACGAAG  >  NC_002947/2237542‑2237825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑