Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,486,181 C→G 8.0% A258G (GCC→GGC)  spuC‑I → polyamine:pyruvate transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,486,1810CG 8.0% 207.8 / 4.3 69A258G (GCC→GGC) spuC‑Ipolyamine:pyruvate transaminase
Reads supporting (aligned to +/- strand):  ref base C (31/32);  new base G (2/4);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGAAGATCCTCGAAGTCGGCGTGGACAACGTTGCCGCCTTCATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGTTACATCCCCATGGGCGGGGTGATCGTGCGTGACGAGGT  >  NC_002947/2486036‑2486325
                                                                                                                                                 |                                                                                                                                                
aagaagaTCCTCGAAGTCGGCGTGGACAACGTTGCCGCCTTCATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGTCCAGGAACGACATCCTCTTAGTCGCcg                                                                                                                                                >  8:337453/1‑148 (MQ=255)
  gaagaTCCTCGAAGTCGGCGTGGACAACGTTGCCGCCTTCATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCcgac                                                                                                                                              >  5:253867/1‑148 (MQ=255)
  gaagaTCCTCGAAGTCGGCGTGGACAACGTTGCCGCCTTCATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCcgac                                                                                                                                              >  8:176663/1‑148 (MQ=255)
    agaTCCTCGAAGTCGGCGTGGACAACGTTGCCGCCTTCATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGaa                                                                                                                                           <  6:97215/149‑1 (MQ=255)
     gaTCCTCGAAGTCGGCGTGGACAACGTTGCCGCCTTCATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAg                                                                                                                                          <  1:308167/149‑1 (MQ=255)
      acccTCGACGTCGGCGTGGACAACGTTGCCGCCTTCAGCGCCGAGCCGATCCAGGGCGCCGGGGGCGTGATCATTCCGCCGCAGACCAATTGGCCGAAGATCAAGGAAATCCTGCCCAGGTACGACATCCACTTCGTCGGCGACGAAga                                                                                                                                         <  6:108087/147‑2 (MQ=18)
          tCGAAGTCGGCGTGGACAACGTTGCCGCCTTCATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGcc                                                                                                                                                 <  6:226105/137‑1 (MQ=255)
          tCGAAGTCGGCGTGGACAACGTTGCCGCCTTCATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGcc                                                                                                                                                 >  5:226105/1‑137 (MQ=255)
          tCGAAGTCGGCGTGGACAACGTTGCCGCCTTCATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATc                                                                                                                                     >  5:241918/1‑149 (MQ=255)
             aaGTCGGCGTGGACAACGTTGCCGCCTTCATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGc                                                                                                                                  <  3:319077/149‑1 (MQ=255)
                    cGTGGACAACGTTGCCGCCTTCATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCg                                                                                                                           <  3:1610/149‑1 (MQ=255)
                     gTGGACAACGTTGCCGCCTTCATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCgg                                                                                                                          <  5:247731/149‑1 (MQ=255)
                        gACAACGTTGCCGCCTTCATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCg                                                                                                                       >  7:122775/1‑149 (MQ=255)
                         aCAACGTTGCCGCCTTCATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCAGTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGACGCCGAAGAAGTGATCTCCGGGTTCGGCCg                                                                                                                       >  8:220617/1‑148 (MQ=255)
                          taaCGTTGCCGCCTTCATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTa                                                                                                                     >  7:248365/2‑149 (MQ=255)
                               gtGCCGCCTTCATCGCCGAGCCGATCCAGGGCGCCGGTGGCGGGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGt                                                                                                                <  6:295704/148‑1 (MQ=255)
                                   cgccTTCATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGt                                                                                                            >  1:229011/1‑149 (MQ=255)
                                       ttCATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCCAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGt                                                                                                                >  6:268081/1‑141 (MQ=255)
                                       ttCATCGCCGAGCCGATCCAGGGCGCCGGTGGAGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGt                                                                                                                <  5:268081/141‑1 (MQ=255)
                                         cATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGtt                                                                                                        <  2:229011/147‑1 (MQ=255)
                                         cATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTc                                                                                                       <  8:122775/148‑1 (MQ=255)
                                          aTCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGt                                                                                                                <  3:281502/138‑1 (MQ=255)
                                          aTCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGt                                                                                                                >  4:281502/1‑138 (MQ=255)
                                          aTCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCg                                                                                                      <  8:248365/148‑1 (MQ=255)
                                                gagccgaTCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGt                                                                                                                <  8:201002/132‑1 (MQ=255)
                                                gagccgaTCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGt                                                                                                                >  7:201002/1‑132 (MQ=255)
                                                     gaTCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGattatt                                                                                          <  2:157412/149‑1 (MQ=255)
                                                      aTCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGAttatta                                                                                         >  7:97483/1‑149 (MQ=255)
                                                          aGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTAcc                                                                                                                   <  5:241698/119‑1 (MQ=255)
                                                          aGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTAcc                                                                                                                   >  6:241698/1‑119 (MQ=255)
                                                          aGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGAc                                                                                     >  8:26301/1‑149 (MQ=255)
                                                                 cGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGGGAGTGGTTCGGTACCGATTATTACGACCTCAAGc                                                                              <  6:253867/149‑1 (MQ=255)
                                                                      gCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGcctgac                                                                         <  7:176663/149‑1 (MQ=255)
                                                                            tCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGAGGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCtgat                                                                    <  7:131030/148‑1 (MQ=255)
                                                                            tCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTg                                                                                                           <  6:166242/109‑1 (MQ=255)
                                                                            tCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTg                                                                                                           >  5:166242/1‑109 (MQ=255)
                                                                                   gccgcAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCg                                                            >  6:274770/1‑149 (MQ=255)
                                                                                        aGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAg                                                       >  5:206714/1‑149 (MQ=255)
                                                                                          aCCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAggg                                                     <  6:206714/149‑1 (MQ=255)
                                                                                          aCCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAggg                                                     >  6:353201/1‑149 (MQ=255)
                                                                                           ccTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGc                                                    <  5:274770/149‑1 (MQ=255)
                                                                                            cTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACg                                                                                       <  4:176516/113‑1 (MQ=255)
                                                                                               ttGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGa                                                <  8:97483/149‑1 (MQ=255)
                                                                                                  gCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCt                                             >  5:208992/1‑149 (MQ=255)
                                                                                                   ccGAAGCTCAAGGAAAGCCTGCCCAGGGACGACAGCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTGCGGCCGTACCGGTGAGTGCTTCGGTGCCGCTTATATCGACCTCAAGCGTGAGCTGAGGACCATCGCCATGGGCCTGAccgc                                            >  8:273172/1‑147 (MQ=255)
                                                                                                   ccGAAGATCAAGGACATCCTGTCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTc                                            <  7:337453/149‑1 (MQ=255)
                                                                                                     gAAGATCAAGGAAAGCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTTATCTGCGGGTTCGTGCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCg                                          <  7:220617/149‑1 (MQ=255)
                                                                                                       aGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGt                                        <  6:241918/149‑1 (MQ=255)
                                                                                                       aGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGt                                        <  6:208992/149‑1 (MQ=255)
                                                                                                        gATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGtt                                       <  7:26301/149‑1 (MQ=255)
                                                                                                           cAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCGGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGTTACa                                    >  1:94291/1‑149 (MQ=255)
                                                                                                             aGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTAGGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCgg                                         <  3:7255/142‑1 (MQ=255)
                                                                                                             aGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGAcc                                                                                    >  7:216906/1‑99 (MQ=255)
                                                                                                             aGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGAcc                                                                                    <  8:216906/99‑1 (MQ=255)
                                                                                                             aGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCgg                                         >  4:7255/1‑142 (MQ=255)
                                                                                                             aGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCGCAGGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGTTACATc                                  >  8:332385/1‑149 (MQ=255)
                                                                                                              ggAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGTTACATcc                                 >  4:163729/1‑149 (MQ=255)
                                                                                                                        gCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGTTACATCCCCATGGGc                         <  2:94291/147‑1 (MQ=255)
                                                                                                                             gTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGTTACATcccc                               >  5:234118/1‑136 (MQ=255)
                                                                                                                             gTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGTTACATcccc                               <  6:234118/136‑1 (MQ=255)
                                                                                                                             gTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGTTACATCCCCATGTGCGGGGTg                   >  8:95963/1‑148 (MQ=255)
                                                                                                                              tACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGTTACATCCCCATGGGCGGGGTGAt                 >  5:10162/1‑149 (MQ=255)
                                                                                                                              tACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATAGCCAAGGGCCTGACATCCGGTTACATCCACATGGGCGCGGTGa                  >  5:15284/1‑148 (MQ=255)
                                                                                                                                       ctctTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGTTACATCCCCATGGGCgggg                     >  5:260944/1‑136 (MQ=255)
                                                                                                                                       ctctTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGTTACATCCCCATGGGCgggg                     <  6:260944/136‑1 (MQ=255)
                                                                                                                                       cactTCGTCGGCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGTTAc                                     >  5:53259/3‑120 (MQ=255)
                                                                                                                                       cactTCGTCGGCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGTTAc                                     <  6:53259/118‑1 (MQ=255)
                                                                                                                                          ttCGTCGGCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGTTACATCCCCATGGGCGGGGTGATCGTGCGTGAc       >  7:95051/1‑147 (MQ=255)
                                                                                                                                          ttCGTCGCCGACGAGGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGTTACATCCCCATGGGCGGGGTGATCGTGCGTGAc       >  7:305428/1‑147 (MQ=255)
                                                                                                                                             gtcgGCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGTTACATCCCCATGGGCGGGGTGATCGTGCGTGACGAGGt  <  3:201025/149‑1 (MQ=255)
                                                                                                                                             gtcgGCGACGAAGAGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGTTACATCCCCATGGGCGGGGTGATCGTGCGTGACGAGGt  <  8:117522/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AAGAAGATCCTCGAAGTCGGCGTGGACAACGTTGCCGCCTTCATCGCCGAGCCGATCCAGGGCGCCGGTGGCGTGATCATTCCGCCGCAGACCTATTGGCCGAAGATCAAGGAAATCCTGGCCAGGTACGACATCCTCTTCGTCGCCGACGAAGTGATCTGCGGGTTCGGCCGTACCGGTGAGTGGTTCGGTACCGATTATTACGACCTCAAGCCTGACCTGATGACCATCGCCAAGGGCCTGACCTCCGGTTACATCCCCATGGGCGGGGTGATCGTGCGTGACGAGGT  >  NC_002947/2486036‑2486325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑