Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,241,843	G→C	8.7%	noncoding (35/76 nt)	PP_t27 →	tRNA‑Ala

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,241,843	0	G	C	8.7%	176.1 / 6.0	57	noncoding (35/76 nt)	PP_t27	tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base G (27/25);  new base C (3/2);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

GGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTC  >  NC_002947/2241704‑2241987                                                                                                                                             |                                                                                                                                                 ggACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCagga                                                                                                                                         <  5:209450/149‑1 (MQ=255) ggACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCag                                                                                                                                           <  3:108184/147‑1 (MQ=255)    cacCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTATGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCATGCTGGGAGAGCGCCTGCATGCCATGCaggcgc                                                                                                                                       <  1:38026/149‑4 (MQ=38)    cacCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTgcatggca                                                                                                                                                <  5:186484/139‑1 (MQ=255)    cacCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTgcatggca                                                                                                                                                >  6:186484/1‑139 (MQ=255)     acCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGGTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GGTGGGAGCGCGCCTGCAGGGCATGCAGGAGGt                                                                                                                                      >  6:366708/1‑148 (MQ=255)     acCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGt                                                                                                                                      >  1:161208/1‑148 (MQ=255)     acCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGt                                                                                                                                      >  3:224848/1‑148 (MQ=255)        gCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGc                                                                                                                                  <  4:323134/149‑1 (MQ=255)            tttCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCaggag                                                                                                                                        >  3:55636/1‑139 (MQ=255)            tttCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCaggag                                                                                                                                        <  4:55636/139‑1 (MQ=255)            tttCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCagga                                                                                                                                         >  6:324518/1‑138 (MQ=255)            tttCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCagga                                                                                                                                         <  5:324518/138‑1 (MQ=255)            tttCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGtt                                                                                                                              >  2:44734/1‑149 (MQ=255)             ttCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTc                                                                                                                             >  7:100634/1‑149 (MQ=255)              tCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGtt                                                                                                                              <  6:255547/147‑1 (MQ=255)                accgcggTAACAGGCGGTTGCGTCCCCTAGGGCACGCCCCTTTTACACACCCGATCTTTAAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑CCTGGGAGAGCGCCTGCATGCCATGCAGGAGGGCAGCGGTTCGAt                                                                                                                          <  4:25414/146‑1 (MQ=255)                       tAACAGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCtt                                                                                                                   <  4:224848/149‑1 (MQ=255)                         aCAGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTa                                                                                                                  <  5:219083/148‑1 (MQ=255)                           aGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATccc                                                                                                                       <  5:266735/141‑1 (MQ=255)                           aGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATccc                                                                                                                       >  6:266735/1‑141 (MQ=255)                            ggggTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTc                                                                                                              >  2:150864/1‑149 (MQ=255)                              ggTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTcca                                                                                                            <  5:205779/149‑1 (MQ=255)                                   gAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAt                                                                                                       >  3:321869/1‑149 (MQ=255)                                      tCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAtttt                                                                                                    <  5:366708/149‑1 (MQ=255)                                         ccTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGcc                                                                                                 >  8:324277/1‑149 (MQ=255)                                         ccTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGcc                                                                                                 >  2:113150/1‑149 (MQ=255)                                              gggACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGt                                                                                            >  5:305775/1‑149 (MQ=255)                                                  cGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTcgc                                                                                        <  8:100634/149‑1 (MQ=255)                                                  cGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTcgc                                                                                        <  1:145476/149‑1 (MQ=255)                                                    ccAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGt                                                                                      >  2:240546/1‑149 (MQ=255)                                                                aaGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCgg                                                                           >  8:197031/1‑148 (MQ=255)                                                                aaGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCgg                                                                           >  2:19280/1‑148 (MQ=255)                                                                aaGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGc                                                                          <  1:150864/149‑1 (MQ=255)                                                                aaGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGc                                                                          >  6:349338/1‑149 (MQ=255)                                                                          ttAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGATTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGtt                                                                 <  1:240546/148‑1 (MQ=255)                                                                                tCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTccc                                                          >  4:246834/1‑149 (MQ=255)                                                                                        ccGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGc                                                                                  >  6:70029/1‑117 (MQ=255)                                                                                        ccGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGc                                                                                  <  5:70029/117‑1 (MQ=255)                                                                                         cgcgAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTa                                                 <  3:246834/149‑1 (MQ=255)                                                                                           cgAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGt                                               <  7:324277/149‑1 (MQ=255)                                                                                               gCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCAc                                                                        >  5:363697/1‑120 (MQ=255)                                                                                               gCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCAc                                                                        <  6:363697/120‑1 (MQ=255)                                                                                                  aGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAg                                        >  6:298874/1‑149 (MQ=255)                                                                                                   gAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTa                                         <  5:349338/147‑1 (MQ=255)                                                                                                       tCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGacac                                   <  2:161208/149‑1 (MQ=255)                                                                                                              cTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAg                                        <  2:3023/137‑1 (MQ=255)                                                                                                              cTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAg                                        >  1:3023/1‑137 (MQ=255)                                                                                                                 taGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCTATTAGCTCCACCAAATTTGCCGCGGTTCGCGTTAGCTGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTc                          <  1:113150/148‑1 (MQ=255)                                                                                                                  aGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCAc                        >  1:212860/1‑149 (MQ=255)                                                                                                                   gCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCAcg                       >  3:287116/1‑149 (MQ=255)                                                                                                                   gCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCAc                        <  1:44734/148‑1 (MQ=255)                                                                                                                    cTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCAcgg                      >  1:183902/1‑149 (MQ=255)                                                                                                                     tCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCAcggc                     >  1:61639/1‑149 (MQ=255)                                                                                                                           tGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGt                                               >  2:158325/1‑118 (MQ=255)                                                                                                                           tGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGt                                               <  1:158325/118‑1 (MQ=255)                                                                                                                              ctgacctccTGCATGCCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAg             >  8:57455/8‑149 (MQ=255)                                                                                                                                    tccTGCATGCCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTc       >  7:37738/2‑149 (MQ=255)                                                                                                                                    tccTGCATGCCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTc       >  3:78660/2‑149 (MQ=255)                                                                                                                                         catggcatgCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTc  >  6:161987/1‑149 (MQ=255)                                                                                                                                             |                                                                                                                                                 GGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAGTTTTACACAAGCGATGTTTTAATGTCGCTGGGCCGCGAGGCTAGAAATCCGGGGCTATAGCTCA‑GCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAATTTTGCCGCGGTTCGCGTTAGCGGATCGGCACGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTC  >  NC_002947/2241704‑2241987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑