Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,322,627	G→T	5.1%	T391T (ACG→ACT)	PP_2042 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,322,627	0	G	T	5.1%	252.0 / 3.1	78	T391T (ACG→ACT)	PP_2042	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (36/38);  new base T (2/2);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

TTGCAGACTGTGCCGCTTTTCTGGGTTCCAGCGCAGGCCAGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCGGCCAGACAGCGCCAGTACGCTGTTCGACG  >  NC_002947/2322479‑2322775                                                                                                                                                     |                                                                                                                                                     ttGCAGACTGTGCCGCTTTTCTGGGTTCCAGCGCAGGCCAGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACg                                                                                                                                                      >  3:222354/1‑149 (MQ=255)    cAGACTGTGCCGCTTTTCTGGGTTCCAGCGCAGGCCAGTCGCTGGTGGGCGAGCTACGCCTGGGCACCTCGCAGTACCTGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGgc                                                                                                                                                    <  8:60237/148‑1 (MQ=255)            gCCGCTTTTCTGGGTTCCAGCGCAGGCCAGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAg                                                                                                                                             <  1:309328/147‑1 (MQ=255)             ccGCTTTTCTGGGTTCCAGCGCAGGCCAGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAgggg                                                                                                                                          >  2:183536/1‑149 (MQ=255)             ccGCTTTTCTGGGTTCCAGCGCAGGCCAGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAgggg                                                                                                                                          <  4:222354/149‑1 (MQ=255)                     cTGGGTTCCAGCGCAGGCCAGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGgagctgag                                                                                                                                  >  3:282547/1‑149 (MQ=255)                     cTGGGTTCCAGCGCAGGCCAGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGgagctga                                                                                                                                   <  8:239172/148‑1 (MQ=255)                       gggTTCCAGCGCAGGCCAGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAAGGGgagctgagc                                                                                                                                 >  4:274097/1‑148 (MQ=255)                             cAGCGCAGGCCAGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACTGCGCTGCAGGGGGAGCTGAGCTACCGGc                                                                                                                          >  3:318571/1‑149 (MQ=255)                             cAGCGCAGGCCAGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACTGCGCTGCAGGGGGAGCTGAGCTACCGGc                                                                                                                          >  3:323750/1‑149 (MQ=255)                               gcgcAGGCCAGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCg                                                                                                                        <  4:43251/149‑1 (MQ=255)                                 gcAGGCCAGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGaa                                                                                                                      <  8:96307/149‑1 (MQ=255)                                  cAGGCCAGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAAc                                                                                                                     <  2:350901/149‑1 (MQ=255)                                   aGGCCAGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACa                                                                                                                    >  7:219982/1‑149 (MQ=255)                                     gCCAGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATg                                                                                                                  >  5:170684/1‑149 (MQ=255)                                     gCCAGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATg                                                                                                                  >  2:188635/1‑149 (MQ=255)                                        aGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGccc                                                                                                               <  1:188635/149‑1 (MQ=255)                                        aGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGccc                                                                                                               <  7:248710/149‑1 (MQ=255)                                        aGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGccc                                                                                                               <  7:253473/149‑1 (MQ=255)                                            gCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGc                                                                                                           >  1:14419/1‑149 (MQ=255)                                                        gcTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCaa                                                                                                    >  6:153798/1‑144 (MQ=255)                                                        gcTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCaa                                                                                                    <  5:153798/144‑1 (MQ=255)                                                         cTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTAc                                                                                              <  1:183536/149‑1 (MQ=255)                                                            cGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAAGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTAcc                                                                                             <  3:274097/147‑1 (MQ=255)                                                                 gggCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATatca                                                                                      >  6:342242/1‑149 (MQ=255)                                                                  ggCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATatca                                                                                      <  5:342242/148‑1 (MQ=255)                                                                     aCCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCa                                                                                  <  1:141897/149‑1 (MQ=255)                                                                       cTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAg                                                                                                         <  6:264927/124‑1 (MQ=255)                                                                       cTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAg                                                                                                         >  5:264927/1‑124 (MQ=255)                                                                       cTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATatcat                                                                                     <  2:348929/144‑1 (MQ=255)                                                                       cTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATatcat                                                                                     >  1:348929/1‑144 (MQ=255)                                                                        tCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTc                                                                               >  6:298035/1‑149 (MQ=255)                                                                          gCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTc                                                                               <  5:298035/147‑1 (MQ=255)                                                                            aGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCgttg                                                                           <  7:250939/149‑1 (MQ=255)                                                                               aCCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCgtt                                                                            >  8:130712/1‑145 (MQ=255)                                                                               aCCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCgtt                                                                            <  7:130712/145‑1 (MQ=255)                                                                                ccGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGttgttg                                                                        >  8:198215/1‑148 (MQ=255)                                                                                  gggTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAAc                                                                     >  7:44864/1‑149 (MQ=255)                                                                                        aaTACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTAc                                                                                              <  2:199914/118‑1 (MQ=255)                                                                                        aaTACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTAc                                                                                              >  1:199914/1‑118 (MQ=255)                                                                                            ccccGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGcc                                                           >  5:86180/1‑149 (MQ=255)                                                                                            ccccGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGcc                                                           <  3:263924/149‑1 (MQ=255)                                                                                             cccGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTAc                                                                                              >  6:16945/1‑113 (MQ=255)                                                                                             cccGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTAc                                                                                              <  5:16945/113‑1 (MQ=255)                                                                                             cccGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCg                                                          >  8:262660/1‑149 (MQ=255)                                                                                               cGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACCTGCCCATGc                                                                                                           <  3:130230/98‑1 (MQ=255)                                                                                               cGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGc                                                                                                           >  4:130230/1‑98 (MQ=255)                                                                                               cGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCt                                                        >  7:121325/1‑149 (MQ=255)                                                                                                     cATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGttgttg                                                                        >  5:282899/1‑127 (MQ=255)                                                                                                     cATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGttgttg                                                                        <  6:282899/127‑1 (MQ=255)                                                                                                      aTTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCACAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCtt                                                 >  3:138898/1‑149 (MQ=255)                                                                                                           ccTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTgg                                            <  7:334889/149‑1 (MQ=255)                                                                                                            ctctACGTCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTggg                                           <  7:252571/149‑1 (MQ=255)                                                                                                               tACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGa                                        >  3:48679/1‑149 (MQ=255)                                                                                                                 cGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACg                                      <  8:121325/149‑1 (MQ=255)                                                                                                                  ggCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACgg                                     >  1:186139/1‑149 (MQ=255)                                                                                                                      tttCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGt                                          <  1:182002/140‑1 (MQ=255)                                                                                                                      tttCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGAAGGCCGCTCGCCCTTGCTGGGt                                          >  2:182002/1‑140 (MQ=255)                                                                                                                         cGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCg                               <  2:186139/148‑1 (MQ=255)                                                                                                                          gTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCgg                              <  8:219982/148‑1 (MQ=255)                                                                                                                          gTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCGGc                             >  4:350531/1‑149 (MQ=255)                                                                                                                           ttCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGCACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCGGc                             >  5:289562/1‑148 (MQ=255)                                                                                                                           ttCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCGGcc                            <  8:44864/149‑1 (MQ=255)                                                                                                                           ttCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCGGc                             >  1:172802/1‑148 (MQ=255)                                                                                                                            tctcCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTgg                                            <  3:123558/132‑1 (MQ=255)                                                                                                                            tctcCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTgg                                            >  4:123558/1‑132 (MQ=255)                                                                                                                             ctcCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCGGCCa                           <  6:276688/148‑1 (MQ=255)                                                                                                                                 accaccCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCGGCCAGACAg                      >  3:22320/1‑149 (MQ=255)                                                                                                                                 accaccCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCGGCCAGACAg                      >  3:27098/1‑149 (MQ=255)                                                                                                                                    accCTGCGCACCGGCACTGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCGGCCAGACAgcgc                   <  4:323750/149‑1 (MQ=255)                                                                                                                                    accCTGCGCACCGGCACTGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCGGCCAGACAgcgc                   <  4:318571/149‑1 (MQ=255)                                                                                                                                    accCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCGGCCAGACAgcgc                   <  3:350531/149‑1 (MQ=255)                                                                                                                                      ccTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCGGCCa                           >  1:190093/1‑139 (MQ=255)                                                                                                                                      ccTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCGGCCa                           <  2:190093/139‑1 (MQ=255)                                                                                                                                        tGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTgg                                            >  2:211676/1‑120 (MQ=255)                                                                                                                                        tGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTgg                                            <  1:211676/120‑1 (MQ=255)                                                                                                                                          cgcACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCGGCCAGACAGCGCCAGTa              <  5:64883/148‑1 (MQ=255)                                                                                                                                            cACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCCACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCGGCCAGACAGCGCCAGTACGc           >  7:102848/1‑149 (MQ=255)                                                                                                                                             aCCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCGGCCAGACAGCGCCAGTACGCt          >  7:261719/1‑149 (MQ=255)                                                                                                                                                     ggCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCGGCCAGACAGCGCCAGTACGCTGTTCGACg  <  2:14419/149‑1 (MQ=255)                                                                                                                                                     ggCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCGGCCAGACAGCGCCAGTACGCTGTTCGACg  >  2:331418/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     TTGCAGACTGTGCCGCTTTTCTGGGTTCCAGCGCAGGCCAGTCGCTGGTCGGCGCGCTACGCCTGGGCACCTCGCAGTACCGGGTGCAATACCCCGAGGACATTCGCCTCTACGGCCTTTCGTTCTCCACCACCCTGCGCACCGGCACGGCGCTGCAGGGGGAGCTGAGCTACCGGCCGAACATGCCCATGCAGCTCAACGGTACCGATATCATCCAGTCGTTGTTGAACGTCGATGGCCGCTCGCCCTTGCTGGGTGACGGCCTGCGGCCAGACAGCGCCAGTACGCTGTTCGACG  >  NC_002947/2322479‑2322775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑