Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,423,086	T→G	7.1%	S364R (AGC→CGC)	PP_2124 ←	glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,423,086	0	T	G	7.1%	242.9 / 4.8	79	S364R (AGC→CGC)	PP_2124	glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (32/41);  new base G (3/3);  total (35/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGGCCCAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGGTGGTGATCACGGGTAAGCCGGCGGCCATCGCT  >  NC_002947/2422939‑2423234                                                                                                                                                    |                                                                                                                                                      aGCGGCCCAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCt                                                                                                                                                       >  3:112810/1‑148 (MQ=255)   cGGCCCAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCAt                                                                                                                                                    <  1:341761/149‑1 (MQ=255)    ggCCCAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATg                                                                                                                                                   >  6:105802/1‑149 (MQ=255)       ccagccagGCACTGGCCAGCGGCGCGGCCTCGACCGCTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGATGTTGGGCAGCCACGCTCCAGTCGTGCCGCTGGCGGGCAAACTCGCGCCCGGCATCGCTCAACTGGCTCATgcga                                                                                                                                                >  2:112157/1‑148 (MQ=255)             agGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGc                                                                                                                                          >  7:321259/1‑149 (MQ=255)              ggCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCt                                                                                                                                         <  2:125437/149‑1 (MQ=255)                 aCTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGAt                                                                                                                                      <  1:81886/149‑1 (MQ=255)                   tGGCCAGCGGCGCGGCCTCGTCCGGGGCCGCCTGGGGCAGGCGTATGTGCCCATTCTCCAGTTGTTGGGCCGCCACGCTCCAGTCGTGGCGCTGGCGGGCAACCTGGCGCCCGGCCTCGCGCAACTGGCGCATGCGCCAGGGCGGATTg                                                                                                                                    >  5:119207/1‑149 (MQ=255)                     gCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATgc                                                                                                                                                  >  1:136025/1‑133 (MQ=255)                     gCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATgc                                                                                                                                                  <  2:136025/133‑1 (MQ=255)                     gCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGag                                                                                                                                  <  5:120337/149‑1 (MQ=255)                     gCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGag                                                                                                                                  <  5:105802/149‑1 (MQ=255)                         gcggcgcggcCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGagcagc                                                                                                                              >  8:67117/1‑149 (MQ=255)                            gcgcggcCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTgg                                                                                                                           <  4:49573/149‑1 (MQ=255)                            gcgcggcCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTgg                                                                                                                           <  8:249583/149‑1 (MQ=255)                                   cTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGa                                                                                                                                   >  5:43712/1‑134 (MQ=255)                                   cTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGa                                                                                                                                   <  6:43712/134‑1 (MQ=255)                                   cTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCa                                                                                                                   <  4:141300/149‑1 (MQ=255)                                    tCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAg                                                                                                                  >  7:274359/1‑149 (MQ=255)                                       tCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCaa                                                                                                               <  5:4553/149‑1 (MQ=255)                                          ggTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGc                                                                                                            <  3:34099/149‑1 (MQ=255)                                                gccTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTg                                                                                                      <  6:57827/149‑1 (MQ=255)                                                     ggTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCa                                                                                                  <  7:52793/148‑1 (MQ=255)                                                      gTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATg                                                                                                             <  3:109072/136‑1 (MQ=255)                                                      gTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATg                                                                                                             >  4:109072/1‑136 (MQ=255)                                                      gTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCAcc                                                                                                <  6:315512/149‑1 (MQ=255)                                                       tCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACcg                                                                                               <  1:285085/149‑1 (MQ=255)                                                       ccAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACcg                                                                                               <  3:299303/148‑1 (MQ=255)                                                         aGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCTGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAAt                                                                                                              <  5:120704/132‑1 (MQ=255)                                                         aGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAAt                                                                                                              >  6:120704/1‑132 (MQ=255)                                                                 gtgCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGc                                                                                     >  4:249962/1‑149 (MQ=255)                                                                  tgCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGcc                                                                                    <  7:295119/149‑1 (MQ=255)                                                                  tgCACATTCGCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCGGGCGGGCAACCTGGCGCCCGGCAGCGCTCAACTGGCGCATGCGCCAGGGCTGATTGAGCCGCGGGGG‑GAGCAGCAATGCCAGTTGATCACCGGCGTCACTGcc                                                                                    <  6:119207/149‑1 (MQ=255)                                                                      caTTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGTT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAag                                                                                <  3:97793/149‑1 (MQ=255)                                                                          ctcCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAagtag                                                                             <  5:234904/148‑1 (MQ=255)                                                                           tcCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACcgtc                                                                                             <  3:269032/131‑1 (MQ=255)                                                                           tcCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACcgtc                                                                                             <  3:273825/131‑1 (MQ=255)                                                                           tcCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACcgtc                                                                                             >  4:269032/1‑131 (MQ=255)                                                                           tcCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACcgtc                                                                                             >  4:273825/1‑131 (MQ=255)                                                                           tcCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCa                                                                                         <  3:23946/135‑1 (MQ=255)                                                                           tcCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCa                                                                                         >  4:23946/1‑135 (MQ=255)                                                                           tcCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCAAGCGCCAGGGCTGAt                                                                                                                                      <  7:186939/91‑1 (MQ=255)                                                                           tcCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCAAGCGCCAGGGCTGAt                                                                                                                                      >  8:186939/1‑91 (MQ=255)                                                                                  gttgGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCg                                                                    >  3:157217/1‑149 (MQ=255)                                                                                            cACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGt                                                                                                        <  8:17984/103‑1 (MQ=255)                                                                                            cACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGt                                                                                                        >  7:17984/1‑103 (MQ=255)                                                                                            cACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACgg                                                          <  1:192755/149‑1 (MQ=255)                                                                                            cACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACgg                                                          <  2:86961/149‑1 (MQ=255)                                                                                             aCGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACgg                                                          >  4:223068/1‑148 (MQ=255)                                                                                               gCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCTGTTGTTCACGGt                                                         >  2:9428/1‑147 (MQ=255)                                                                                                   cAGTCGTGGCGCTGTCGGGCAAACTGGCGCCCGGAATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGc                                                    <  7:241157/148‑1 (MQ=255)                                                                                                    aGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCt                                                  <  8:274359/149‑1 (MQ=255)                                                                                                        gTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGt                                                         >  1:271787/1‑138 (MQ=255)                                                                                                        gTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGt                                                         <  2:271787/138‑1 (MQ=255)                                                                                                            cgcTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGcc                                          <  8:321259/149‑1 (MQ=255)                                                                                                                  cGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGAAGCTGGGT‑GATCAGCAATGACAGTTGATCACCGGCGTCACGGCCCAAGTAGTGTTCGCCGTTGTTAACGCTAAGGCCTGAAACGCCCTAGTc                                    >  4:71548/1‑149 (MQ=255)                                                                                                                    ggCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCgg                                  <  4:112810/149‑1 (MQ=255)                                                                                                                     gCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCggt                                 <  4:225278/149‑1 (MQ=255)                                                                                                                      cAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCggtg                                <  3:223068/149‑1 (MQ=255)                                                                                                                      cAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCggtg                                >  6:66676/1‑149 (MQ=255)                                                                                                                       aaaCTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCggtgg                               <  7:67117/149‑1 (MQ=255)                                                                                                                         aCTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCggtg                                >  6:26594/1‑146 (MQ=255)                                                                                                                         aCTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCggtg                                <  5:26594/146‑1 (MQ=255)                                                                                                                               gcCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGCTCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGGTGGTGATCACg                       >  7:137030/1‑149 (MQ=255)                                                                                                                                cccGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGGTGGTGATCACgg                      <  5:66676/149‑1 (MQ=255)                                                                                                                                 ccGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGTGGATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGGTGGTGATCACgg                      >  1:89488/1‑149 (MQ=255)                                                                                                                                 ccGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGGTGGTGATCACggg                     >  4:310724/1‑149 (MQ=255)                                                                                                                                      aTCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACGGCCGAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGGTGGTGCTCACGGGTAAGc                >  2:72771/1‑149 (MQ=255)                                                                                                                                         tcagcccTGGCGCATGAGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCtt                                       >  2:62805/7‑123 (MQ=255)                                                                                                                                         tcagcccTGGCGCATGAGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCtt                                       <  1:62805/117‑1 (MQ=255)                                                                                                                                         tcagcccTGGCGCATGAGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGgtggtg                             >  7:120523/7‑133 (MQ=255)                                                                                                                                         tcagcccTGGCGCATGAGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGgtggtg                             <  8:120523/127‑1 (MQ=255)                                                                                                                                         gCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGGTGGTGATCACGGGTAAGCcgg             >  5:140397/1‑149 (MQ=255)                                                                                                                                         gCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGGTGGTGATCACGGGTAAGCcgg             >  5:325300/1‑149 (MQ=255)                                                                                                                                         gCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGGTGGTGATCACGGGTAAGCcgg             >  3:342676/1‑149 (MQ=255)                                                                                                                                         gCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGGTGGTGATCACGGGTAAGCcgg             >  3:116688/1‑149 (MQ=255)                                                                                                                                           tCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGGTGGTGATCACGGGTAAGCcggcg           >  2:338906/1‑149 (MQ=255)                                                                                                                                             aaCTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCTGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGGTGGTGATCACGGGTAAGCcggcgg          <  1:9428/148‑1 (MQ=255)                                                                                                                                               cTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGGTGGTGATCACGGGTAAGCCGGCGGCCa       <  1:338906/149‑1 (MQ=255)                                                                                                                                                tGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGGTGGTGATCACGGGTAAGCCGGCGGCCAt      >  2:124703/1‑149 (MQ=255)                                                                                                                                                    tCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGGTGGTGATCACGGGTAAg                 <  8:101637/134‑1 (MQ=255)                                                                                                                                                    tCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGGTGGTGATCACGGGTAAg                 >  7:101637/1‑134 (MQ=255)                                                                                                                                                    tCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGGTGGTGATCACGGGTAAGCCGGCGGCCATCGCt  >  8:248757/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                      AGCGGCCCAGCCAGGCACTGGCCAGCGGCGCGGCCTCGTCCGGTGCCGCCTGGGTCAGGCGTATGTGCACATTCTCCAGTTGTTGGGCAGCCACGCTCCAGTCGTGGCGCTGGCGGGCAAACTGGCGCCCGGCATCGCTCAACTGGCTCATGCGCCAGGGCTGATTGAGCAGCTGGGT‑GATCAGCAATGCCAGTTGATCACCGTCGTCACTGCCCAAGTAGTGTTCGCCGTTGTTCACGGTCAGGCCTGAAACGCCCTTGTCGGTGGTGATCACGGGTAAGCCGGCGGCCATCGCT  >  NC_002947/2422939‑2423234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑