Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 194,611 G→C 5.5% V39V (GTG→GTC) ‡ PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947194,6110GC 5.5% 235.5 / 2.9 72V39V (GTG→GTC) ‡PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (36/32);  new base C (2/2);  total (38/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

CCCATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCTTCGGTGGAA  >  NC_002947/194470‑194758
                                                                                                                                             |                                                                                                                                                   
cccATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGCACTTCCTCACCg                                                                                                                                              <  5:51348/149‑1 (MQ=255)
   aTCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCACCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTc                                                                                                                                           >  3:258982/1‑149 (MQ=255)
      gggTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCgg                                                                                                                                        <  6:317018/149‑1 (MQ=255)
        gTCACACGGAGAGTCCAATGAGCAGCGTTGTGGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGt                                                                                                                                      >  5:285183/1‑149 (MQ=255)
         tCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTg                                                                                                                                     >  6:333665/1‑149 (MQ=255)
             acGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGgcg                                                                                                                                  >  2:60543/1‑148 (MQ=255)
             acGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTC‑AAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGgcg                                                                                                                                  >  2:436/1‑147 (MQ=255)
                gagaGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGt                                                                                                                              <  3:137182/149‑1 (MQ=255)
                     tCCAATGCGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCa                                                                                                                                <  7:164908/142‑1 (MQ=255)
                     tCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCa                                                                                                                                >  8:164908/1‑142 (MQ=255)
                     tCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACtt                                                                                                                         <  3:27054/149‑1 (MQ=255)
                       cAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTg                                                                                                                       >  5:45900/1‑149 (MQ=255)
                       cAATGAGCAGCGTTGTAGCCATCGTC‑AAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTgg                                                                                                                      <  1:436/149‑1 (MQ=255)
                        aaTGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTg                                                                                                                       >  2:271681/1‑148 (MQ=255)
                          tGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAg                                                                                                                    <  6:45900/149‑1 (MQ=255)
                             gcagcGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAgctg                                                                                                                 <  2:6018/149‑1 (MQ=255)
                              cagcGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGCACTTCCTCACCg                                                                                                                                              >  4:179733/1‑119 (MQ=255)
                              cagcGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGCACTTCCTCACCg                                                                                                                                              <  3:179733/119‑1 (MQ=255)
                                      tAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTg                                                                                                                       <  5:256517/134‑1 (MQ=255)
                                      tAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTg                                                                                                                       >  6:256517/1‑134 (MQ=255)
                                              gTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCt                                                                                                <  6:285183/149‑1 (MQ=255)
                                                 aaaaGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGa                                                                                             >  8:219607/1‑149 (MQ=255)
                                                 aaaaGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGa                                                                                             >  5:35547/1‑149 (MQ=255)
                                                 aaaaGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGa                                                                                             >  3:218229/1‑149 (MQ=255)
                                                  aaaGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGAt                                                                                            >  5:80976/1‑149 (MQ=255)
                                                   aaGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATc                                                                                           <  7:219607/149‑1 (MQ=255)
                                                           tCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGt                                                                                   >  2:335352/1‑149 (MQ=255)
                                                              gCCAGGTTATTGCGGTATCACCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACAGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGa                                                                                 >  6:168106/1‑148 (MQ=255)
                                                                cAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAg                                                                              <  4:218229/149‑1 (MQ=255)
                                                                   gTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACttt                                                                                                                        >  8:270102/1‑104 (MQ=255)
                                                                   gTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACttt                                                                                                                        <  7:270102/104‑1 (MQ=255)
                                                                      aTTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAAt                                                                         <  2:110829/148‑1 (MQ=255)
                                                                       ttGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATg                                                                        <  2:323028/148‑1 (MQ=255)
                                                                        tGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGc                                                                                                 >  4:151407/1‑122 (MQ=255)
                                                                        tGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGc                                                                                                 <  3:151407/122‑1 (MQ=255)
                                                                         gCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGa                                                                      <  5:333665/148‑1 (MQ=255)
                                                                            gTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAgcgc                                                                  >  6:149920/1‑149 (MQ=255)
                                                                                 cccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGAt                                                              <  1:60543/148‑1 (MQ=255)
                                                                                 cccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATg                                                             >  3:46530/1‑149 (MQ=255)
                                                                                 cccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATg                                                             >  5:317735/1‑149 (MQ=255)
                                                                                 cccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGCCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATg                                                             >  2:125733/1‑149 (MQ=255)
                                                                                  ccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGa                                                                                                                     <  7:77201/92‑1 (MQ=255)
                                                                                  ccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGa                                                                                                                     >  8:77201/1‑92 (MQ=255)
                                                                                  ccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAg                                                                     <  5:242895/140‑1 (MQ=255)
                                                                                  ccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAg                                                                     <  5:248129/140‑1 (MQ=255)
                                                                                  ccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAg                                                                     >  6:248129/1‑140 (MQ=255)
                                                                                  ccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAg                                                                     >  6:242895/1‑140 (MQ=255)
                                                                                    agagGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGATGGCCGGTGAGGAAGTGCTCACTGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGATGATGGCCGACTGCTAGATCTTGGACGTGACAGCCAATGGAGCGCCGATgcgc                                                          >  4:263458/1‑149 (MQ=255)
                                                                                                      aCTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGAcc                                        >  5:151993/1‑149 (MQ=255)
                                                                                                        ttATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCt                                       >  4:273626/1‑148 (MQ=255)
                                                                                                           tCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGAt                                                              >  8:225092/1‑122 (MQ=255)
                                                                                                               aGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCTGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACTTGACAACCAATGGAGCGCCGATGCTCCTGACAGTAGCACCGACCTGAGCCAgg                               <  4:258982/149‑1 (MQ=255)
                                                                                                                    aCCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAggcggc                           <  1:271681/148‑1 (MQ=255)
                                                                                                                     ccGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAg                         <  3:273626/149‑1 (MQ=255)
                                                                                                                      cGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGc                        >  1:104097/1‑149 (MQ=255)
                                                                                                                       gCCTGTTGGCCGGTGATGAAGTGATCACCGGTCCGGGTGGCGAAGTGACTTTGGCGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGAGCCGATGCGCCTGACAGTAGAACCGCCCTGAGCCAGGCGGCAGc                        >  6:342092/1‑148 (MQ=255)
                                                                                                                       gCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGcc                       >  4:314552/1‑149 (MQ=255)
                                                                                                                        ccTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGccc                      <  1:335352/149‑1 (MQ=255)
                                                                                                                           gTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAgg                   <  4:46530/149‑1 (MQ=255)
                                                                                                                            ttGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGc                  <  5:149920/149‑1 (MQ=255)
                                                                                                                              ggCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCa                                 >  6:233065/1‑132 (MQ=255)
                                                                                                                              ggCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCa                                 <  5:233065/132‑1 (MQ=255)
                                                                                                                              gaccGGTGAGCACTTCCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCcg                >  6:343149/3‑149 (MQ=255)
                                                                                                                                 cGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGa                                                                                      >  6:166373/1‑76 (MQ=255)
                                                                                                                                 cGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGa                                                                                      <  5:166373/76‑1 (MQ=255)
                                                                                                                                 cGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCgcgc             >  6:348554/1‑149 (MQ=255)
                                                                                                                                 cGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCgcgc             >  2:265966/1‑149 (MQ=255)
                                                                                                                                        gAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCTTCGGt      <  2:104097/149‑1 (MQ=255)
                                                                                                                                         aaGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCTTCGGTg     <  1:265966/149‑1 (MQ=255)
                                                                                                                                            tGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCTTCGGTgg    >  5:62456/1‑147 (MQ=255)
                                                                                                                                            tGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCTTCGGTGGaa  <  5:168106/149‑1 (MQ=255)
                                                                                                                                            tGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGCCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACTGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCTTCGGTGGaa  <  1:125733/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
CCCATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCTTCGGTGGAA  >  NC_002947/194470‑194758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑