Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 194,608 A→C 5.7% E38D (GAA→GAC) ‡ PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947194,6080AC 5.7% 219.3 / 4.0 70E38D (GAA→GAC) ‡PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base A (36/30);  new base C (2/2);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

AGGGAAGTACCCCATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCTTCGGTG  >  NC_002947/194460‑194755
                                                                                                                                                    |                                                                                                                                                   
aGGGAAGTACCCCATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGaa                                                                                                                                                     <  4:43293/149‑1 (MQ=255)
          cccATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGCACTTCCTCACCg                                                                                                                                           <  5:51348/149‑1 (MQ=255)
             aTCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCACCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTc                                                                                                                                        >  3:258982/1‑149 (MQ=255)
                gggTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCgg                                                                                                                                     <  6:317018/149‑1 (MQ=255)
                 ggTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAg                                                                                                                                                    <  7:102387/133‑1 (MQ=255)
                 ggTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAg                                                                                                                                                    >  8:102387/1‑133 (MQ=255)
                  gTCACACGGAGAGTCCAATGAGCAGCGTTGTGGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGt                                                                                                                                   >  5:285183/1‑149 (MQ=255)
                   tCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTg                                                                                                                                  >  6:333665/1‑149 (MQ=255)
                       acGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGgcg                                                                                                                               >  2:60543/1‑148 (MQ=255)
                       acGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTC‑AAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGgcg                                                                                                                               >  2:436/1‑147 (MQ=255)
                          gagaGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGt                                                                                                                           <  3:137182/149‑1 (MQ=255)
                               tCCAATGCGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCa                                                                                                                             <  7:164908/142‑1 (MQ=255)
                               tCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCa                                                                                                                             >  8:164908/1‑142 (MQ=255)
                               tCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACtt                                                                                                                      <  3:27054/149‑1 (MQ=255)
                                 cAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTg                                                                                                                    >  5:45900/1‑149 (MQ=255)
                                 cAATGAGCAGCGTTGTAGCCATCGTC‑AAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTgg                                                                                                                   <  1:436/149‑1 (MQ=255)
                                  aaTGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTg                                                                                                                    >  2:271681/1‑148 (MQ=255)
                                    tGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAg                                                                                                                 <  6:45900/149‑1 (MQ=255)
                                       gcagcGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAgctg                                                                                                              <  2:6018/149‑1 (MQ=255)
                                        cagcGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGCACTTCCTCACCg                                                                                                                                           <  3:179733/119‑1 (MQ=255)
                                        cagcGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGCACTTCCTCACCg                                                                                                                                           >  4:179733/1‑119 (MQ=255)
                                                tAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTg                                                                                                                    >  6:256517/1‑134 (MQ=255)
                                                tAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTg                                                                                                                    <  5:256517/134‑1 (MQ=255)
                                                        gTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCt                                                                                             <  6:285183/149‑1 (MQ=255)
                                                           aaaaGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGa                                                                                          >  5:35547/1‑149 (MQ=255)
                                                           aaaaGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGa                                                                                          >  3:218229/1‑149 (MQ=255)
                                                           aaaaGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGa                                                                                          >  8:219607/1‑149 (MQ=255)
                                                            aaaGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGAt                                                                                         >  5:80976/1‑149 (MQ=255)
                                                             aaGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATc                                                                                        <  7:219607/149‑1 (MQ=255)
                                                                     tCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGt                                                                                >  2:335352/1‑149 (MQ=255)
                                                                        gCCAGGTTATTGCGGTATCACCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACAGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGa                                                                              >  6:168106/1‑148 (MQ=255)
                                                                          cAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAg                                                                           <  4:218229/149‑1 (MQ=255)
                                                                             gTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACttt                                                                                                                     >  8:270102/1‑104 (MQ=255)
                                                                             gTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACttt                                                                                                                     <  7:270102/104‑1 (MQ=255)
                                                                                aTTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAAt                                                                      <  2:110829/148‑1 (MQ=255)
                                                                                 ttGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATg                                                                     <  2:323028/148‑1 (MQ=255)
                                                                                  tGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGc                                                                                              >  4:151407/1‑122 (MQ=255)
                                                                                  tGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGc                                                                                              <  3:151407/122‑1 (MQ=255)
                                                                                   gCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGa                                                                   <  5:333665/148‑1 (MQ=255)
                                                                                      gTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAgcgc                                                               >  6:149920/1‑149 (MQ=255)
                                                                                           cccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGAt                                                           <  1:60543/148‑1 (MQ=255)
                                                                                           cccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATg                                                          >  5:317735/1‑149 (MQ=255)
                                                                                           cccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATg                                                          >  3:46530/1‑149 (MQ=255)
                                                                                           cccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGCCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATg                                                          >  2:125733/1‑149 (MQ=255)
                                                                                            ccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGa                                                                                                                  <  7:77201/92‑1 (MQ=255)
                                                                                            ccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGa                                                                                                                  >  8:77201/1‑92 (MQ=255)
                                                                                            ccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAg                                                                  <  5:242895/140‑1 (MQ=255)
                                                                                            ccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAg                                                                  <  5:248129/140‑1 (MQ=255)
                                                                                            ccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAg                                                                  >  6:242895/1‑140 (MQ=255)
                                                                                            ccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAg                                                                  >  6:248129/1‑140 (MQ=255)
                                                                                              agagGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGATGGCCGGTGAGGAAGTGCTCACTGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGATGATGGCCGACTGCTAGATCTTGGACGTGACAGCCAATGGAGCGCCGATgcgc                                                       >  4:263458/1‑149 (MQ=255)
                                                                                                                aCTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGAcc                                     >  5:151993/1‑149 (MQ=255)
                                                                                                                  ttATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCt                                    >  4:273626/1‑148 (MQ=255)
                                                                                                                     tCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGAt                                                           >  8:225092/1‑122 (MQ=255)
                                                                                                                         aGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCTGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACTTGACAACCAATGGAGCGCCGATGCTCCTGACAGTAGCACCGACCTGAGCCAgg                            <  4:258982/149‑1 (MQ=255)
                                                                                                                              aCCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAggcggc                        <  1:271681/148‑1 (MQ=255)
                                                                                                                               ccGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAg                      <  3:273626/149‑1 (MQ=255)
                                                                                                                                cGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGc                     >  1:104097/1‑149 (MQ=255)
                                                                                                                                 gCCTGTTGGCCGGTGATGAAGTGATCACCGGTCCGGGTGGCGAAGTGACTTTGGCGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGAGCCGATGCGCCTGACAGTAGAACCGCCCTGAGCCAGGCGGCAGc                     >  6:342092/1‑148 (MQ=255)
                                                                                                                                 gCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGcc                    >  4:314552/1‑149 (MQ=255)
                                                                                                                                  ccTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGccc                   <  1:335352/149‑1 (MQ=255)
                                                                                                                                     gTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAgg                <  4:46530/149‑1 (MQ=255)
                                                                                                                                      ttGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGc               <  5:149920/149‑1 (MQ=255)
                                                                                                                                        ggCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCa                              <  5:233065/132‑1 (MQ=255)
                                                                                                                                        ggCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCa                              >  6:233065/1‑132 (MQ=255)
                                                                                                                                        gaccGGTGAGCACTTCCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCcg             >  6:343149/3‑149 (MQ=255)
                                                                                                                                           cGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGa                                                                                   >  6:166373/1‑76 (MQ=255)
                                                                                                                                           cGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGa                                                                                   <  5:166373/76‑1 (MQ=255)
                                                                                                                                           cGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCgcgc          >  6:348554/1‑149 (MQ=255)
                                                                                                                                           cGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCgcgc          >  2:265966/1‑149 (MQ=255)
                                                                                                                                                  gAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCTTCGGt   <  2:104097/149‑1 (MQ=255)
                                                                                                                                                   aaGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCTTCGGTg  <  1:265966/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGGGAAGTACCCCATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCTTCGGTG  >  NC_002947/194460‑194755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑