Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,482,504 T→A 6.8% intergenic (‑129/‑49) PP_2176 ← / → PP_2177 transcriptional regulator, LysR family/transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,482,5040TA 6.8% 253.5 / 7.3 88intergenic (‑129/‑49)PP_2176/PP_2177transcriptional regulator, LysR family/transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (39/43);  new base A (3/3);  total (42/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

GCTGGAGGATTCAGCCACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTGGCCGAGCGCATCGA  >  NC_002947/2482358‑2482650
                                                                                                                                                  |                                                                                                                                                  
gCTGGAGGATTCAGCCACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTa                                                                                                                                                   >  4:301109/1‑148 (MQ=255)
   ggaggaTTCAGCCACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAAtt                                                                                                                                               >  1:38060/1‑149 (MQ=255)
    gaggaTTCAGCCACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAAtt                                                                                                                                               <  2:60655/148‑1 (MQ=255)
     aggaTTCAGCCACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAAtttt                                                                                                                                             >  1:42043/1‑149 (MQ=255)
     aggaTTCAGCCACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAAttt                                                                                                                                              >  8:283461/1‑148 (MQ=255)
       gaTTCAGCCACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCa                                                                                                                                           >  3:257176/1‑149 (MQ=255)
            aGCCACGAGGATACTCGCCGAGAGCCTGTAAAGAAACCCGCGCCACCGCAGCAAATCCGCCACGTTTCGAAGCAAGCCCTATGTGGGCGGCTGCAAATTTCCATCGTTAGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGaa                                                                                                                                       >  6:336818/1‑148 (MQ=255)
               cACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAAt                                                                                                                                   <  3:301109/149‑1 (MQ=255)
               cACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAAt                                                                                                                                   <  2:90303/149‑1 (MQ=255)
                 ctaGGCTACTCGCCGATCGCCTGTAAAGAAACCAGAGCCACCTCAGCCAATCCGCCACGTTGCGCAGCAATCCCTCTGTTGGCGGCTGCACATTTCCATCGTTCGGCTTGACTCGTGAAATTTTTCATGTAAATTTTCATGAAAAATaa                                                                                                                                 <  8:126445/147‑1 (MQ=255)
                 cGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATaa                                                                                                                                 <  5:256070/149‑1 (MQ=255)
                 cGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGCTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATaa                                                                                                                                 <  5:69379/149‑1 (MQ=255)
                   aGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGt                                                                                                                               <  4:257176/149‑1 (MQ=255)
                   aGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGAAAATTTACATGAAAAAtttca                                                                                                                               <  3:235663/149‑5 (MQ=255)
                        aCTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGAAAATTTACATGAAAAAtttcacg                                                                                                                             <  5:110280/146‑7 (MQ=255)
                        aCTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGAAAATTTACATGAAAAAtttcacg                                                                                                                             >  6:110280/1‑140 (MQ=255)
                          tCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTaaaaa                                                                                                                        >  4:193249/1‑149 (MQ=255)
                            gCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGATATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAAtt                                                                                                                      >  5:311370/1‑149 (MQ=255)
                            gCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTc                                                                                                                                            <  1:175380/127‑1 (MQ=255)
                            gCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTc                                                                                                                                            >  2:175380/1‑127 (MQ=255)
                            gCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCAt                                                                                                                                          <  4:205467/129‑1 (MQ=255)
                            gCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCAt                                                                                                                                          >  3:205467/1‑129 (MQ=255)
                                 gcgcCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCGTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACg                                                                                                                 <  6:11547/149‑1 (MQ=255)
                                  cgcCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACgg                                                                                                                <  2:217163/149‑1 (MQ=255)
                                  cgcCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACgg                                                                                                                <  7:116157/149‑1 (MQ=255)
                                   gcCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACgg                                                                                                                >  8:23998/1‑148 (MQ=255)
                                            gAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGaaa                                                                                                      <  6:311370/149‑1 (MQ=255)
                                            gAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGaa                                                                                                       >  8:289759/1‑148 (MQ=255)
                                               aCCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGa                                                                                                   <  2:38060/149‑1 (MQ=255)
                                               aCCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGa                                                                                                   <  7:331618/149‑1 (MQ=255)
                                                cccGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGAt                                                                                                  <  7:178812/149‑1 (MQ=255)
                                                 ccGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGAt                                                                                                  >  6:59897/1‑148 (MQ=255)
                                                 ccGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATg                                                                                                 <  3:193249/149‑1 (MQ=255)
                                                   gcgcCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATg                                                                                                 >  4:140268/1‑147 (MQ=255)
                                                        aCCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGc                                                                                          <  7:193773/149‑1 (MQ=255)
                                                          cGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCAc                                                                                        <  5:59897/149‑1 (MQ=255)
                                                              gCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAag                                                                                    <  7:23998/149‑1 (MQ=255)
                                                                cAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAagag                                                                                  >  2:280026/1‑149 (MQ=255)
                                                                cAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTAATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAagag                                                                                  >  6:117514/1‑149 (MQ=255)
                                                                 aaTCCGCCACGTTGCGCAGCAAGCCCTCTGTGTGCTGCTGCACATTTCCATCGTTAGGGTTTACGCGTGAAATTTTTCATGTAAATTTTCATTAAAAATAAGTCTAAAAATTTCACTGCAGTCAGAAACGATGAAGATGCACGAAGAgg                                                                                 <  5:336818/149‑1 (MQ=255)
                                                                 aaTCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCa                                                                                         >  4:347223/1‑141 (MQ=255)
                                                                 aaTCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCa                                                                                         <  3:347223/141‑1 (MQ=255)
                                                                 aaTCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAgg                                                                                 >  4:345473/1‑149 (MQ=255)
                                                                 aaTCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAgg                                                                                 >  1:202813/1‑149 (MQ=255)
                                                                   tCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGtt                                                                               >  2:50340/1‑149 (MQ=255)
                                                                     cGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTg                                                                              >  1:12413/1‑148 (MQ=255)
                                                                      gCCACGTTGCGCATCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGaa                                                                            <  7:199267/149‑1 (MQ=255)
                                                                      gCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTg                                                                              >  5:91387/1‑147 (MQ=255)
                                                                      gCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGaa                                                                            <  6:185060/149‑1 (MQ=255)
                                                                      gCCACGTTGCGCAGCAAGCCCTCTGGTGGCTGCTGCACATTTCCTTCGTTCTGCTTTACGAGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCTCGATTAGGTTTaa                                                                            <  8:84124/149‑1 (MQ=255)
                                                                       ccACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAg                                                                           <  7:289759/149‑1 (MQ=255)
                                                                        cACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTAATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGaa                                                                            <  5:117514/147‑1 (MQ=255)
                                                                              gcgcAGCAAGCCCTCTCTGGTCGTCTGCACATTTCCATCGTTCGGCTAGACGCCTGAATTTTTTCAAGTAAATTATCATGAAAAATAAGTCTAAAAATTTGACGGCAGTCCGAAACGATTAAGATGCACGAAGATGTTGAAGCCCTcg                                                                     <  8:176252/148‑1 (MQ=255)
                                                                                gcagcaAGCCCTCTGTGGGCGGATGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGa                                                                  >  7:55747/1‑149 (MQ=255)
                                                                                  agcaAGGCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATTCACGAAGAGGTTGAAGCCCTCGCGATc                                                                <  8:257683/149‑1 (MQ=255)
                                                                                   gcaAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTcg                                                                     >  5:299473/1‑143 (MQ=255)
                                                                                   gcaAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTcg                                                                     <  6:299473/143‑1 (MQ=255)
                                                                                    caAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCt                                                              >  5:18591/1‑149 (MQ=255)
                                                                                      aGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTa                                                            <  4:268493/149‑1 (MQ=255)
                                                                                           tctGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGATATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCAc                                                       >  2:121137/1‑149 (MQ=255)
                                                                                               tgGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGAc                                                    <  2:202813/148‑1 (MQ=255)
                                                                                                  gcggcTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAaga                                                                                   >  8:849/1‑114 (MQ=255)
                                                                                                       tGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCaaa                                           >  1:234090/1‑149 (MQ=255)
                                                                                                       tGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCaaa                                           >  5:346290/1‑149 (MQ=255)
                                                                                                         cacaTTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAAt                                          >  1:318572/1‑148 (MQ=255)
                                                                                                          acaTTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGaa                                                                                               <  7:296457/94‑1 (MQ=255)
                                                                                                          acaTTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGaa                                                                                               >  8:296457/1‑94 (MQ=255)
                                                                                                          acaTTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAAtt                                         >  3:353856/1‑148 (MQ=255)
                                                                                                            aTTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCaa                                      <  6:18591/149‑1 (MQ=255)
                                                                                                            aTTTCCATCGCTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTc                                        >  6:198418/1‑147 (MQ=255)
                                                                                                             tttCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAg                                     <  4:353856/149‑1 (MQ=255)
                                                                                                                 cATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAAc                                  <  2:12413/148‑1 (MQ=255)
                                                                                                                     gTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGAc                             <  2:42043/149‑1 (MQ=255)
                                                                                                                          gCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTgg                        <  2:234090/149‑1 (MQ=255)
                                                                                                                              gACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGTTg                     >  6:275031/1‑148 (MQ=255)
                                                                                                                              gACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGt                      <  1:50340/147‑1 (MQ=255)
                                                                                                                                   gacttaTTTTTCATGAAAATTTACATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTgg               >  3:49148/6‑149 (MQ=255)
                                                                                                                                   gTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTgg               >  7:100798/1‑149 (MQ=255)
                                                                                                                                   gTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTgg               >  2:295774/1‑149 (MQ=255)
                                                                                                                                    tGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTGGc              <  3:140268/149‑1 (MQ=255)
                                                                                                                                    acttaTTTTTCATGAAAATTTACATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAAc                  >  5:208186/5‑145 (MQ=255)
                                                                                                                                    acttaTTTTTCATGAAAATTTACATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAAc                  <  6:208186/141‑1 (MQ=255)
                                                                                                                                      aaaTTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTGGcc             >  8:260911/1‑148 (MQ=255)
                                                                                                                                         tttttCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTGGCCGAgc         <  8:100798/149‑1 (MQ=255)
                                                                                                                                         tttttCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTGGCCGAgc         <  7:283461/149‑1 (MQ=255)
                                                                                                                                         tttttCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAAAGATGAAGATGCACGAAGAGGTTGAAGCGCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTGGCCGAgc         <  5:177195/149‑1 (MQ=255)
                                                                                                                                               aTGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTGGCCGAGCGCATc    >  2:248517/1‑148 (MQ=255)
                                                                                                                                                tGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTGGCCGAGCGCATCGa  >  2:6471/1‑149 (MQ=255)
                                                                                                                                                  tAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTGGCCGAGCGCATc    <  6:267769/145‑1 (MQ=255)
                                                                                                                                                  tAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTGGCCGAGCGCATc    >  5:267769/1‑145 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCTGGAGGATTCAGCCACGAGGCTACTCGCCGAGCGCCTGTAAAGAAACCCGCGCCACCGCAGCCAATCCGCCACGTTGCGCAGCAAGCCCTCTGTGGGCGGCTGCACATTTCCATCGTTCGGCTTGACGCGTGAAATTTTTCATGTAAATTTTCATGAAAAATAAGTCTAAAAATTTCACGGCAGTCCGAAACGATGAAGATGCACGAAGAGGTTGAAGCCCTCGCGATCCTTATTCACGACCTGCGCAAATTCAAGAACCTGACCTTGGGTGAACTGGCCGAGCGCATCGA  >  NC_002947/2482358‑2482650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑