Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,467,904 C→T 5.4% P265L (CCG→CTG)  queF → NADPH‑dependent 7‑cyano‑7‑deazaguanine reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,467,9040CT 5.4% 278.5 / 2.2 91P265L (CCG→CTG) queFNADPH‑dependent 7‑cyano‑7‑deazaguanine reductase
Reads supporting (aligned to +/- strand):  ref base C (41/45);  new base T (2/3);  total (43/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCCAGCATGCCGACTTCCATGAGCAGTGTGTGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGGGTGGTCGGCCTCGAAGCGCT  >  NC_002947/2467758‑2468052
                                                                                                                                                  |                                                                                                                                                    
ctgCCAGCATGCCGACTTCCATGAGCAGTGTGTGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGcc                                                                                                                                                      <  5:181118/145‑1 (MQ=255)
 cGCCAGCATGCCGACTTCCATGAGCAGTGTGTGGAGCGGATTTACCTGCACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGa                                                                                                                                                    <  8:122022/148‑1 (MQ=255)
      gCATCCCGACTTCCATGAGCAGGGCGTGGAGCGCATTTACCTGGACTTGAACAATCTGCTGCACCCGGAGCACCTGACGCTCTATCCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTAGCGCAGCACCGGGCCGATCAGcc                                                                                                                                              <  6:95191/149‑1 (MQ=255)
        aTGCCGACTTCCATGAGCAGTGTGTGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCg                                                                                                                                            <  8:129130/149‑1 (MQ=255)
        aTGCCGACTTCCATGAGCAGTGTGTGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCg                                                                                                                                            <  3:49100/149‑1 (MQ=255)
         tGCCGACTTCCATGAGCAGTGGGTGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACTGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCgg                                                                                                                                           <  3:177536/149‑1 (MQ=255)
           ccGACTTCCATGAGCAGTGTGTGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGa                                                                                                                                          <  1:247992/148‑1 (MQ=255)
             gACTTCCATGAGCAGTGTGTGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCTGATCGGCCCggtgct                                                                                                                                       <  7:334285/149‑5 (MQ=255)
              aCTTCCATGAGCAGTGTGTGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAAc                                                                                                                                      <  4:262909/149‑1 (MQ=255)
              aCTTCCATGAGCAGTGTGTGGAGCGGATTTACCTGGACTTGAAGAATCCGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAAc                                                                                                                                      <  1:291287/149‑1 (MQ=255)
                   cATGAGCAGTGTGTGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAAc                                                                                                                                  <  6:7151/148‑1 (MQ=255)
                     tGAGCAGTGTGTGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGc                                                                                                                               <  7:222520/149‑1 (MQ=255)
                              tgtgGAGCGGATGTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCgg                                                                                                                       <  8:24447/148‑1 (MQ=255)
                                 ggtgCGTATTTACCTGGACTTGATGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGAGGCGCGTTGGGCTGGACATCAACCCGTATCGCTGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAg                                                                                                                    <  7:248286/145‑1 (MQ=255)
                                 ggAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGAt                                                                                                                                         >  8:41970/1‑127 (MQ=255)
                                 ggAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGAt                                                                                                                                         <  7:41970/127‑1 (MQ=255)
                                 ggAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAg                                                                                                                    >  1:273493/1‑148 (MQ=255)
                                  gAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGAt                                                                                                                                                   <  6:362248/116‑1 (MQ=255)
                                  gAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGt                                                                                                                   >  5:306804/1‑148 (MQ=255)
                                    gCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAAt                                                                                                                <  7:258123/149‑1 (MQ=255)
                                       gATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATa                                                                                                               >  7:76429/1‑147 (MQ=255)
                                            aCCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATAc                                                                                                              <  2:3256/143‑1 (MQ=255)
                                            aCCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATAc                                                                                                              >  1:3256/1‑143 (MQ=255)
                                             ccTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGAt                                                                                                                                         >  2:307885/1‑115 (MQ=255)
                                              cTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAAc                                                                                                                                      <  7:179661/117‑1 (MQ=255)
                                              cTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAAc                                                                                                                                      >  8:179661/1‑117 (MQ=255)
                                                ggACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGc                                                                                                                      <  2:248025/131‑1 (MQ=255)
                                                ggACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGc                                                                                                                      >  1:248025/1‑131 (MQ=255)
                                                ggACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGc                                                                                                    >  3:37732/1‑149 (MQ=255)
                                                 gACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCACCCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCa                                                                                                                     >  5:157231/1‑131 (MQ=255)
                                                 gACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCACCCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCa                                                                                                                     <  6:157231/131‑1 (MQ=255)
                                                   cTTGAAGAATCTGCTGCAGCCGGAGCACCTGCCGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCgg                                                                                                                       <  5:369294/127‑1 (MQ=255)
                                                   cTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCgg                                                                                                                       >  6:369294/1‑127 (MQ=255)
                                                    ttGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAACCGGCTGGTCCGGCAGTAATACCGGGGGCCGCTTTg                                                                                                >  5:188747/1‑149 (MQ=255)
                                                    ttGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTg                                                                                                >  6:323213/1‑149 (MQ=255)
                                                      gaagaaTCTGCTGCAGCCGGAGCACCTGACGGTCTATGTGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCg                                                                                              >  3:339735/1‑149 (MQ=255)
                                                      gaagaaTCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCACCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCg                                                                                              >  1:89830/1‑149 (MQ=255)
                                                       aagaaTCTGCTGCAGCCGGAGCACCTGACGGTCTATGTGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCg                                                                                              <  4:339735/148‑1 (MQ=255)
                                                         gaaTCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGcc                                                                                           >  1:113916/1‑149 (MQ=255)
                                                            tCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGccccc                                                                                        >  7:115000/1‑149 (MQ=255)
                                                                ctgcAGCCGTCGCCCCTGCCGGTCAATGCGCGCTATGTGCGCGGCGGTGGGCTGGACATCAACCCGTATCGCACCACCGGGCCGATCAGCCCGGATAAAAAACGGCTGGTCCGGCATTAATACAGGGGCCCGCTTTGCGGCCCCCGCTa                                                                                    <  6:202711/149‑1 (MQ=255)
                                                                     gCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCa                                                                               <  2:113916/149‑1 (MQ=255)
                                                                      ccGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAg                                                                              <  7:75887/149‑1 (MQ=255)
                                                                      ccGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAg                                                                              <  5:323213/149‑1 (MQ=255)
                                                                         gAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATc                                                                           >  3:45104/1‑149 (MQ=255)
                                                                             aCCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCa                                                                       <  4:45104/149‑1 (MQ=255)
                                                                              ccTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCAt                                                                      >  6:6768/1‑149 (MQ=255)
                                                                                 gACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCt                                                                   >  1:10891/1‑149 (MQ=255)
                                                                                 gACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCt                                                                   >  8:263028/1‑149 (MQ=255)
                                                                                  aCGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTa                                                                                                                  <  2:211435/101‑1 (MQ=255)
                                                                                  aCGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTa                                                                                                                  >  1:211435/1‑101 (MQ=255)
                                                                                  aCGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCAt                                                                      <  5:135839/145‑1 (MQ=255)
                                                                                  aCGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCAt                                                                      >  6:135839/1‑145 (MQ=255)
                                                                                     gTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCGCCGCTATTTCAGATCCCCATGCtgtg                                                                >  3:212106/1‑148 (MQ=255)
                                                                                     gTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCtgtg                                                                >  2:17469/1‑148 (MQ=255)
                                                                                      tCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCt                                                                   <  2:134075/144‑1 (MQ=255)
                                                                                      tCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCt                                                                   >  1:134075/1‑144 (MQ=255)
                                                                                      tCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCa                                                              >  2:244870/1‑149 (MQ=255)
                                                                                           gcgcgcTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGa                                                          <  8:76429/148‑1 (MQ=255)
                                                                                           gcgcgcTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAg                                                         >  8:164803/1‑149 (MQ=255)
                                                                                             gcgcTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGtt                                                       >  8:316748/1‑149 (MQ=255)
                                                                                               gcttttggcggcgCGGTGGGCTGGTAATGAAACCGTATTGGATGACCGGTGCGTAGAGGCCGGTTTAAAAAAGGCTTGTCCGGGATTAATACCCGGGGCCGGTTTGGGGGCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTgg                                                     <  4:43286/142‑1 (MQ=255)
                                                                                               gcTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTgg                                                     <  4:37732/149‑1 (MQ=255)
                                                                                                   tgtgCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCa                                                   <  5:6768/147‑1 (MQ=255)
                                                                                                       cgccgcGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAAtgctg                                             <  7:263028/149‑1 (MQ=255)
                                                                                                       cgccgcGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAAtgct                                              <  6:306804/148‑1 (MQ=255)
                                                                                                        gccgcGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAAtgctgc                                            >  1:38332/1‑149 (MQ=255)
                                                                                                            cgGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGc                                                    >  6:63678/1‑137 (MQ=255)
                                                                                                            cgGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGc                                                    <  5:63678/137‑1 (MQ=255)
                                                                                                             ggTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAgg                                       <  1:17469/149‑1 (MQ=255)
                                                                                                                gggCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTg                                    <  8:115000/149‑1 (MQ=255)
                                                                                                                     ggACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAACCGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGt                               <  6:160592/149‑1 (MQ=255)
                                                                                                                      gACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTg                              <  4:329171/149‑1 (MQ=255)
                                                                                                                       aCATCACCCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGt                               >  2:223588/1‑147 (MQ=255)
                                                                                                                           cAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTc                         >  4:128937/1‑149 (MQ=255)
                                                                                                                             aCCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCgg                       >  6:304872/1‑149 (MQ=255)
                                                                                                                             aCCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCgg                       <  3:128937/149‑1 (MQ=255)
                                                                                                                              cccGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCggg                      <  7:316748/149‑1 (MQ=255)
                                                                                                                               ccGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAg                                                             <  2:210356/109‑1 (MQ=255)
                                                                                                                               ccGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCgggg                     <  7:83263/149‑1 (MQ=255)
                                                                                                                               ccGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCgggg                     >  8:83263/1‑149 (MQ=255)
                                                                                                                               ccGTATCGCAGCACCGGGCCGATCAGCCCCGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAg                                                             >  1:210356/1‑109 (MQ=255)
                                                                                                                                cGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGggt                    >  7:334617/1‑149 (MQ=255)
                                                                                                                                    tCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGGGTGGTc                >  8:120002/1‑149 (MQ=255)
                                                                                                                                      gcagcaCCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGGGTGGTCgg              >  4:108518/1‑149 (MQ=255)
                                                                                                                                         gcaCCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGGGTGGTCGGCCt           <  6:322548/149‑1 (MQ=255)
                                                                                                                                             cGGGCTGATCGGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGGGTGGTCGGCCTCg         <  6:53642/147‑1 (MQ=255)
                                                                                                                                             cGGGCTGATCGGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGGGTGGTCGGCCTCg         <  6:48276/147‑1 (MQ=255)
                                                                                                                                             cGGGCTGATCGGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGGGTGGTCGGCCTCg         >  5:53642/1‑147 (MQ=255)
                                                                                                                                             cGGGCTGATCGGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGGGTGGTCGGCCTCg         >  5:48276/1‑147 (MQ=255)
                                                                                                                                               ggCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGCGGGCCGCTTTGCGGCCCCCGCTATTTCAGAACCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGGGTGGTCGGCCTCGAAgc     >  4:352192/1‑149 (MQ=255)
                                                                                                                                                gCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGGGTGGTCGGCCTCGAAgcg    >  2:85695/1‑149 (MQ=255)
                                                                                                                                                  cGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGGGTGGTc                <  6:318368/135‑1 (MQ=255)
                                                                                                                                                  cGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGGGTGGTc                >  5:318368/1‑135 (MQ=255)
                                                                                                                                                  cGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGGGTGGTCGGCCTCGAAGCGCt  <  7:164803/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CCGCCAGCATGCCGACTTCCATGAGCAGTGTGTGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCCGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCAGCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGGGTGGTCGGCCTCGAAGCGCT  >  NC_002947/2467758‑2468052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑