Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,394,761	T→A	6.5%	D211V (GAT→GTT)	dacB ←	D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,394,761	0	T	A	6.5%	265.8 / 5.6	87	D211V (GAT→GTT)	dacB	D‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base T (43/38);  new base A (2/4);  total (45/42)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTCCAGATTGCGCGCACGGCACCGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGAAGCGCAGGGCCTTGAGGTTGACCAGCAGCGAGTCGG  >  NC_002947/2394615‑2394908                                                                                                                                                     |                                                                                                                                                    cgttcCAGATTGCGCGCACGGCACCGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCa                                                                                                                                                   >  3:164447/1‑149 (MQ=255)  gttcCAGATTGCGCGCACGGCACCGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCAc                                                                                                                                                  >  3:190269/1‑149 (MQ=255)     caacctcaaggccctgcgcttcgtggcgcgcaatgatggtggcaaggtcaccgtcgccgtcgagccaccgattgcccgcatccgcatcgacaaccaggtcaagccggtagcctctaagaaatGCACCGG‑GTTGTAGCGC‑ACATCACCgg                                                                                                                                               <  6:321059‑M1/27‑1 (MQ=255)      cAGATTGCGCGCACGGCACCGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGt                                                                                                                                              >  3:140167/1‑149 (MQ=255)      cAGATTGCGCGCACGGCACCGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGt                                                                                                                                              >  3:145460/1‑149 (MQ=255)       aGATTGCGCGCACGGCACCGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCCCCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCTCCGG‑GTTGTAGCGC‑ACATCACCGGTg                                                                                                                                             >  5:110637/1‑149 (MQ=255)        gATTGCGCGCACGGCACCGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTg                                                                                                                                             >  7:199178/1‑148 (MQ=255)          ttGCGCGCACGGCACCGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCAt                                                                                                                                          <  7:183038/149‑1 (MQ=255)           tGCGCGCACGGCACCGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCAtt                                                                                                                                         >  7:184028/1‑149 (MQ=255)            gcgcgcACGGCACCGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCAttt                                                                                                                                        >  3:140511/1‑149 (MQ=255)                gcACGGCACCGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTa                                                                                                                                    <  1:159008/149‑1 (MQ=255)                  aCGGCACCGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTaga                                                                                                                                  <  1:108937/149‑1 (MQ=255)                  aCGGCACCGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTaga                                                                                                                                  <  3:140913/149‑1 (MQ=255)                  aCGGCACCGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTaga                                                                                                                                  <  4:140167/149‑1 (MQ=255)                  aCGGCACCGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTaga                                                                                                                                  <  4:145460/149‑1 (MQ=255)                  aCGGCACCGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTa                                                                                                                                    >  2:155570/1‑147 (MQ=255)                  aCGGCACCGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTa                                                                                                                                    >  3:161024/1‑147 (MQ=255)                    ggCACCGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGCCCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAgg                                                                                                                                >  8:89353/1‑149 (MQ=255)                        ccGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTAc                                                                                                                            <  4:164447/149‑1 (MQ=255)                           gcggcgTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCgg                                                                                                                         <  4:190269/149‑1 (MQ=255)                              gcgTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCtt                                                                                                                      <  6:328578/149‑1 (MQ=255)                                     tGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTgg                                                                                                               <  8:178093/149‑1 (MQ=255)                                     tGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTgg                                                                                                               >  1:112911/1‑149 (MQ=255)                                     tGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTgg                                                                                                               >  1:162294/1‑149 (MQ=255)                                             tCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCgat                                                                                                       >  3:58263/1‑149 (MQ=255)                                               gAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCgatg                                                                                                      <  4:174875/148‑1 (MQ=255)                                               gAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACAACACCGGGGGATTGGTTAGGGGGAACCGGGTTGCCCTGGTTgggggggc                                                                                                     >  8:55532/1‑142 (MQ=255)                                                       aCAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGatgcggatgcgg                                                                                             >  5:259698/1‑149 (MQ=255)                                                       aCAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGatgcggatgcgg                                                                                             <  7:24720/149‑1 (MQ=255)                                                       aCAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCATCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGatgcggatgcgg                                                                                             <  1:125080/149‑1 (MQ=255)                                                           gtaggtTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTg                                                                                                            <  6:75566/130‑1 (MQ=255)                                                           gtaggtTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTg                                                                                                            >  5:75566/1‑130 (MQ=255)                                                           gtaggtTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCgat                                                                                                       <  8:47080/135‑1 (MQ=255)                                                           gtaggtTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCgat                                                                                                       >  7:47080/1‑135 (MQ=255)                                                            taggtTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAAt                                                                                        <  8:184028/149‑1 (MQ=255)                                                            taggtTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGAAGCGGGCAAt                                                                                        >  1:293739/1‑149 (MQ=255)                                                               gtTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCgg                                                                                     >  2:267733/1‑149 (MQ=255)                                                                tttGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGt                                                                                    >  5:71435/1‑149 (MQ=255)                                                                     gAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTc                                                                               >  3:233644/1‑149 (MQ=255)                                                                          gCAGCCGTCACCTAGTTGGCCGCTGACCGTTTCACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTcgacgg                                                                          >  1:37354/1‑149 (MQ=255)                                                                           cAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTcgacggc                                                                         >  1:313250/1‑149 (MQ=255)                                                                           cAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGCTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTcgacggc                                                                         >  2:295677/1‑149 (MQ=255)                                                                           cAGCCGGCACCTAGTTGGCCGCGGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTcgacgg                                                                          <  4:102616/148‑1 (MQ=255)                                                                            agacGTCACCTAGTTGGCCGCTGACCGTTACCCTGATGCCGTCGGCCTGGGGCACCGG‑GGTGTAGCGC‑ACATCACCGGTGAATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGCTGGCTagacggag                                                                        >  4:234169/4‑147 (MQ=255)                                                                             gCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTcgacggcga                                                                       <  1:155570/149‑1 (MQ=255)                                                                               catCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTcgacggcgacg                                                                     <  6:71435/147‑1 (MQ=255)                                                                               cGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTcgacggcgacg                                                                     <  1:295677/149‑1 (MQ=255)                                                                                   aCCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTg                                                                  <  4:161024/148‑1 (MQ=255)                                                                                   aCCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAAACGGTGGCTCGACGGCGACGGTg                                                                  <  7:55532/148‑1 (MQ=255)                                                                                    ccTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGAc                                                                <  2:112911/149‑1 (MQ=255)                                                                                     cTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGAcc                                                               >  8:220240/1‑149 (MQ=255)                                                                                     cTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGAcc                                                               >  6:110742/1‑149 (MQ=255)                                                                                     cTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGAcc                                                               <  4:118649/149‑1 (MQ=255)                                                                                         ttGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGc                                                           >  5:248561/1‑149 (MQ=255)                                                                                         ttGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGc                                                           <  2:162294/149‑1 (MQ=255)                                                                                              cGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGccacca                                                      <  3:173645/149‑1 (MQ=255)                                                                                                   aCCGTTTCACTGTTTGCGTCGGGCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCTGGCAATCGGTGGCTCGACGGCGACGGt                                                                   <  3:73556/131‑1 (MQ=255)                                                                                                   aCCGTTACACTGATGCCGTCGGCCTGGGGCATCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCAtt                                                 <  3:278883/149‑1 (MQ=255)                                                                                                   aCCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACcatca                                                   <  8:199178/147‑1 (MQ=255)                                                                                                   aCCGTTACACTGATGCCGTCGGCCTGGGCCACCGG‑GTTTTAGCGC‑ACATCACCGGTGAATTTCTTAGAGGATACCGGCTTGACAGGGTTGTCGATGCGGATGCGGGCAAACGGTGCCTCGACGGCGAGGGt                                                                   >  4:73556/1‑131 (MQ=255)                                                                                                       ttACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTgcgc                                             >  6:69660/1‑149 (MQ=255)                                                                                                       tgacacTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTgcgc                                             <  4:140511/147‑1 (MQ=255)                                                                                                           acTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCACGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCa                                         >  1:310844/1‑149 (MQ=255)                                                                                                               aTGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACg                                       <  2:313250/147‑1 (MQ=255)                                                                                                               aTGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACg                                       >  6:131657/1‑147 (MQ=255)                                                                                                               aTGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGCGACCTTGCCACCATCATTGCGCGCCACGaa                                     <  2:37354/149‑1 (MQ=255)                                                                                                                tGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACTGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGAAg                                    <  2:293739/149‑1 (MQ=255)                                                                                                                tGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCATAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGTTGACCATGACAACATCAATGCGCGCCAcc                                       >  6:252796/1‑145 (MQ=255)                                                                                                                    gTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGAAgcg                                  >  4:118911/1‑147 (MQ=255)                                                                                                                    gTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGAAGCGCa                                >  4:48110/1‑149 (MQ=255)                                                                                                                    ctctaagaaatGCACCGGTGATGT‑GCGCTACAAC‑CCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGAAgcg                                  <  5:321059‑M1/136‑1 (MQ=255)                                                                                                                     tCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGAAGCGCAg                               <  4:233644/149‑1 (MQ=255)                                                                                                                      ctaagaaatGCACCGGTGATGT‑GCGCTACAAC‑CCGGTGCATTTCTTTGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGccacc                                                       >  4:269979‑M1/10‑124 (MQ=255)                                                                                                                      ctaagaaatGCACCGGTGATGT‑GCGCTACAAC‑CCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGccacc                                                       <  3:269979‑M1/115‑1 (MQ=255)                                                                                                                      ctaagaaatGCACCGGTGATGT‑GCGCTACAAC‑CCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGACGCGCAgg                              <  6:43612‑M1/140‑1 (MQ=255)                                                                                                                        aagaaatGCACCGGTGATGT‑GCGCTACAAC‑CCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGAAGCGCAGGGc                            <  8:135701‑M1/142‑1 (MQ=255)                                                                                                                             gggCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGAAGCGCAGGGCCtt                         <  5:131657/147‑1 (MQ=255)                                                                                                                              ggCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGAAGCGCAGGGCCTTGAg                      >  5:128228/1‑149 (MQ=255)                                                                                                                              ggCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGAAGCGCAGGGCCTTGAg                      <  8:52295/149‑1 (MQ=255)                                                                                                                                CACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGAAGCGCAGGGCCTTGAGGt                    >  6:248561‑M1/1‑149 (MQ=255)                                                                                                                                 ACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGAAGCGCAGGGCCTTGAGGtt                   >  1:267733‑M1/1‑149 (MQ=255)                                                                                                                                 ACCGG‑GGTGTAGCGC‑ACATCACCGTTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGGCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGAAGCGCAGGGCCTTGATGtt                   >  3:234169‑M1/1‑149 (MQ=255)                                                                                                                                     G‑GTTGTAGCGC‑ACATCACCGGTGCAGTTCTTAGAGGCTACCCGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGAAGCGCAGGGCCTTGAGGTTGAcc               >  8:206143‑M1/1‑149 (MQ=255)                                                                                                                                           tAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGAAGCGCAGGGCCTTGAGGTTGACcagcag          >  7:319940/1‑149 (MQ=255)                                                                                                                                                    aTCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGAAGCGCAg                               <  5:38710/120‑1 (MQ=255)                                                                                                                                                    aTCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGAAGCGCAg                               >  6:38710/1‑120 (MQ=255)                                                                                                                                                    aTCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGAGGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGAAGCGCAGGGCCTTGAGGTTGACCAGCAGCGAGTCgg  >  4:110923/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CGTTCCAGATTGCGCGCACGGCACCGGCGGCGTAGGTGGAATGGTCGAGCAGTGACAGGTAGGTTTGCGAGTTGCAGCCGTCACCTAGTTGGCCGCTGACCGTTACACTGATGCCGTCGGCCTGGGGCACCGG‑GTTGTAGCGC‑ACATCACCGGTGCATTTCTTAGAGGCTACCGGCTTGACCTGGTTGTCGATGCGGATGCGGGCAATCGGTGGCTCGACGGCGACGGTGACCTTGCCACCATCATTGCGCGCCACGAAGCGCAGGGCCTTGAGGTTGACCAGCAGCGAGTCGG  >  NC_002947/2394615‑2394908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑