Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,336,497	C→T	7.8%	intergenic (‑41/+39)	PP_2052 ← / ← PP_5500	bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,336,497	0	C	T	7.8%	180.1 / 4.1	64	intergenic (‑41/+39)	PP_2052/PP_5500	bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (29/30);  new base T (2/3);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.46e-01

GAGAAGCTTGCTTGAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAGGTGGG  >  NC_002947/2336351‑2336645                                                                                                                                                   |                                                                                                                                                      gagaAGCTTGCTTGAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCaa                                                                                                                                                     <  1:164854/149‑1 (MQ=255)        ttgcttgAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGc                                                                                                                                                >  1:50921/1‑147 (MQ=255)        ttgcttgAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGc                                                                                                                                                >  1:39022/1‑147 (MQ=255)          gcttgAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCga                                                                                                                                              <  8:76650/147‑1 (MQ=255)           cttgAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGTTGCAGGCgag‑at                                                                                                                                          <  5:310156/149‑2 (MQ=255)           cttgAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGTTGCAGGCgag‑a                                                                                                                                           <  5:192219/148‑1 (MQ=255)            ttgAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AAc                                                                                                                                         >  2:170262/1‑149 (MQ=255)            ttgAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AAc                                                                                                                                         >  6:80973/1‑149 (MQ=255)              gAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACg                                                                                                                                        <  8:137343/148‑1 (MQ=255)               aGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGgt                                                                                                                                      <  4:208189/149‑1 (MQ=255)               aGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGgt                                                                                                                                      <  5:172125/149‑1 (MQ=255)                 tgtCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGgtgt                                                                                                                                    <  7:8546/149‑1 (MQ=255)                 tgtCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGgtgt                                                                                                                                    <  2:39022/149‑1 (MQ=255)                    ccTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGgtgtgt                                                                                                                                  <  5:215740/148‑1 (MQ=255)                     cTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGgtgt                                                                                                                                    >  4:106452/1‑145 (MQ=255)                     cTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGgtgt                                                                                                                                    <  3:106452/145‑1 (MQ=255)                     cTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGa                                                                                                                                <  1:33229/149‑1 (MQ=255)                      tGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAgg                                                                                                                                                 <  2:123970/132‑1 (MQ=255)                      tGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACgg                                                                                                                                       >  4:56866/1‑141 (MQ=255)                      tGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACgg                                                                                                                                       <  3:56866/141‑1 (MQ=255)                        aaGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCTTGCAACAGGCGAG‑AACGGTGTGTGAGc                                                                                                                              <  1:15187/148‑1 (MQ=255)                          gcgcAGTCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGc                                                                                                                           <  2:296231/149‑1 (MQ=255)                            gcAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAg                                                                                                                         >  4:273970/1‑149 (MQ=255)                                gcgcTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTggg                                                                                                                     <  7:67184/149‑1 (MQ=255)                                 cgcTCGGTGTCTAAGAGGGTGCCCTCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTgggg                                                                                                                    <  7:170700/149‑1 (MQ=255)                                      ggTGTCGAAGAGGTTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCACCAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGt                                                                                                               >  4:120297/1‑149 (MQ=255)                                       gtgtCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGt                                                                                                               >  3:284986/1‑148 (MQ=255)                                       gtgtCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTc                                                                                                              <  1:170262/149‑1 (MQ=255)                                            gAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTc                                                                                                         <  2:50921/149‑1 (MQ=255)                                                agGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCgggg                                                                                                     <  3:273970/149‑1 (MQ=255)                                                 gggTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCgggg                                                                                                     <  1:235854/148‑1 (MQ=255)                                                  ggTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑ATCGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGc                                                                                                   >  2:343793/1‑149 (MQ=255)                                                   gTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGgtgtgt                                                                                                                                  <  5:212895/117‑1 (MQ=255)                                                   gTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGgtgtgt                                                                                                                                  >  6:212895/1‑117 (MQ=255)                                                      ccatccatATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGcaca                                                                                                <  5:11562/148‑1 (MQ=255)                                                      ccatccatATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGcaca                                                                                                >  6:11562/1‑148 (MQ=255)                                                        atccatATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGc                                                                                                                                                <  2:248291/99‑1 (MQ=255)                                                        atccatATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGc                                                                                                                                                >  1:248291/1‑99 (MQ=255)                                                        atccatATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTggggg                                                                                                                   <  4:243927/127‑1 (MQ=255)                                                        atccatATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTggggg                                                                                                                   >  3:243927/1‑127 (MQ=255)                                                         tccatATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAACCATTGGCTTTATCCCCTTGTCGTTGCGTCAGGAATCTCGCCTGCAACATGAGAG‑AACGGTGTGTGAGGTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGtt                                                                                            >  1:113386/1‑149 (MQ=255)                                                                 gAAGCTGGCGCTGTAATTCTGTTTACCGTGAACGAACCGCATTGGCTGTTGCCGCTTGGCGTTCCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGGGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAggg                                                                                    <  2:113386/149‑1 (MQ=255)                                                                 gAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAggg                                                                                    <  5:80973/149‑1 (MQ=255)                                                                        gcgcTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGc                                                                              <  4:284986/148‑1 (MQ=255)                                                                         cgcTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCGCACGTTc                                                                                           <  4:218990/134‑1 (MQ=255)                                                                         cgcTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCGCACGTTc                                                                                           >  3:218990/1‑134 (MQ=255)                                                                               aaaTCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGt                                                                      <  1:140617/149‑1 (MQ=255)                                                                                aaTCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGCAACGGTGTGTGAGCTGCAGTGGGGGCAGTCa                                                                                                             <  1:37285/110‑1 (MQ=255)                                                                                aaTCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCa                                                                                                             >  2:37285/1‑109 (MQ=255)                                                                                      tttACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGTCCAG‑AACGGTGTGTTAGCTGCAGTGGGGGCATTCAGGAAGGGCTCACACGTTGTACACGGGGACGGTCAGTAGTGTCg                                                                  >  3:106326/1‑146 (MQ=255)                                                                                                 agaaaAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAggcgg                                                    >  5:366687/1‑149 (MQ=255)                                                                                                    aaaGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAggcggggc                                                 >  1:127965/1‑149 (MQ=255)                                                                                                    aaaGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAggcggggc                                                 >  1:359832/1‑149 (MQ=255)                                                                                                          ttGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCg                                           >  3:188259/1‑149 (MQ=255)                                                                                                                 ttGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGAGAGGGGGACGGTCAGGAGTGACGCAGAGGTATGGCGGGGCGGAGCGGGGc                                       >  5:293621/1‑146 (MQ=255)                                                                                                                    ccccTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTg                                    >  2:35138/1‑146 (MQ=255)                                                                                                                           tCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGt                          >  3:161235/1‑149 (MQ=255)                                                                                                                                     accgttctcGCCTGTTGCAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGt                >  3:235819/6‑149 (MQ=255)                                                                                                                                       cgttctcGCCTGTTGCAGGCGAG‑ATCGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAggcgggg                                                  <  5:297424/110‑1 (MQ=255)                                                                                                                                       cgttctcGCCTGTTGCAGGCGAG‑ATCGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAggcgggg                                                  >  6:297424/4‑113 (MQ=255)                                                                                                                                               ccTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAgg      >  4:205068/1‑149 (MQ=255)                                                                                                                                                cTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAggt     >  3:120899/1‑149 (MQ=255)                                                                                                                                                cTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAggt     >  8:227572/1‑149 (MQ=255)                                                                                                                                                  gCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGCGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAggtgg   >  2:31181/1‑149 (MQ=255)                                                                                                                                                   cAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGCCGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAggtggg  >  2:115337/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                      GAGAAGCTTGCTTGAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAG‑AACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAGGTGGG  >  NC_002947/2336351‑2336645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑