Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,488,212 G→C 7.6% intergenic (+68/‑334) PP_2182 → / → PP_2183 hypothetical protein/formate dehydrogenase subunit gamma

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,488,2120GC 7.6% 301.8 / 7.5 95intergenic (+68/‑334)PP_2182/PP_2183hypothetical protein/formate dehydrogenase subunit gamma
Reads supporting (aligned to +/- strand):  ref base G (41/46);  new base C (5/3);  total (46/49)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGTTTCAGAATGCGCCTGGTCGCTCGCATACAAC  >  NC_002947/2488064‑2488359
                                                                                                                                                     |                                                                                                                                                     
cGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAgg                                                                                                                                                       <  5:347896/149‑1 (MQ=255)
  cAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGcc                                                                                                                                                     <  6:237270/149‑1 (MQ=255)
    aTCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCg                                                                                                                                                   <  2:289034/149‑1 (MQ=255)
     tCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGCCTGCCAGACGGCAGCCCCgg                                                                                                                                                  <  1:347857/149‑1 (MQ=255)
        tGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCaa                                                                                                                                               >  1:244270/1‑149 (MQ=255)
          cGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATg                                                                                                                                             <  4:65993/149‑1 (MQ=255)
                 aaaGGCCCCGGCCCAGGCTCCGGTCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAgg                                                                                                                                     <  5:90010/149‑1 (MQ=255)
                 aaaGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAgg                                                                                                                                     >  7:192380/1‑149 (MQ=255)
                 aaaGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAgg                                                                                                                                     >  3:263899/1‑149 (MQ=255)
                 aaaGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAgg                                                                                                                                     >  2:268296/1‑149 (MQ=255)
                 aaaGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAg                                                                                                                                      <  4:53893/148‑1 (MQ=255)
                   aggcccaggcccAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGtt                                                                                                                                   <  2:244270/149‑1 (MQ=255)
                      cccaggcccaggCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCgg                                                                                                                                <  3:7057/149‑1 (MQ=255)
                       ccaggcccaggcTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGt                                                                                                                               >  1:347732/1‑149 (MQ=255)
                       ccaggcccaggcTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGt                                                                                                                               <  1:268296/149‑1 (MQ=255)
                            cccaggcTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCga                                                                                                                          <  3:271035/149‑1 (MQ=255)
                             ccaggcTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTc                                                                                                                            >  2:16592/1‑146 (MQ=255)
                              catgcTCCGGCCCCCGCCGCCGACACCGACACGGTGAAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTATCCATGCTGTATGGCATCCCATTGCCGGGGCTTCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCgaga                                                                                                                        <  4:193266/146‑1 (MQ=255)
                               aggcTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGagag                                                                                                                       >  4:21381/1‑149 (MQ=255)
                                ggcTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAgcgc                                                                                                                      >  8:170849/1‑149 (MQ=255)
                                   tCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTg                                                                                                                   >  5:139645/1‑149 (MQ=255)
                                   tCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTg                                                                                                                   >  6:352545/1‑149 (MQ=255)
                                         ccccGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATCGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGtgcat                                                                                                             >  8:216905/1‑149 (MQ=255)
                                            cgccgccgACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCCTTTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGtgcatg                                                                                                            >  1:98761/1‑148 (MQ=255)
                                            cgccgccgACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGATCTGCCCCTGAAGCCATGCTCGATGGCACCCCATTGCCGGGGCTGCCGTCTGGCAGCCCCCGCAATGGA‑CAAGGTTCGGTTTCCAGAGCCTGGtgcatg                                                                                                            >  1:103155/1‑148 (MQ=255)
                                                gccgACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGa                                                                                                      <  5:352545/149‑1 (MQ=255)
                                                 ccgACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGAt                                                                                                     >  7:117937/1‑149 (MQ=255)
                                                 ccgACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGAt                                                                                                     <  6:306478/149‑1 (MQ=255)
                                                 ccgACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATCGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGAt                                                                                                     <  7:216905/149‑1 (MQ=255)
                                                   gACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATgg                                                                                                   >  2:325471/1‑149 (MQ=255)
                                                       ccGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGttt                                                                                                >  3:136827/1‑148 (MQ=255)
                                                       ccGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTc                                                                                               <  5:198677/149‑1 (MQ=255)
                                                          cAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAg                                                                                            <  4:263899/149‑1 (MQ=255)
                                                           aaCGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGTCTGCCGTCTGGCAGCCCCGGCAATGGA‑CTAGCATCGGTTTAGACAGCCTGGTGCATCCAACGATGGTTTCAAGa                                                                                           >  2:111716/1‑149 (MQ=255)
                                                           aaCGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGa                                                                                           <  2:45575/149‑1 (MQ=255)
                                                                tGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCaa                                                                                             >  2:255833/1‑142 (MQ=255)
                                                                tGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCaa                                                                                             <  1:255833/142‑1 (MQ=255)
                                                                tGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCAc                                                                                      <  3:21381/149‑1 (MQ=255)
                                                                tGCAGGCTCCCGACTGAGCTCAGTCGCGGTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCAc                                                                                      <  2:318621/149‑1 (MQ=255)
                                                                  cattCTACCGACTGAGCTCATTCGCGTTCCGCCCC‑GTAGCCATGCTGGATGGCATCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATTGACCTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCAc                                                                                      <  1:270360/145‑1 (MQ=255)
                                                                  cAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGCGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCt                                                                                    >  2:115641/1‑149 (MQ=255)
                                                                       tACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCCACGATGGTTTCAAGACCCACCTG‑ATCg                                                                              >  5:249559/1‑149 (MQ=255)
                                                                        aCCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCaa                                                                                             >  3:52758/1‑134 (MQ=255)
                                                                        aCCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCaa                                                                                             <  4:52758/134‑1 (MQ=255)
                                                                         ccGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCAc                                                                                      <  3:65421/140‑1 (MQ=255)
                                                                         ccGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCAc                                                                                      >  4:65421/1‑140 (MQ=255)
                                                                         ccGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCg                                                                              <  7:88416/147‑1 (MQ=255)
                                                                         ccGACTGAGCTCAGGCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCg                                                                              <  5:366017/147‑1 (MQ=255)
                                                                           gACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGc                                                                            >  6:28337/1‑147 (MQ=255)
                                                                           gACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCaa                                                                          <  8:192380/149‑1 (MQ=255)
                                                                             cTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGc                                                                        <  1:325471/149‑1 (MQ=255)
                                                                                 gCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTg                                                                    <  2:347732/149‑1 (MQ=255)
                                                                                  cTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTgg                                                                   <  1:16592/149‑1 (MQ=255)
                                                                                  cTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTgg                                                                   >  2:343213/1‑149 (MQ=255)
                                                                                  cTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑AACGGCAAGCCGTgg                                                                   >  5:308295/1‑149 (MQ=255)
                                                                                  cTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCAGGCAGGCACGGCAATCGA‑CAAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATAGTATCAAGACACACCTG‑ATCGGCAAGCCGtcg                                                                   >  6:5821/1‑147 (MQ=255)
                                                                                     aGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTgg                                                                   >  8:171400/1‑146 (MQ=255)
                                                                                     aGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAg                                                                <  5:28337/149‑1 (MQ=255)
                                                                                       tCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAgat                                                              <  4:136827/149‑1 (MQ=255)
                                                                                       tCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGCGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACACACCTG‑AACGGCAAGCCGAGGCAgat                                                              >  7:284937/1‑149 (MQ=255)
                                                                                                 cccc‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCgggg                                                    >  1:38602/1‑149 (MQ=255)
                                                                                                 cccc‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCggg                                                     <  2:260009/148‑1 (MQ=255)
                                                                                                 cccc‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCAACCTG‑ATCGGCAAGCCGTGGCAGATGATGCAgggg                                                    >  6:222509/1‑149 (MQ=255)
                                                                                                  ccc‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGc                                                   <  1:343213/149‑1 (MQ=255)
                                                                                                   cc‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCg                                                  >  7:191620/1‑149 (MQ=255)
                                                                                                          ccATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGAt                                                                                                     >  6:61944/1‑93 (MQ=255)
                                                                                                           cATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTgg                                                                                                                  >  3:249799/1‑79 (MQ=255)
                                                                                                           cATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTgg                                                                                                                  <  4:249799/79‑1 (MQ=255)
                                                                                                            aTGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACg                                          >  6:159855/1‑149 (MQ=255)
                                                                                                                tGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCg                                      >  6:301438/1‑149 (MQ=255)
                                                                                                                  gATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGt                                     <  2:36841/148‑1 (MQ=255)
                                                                                                                  gATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTc                                    <  6:308295/149‑1 (MQ=255)
                                                                                                                     ggCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGGGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCggg                                 >  2:229975/1‑148 (MQ=255)
                                                                                                                        agtccATTGCCGGGCCTGCCAGACGGCAGCCCCGGCAATGGG‑CTAGGGTCGGTTTCGAGAGCCAGGTCCATGCAACGATGGTTTCAAGACCCACATG‑ATCGGCAAGGCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGgtt                               >  4:277720/4‑148 (MQ=25)
                                                                                                                        aGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGc                                                   <  3:9122/128‑1 (MQ=255)
                                                                                                                        aGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGc                                                   >  4:9122/1‑128 (MQ=255)
                                                                                                                        aGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGt                                     >  2:73746/1‑142 (MQ=255)
                                                                                                                        aGCCCATTGCCGGGGCGGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTGTTTCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGt                                     <  1:73746/143‑1 (MQ=255)
                                                                                                                         gtccATTGCCGGGCCTGCCAGACGGCAGCCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGc                                                   >  5:323577/3‑127 (MQ=37)
                                                                                                                         gtccATTGCCGGGCCTGCCAGACGGCAGCCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGc                                                   <  6:323577/125‑1 (MQ=37)
                                                                                                                          tccATTGCCGGGCCTGCCAGACGGCAGCCCCGGCAATGGG‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGttt                               >  4:334049/2‑146 (MQ=38)
                                                                                                                            cATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGTTTCAGaa                          <  2:38602/149‑1 (MQ=255)
                                                                                                                                 ccGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGTTTCAGAATgcgc                     <  5:159855/149‑1 (MQ=255)
                                                                                                                                  cGGGGCTGCCGTCTGGCAGGCTCGGCAATGTA‑CTTGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGTTTCATAATGCGcc                    <  5:5821/149‑1 (MQ=255)
                                                                                                                                  cGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTgg                                                                   >  3:352622/1‑102 (MQ=255)
                                                                                                                                  cGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTgg                                                                   <  4:352622/102‑1 (MQ=255)
                                                                                                                                   ggggCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGc                                                                        <  6:168677/96‑1 (MQ=255)
                                                                                                                                   ggggCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGc                                                                        >  5:168677/1‑96 (MQ=255)
                                                                                                                                   ggggCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGTTTCAGAATGCGCCt                   <  2:98761/149‑1 (MQ=255)
                                                                                                                                   ggggCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGTTTCAGAATGCGCCt                   <  2:103155/149‑1 (MQ=255)
                                                                                                                                   gcgccggCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGATCGGGTTCCAGAGCCTGGTGCATGCAACGACGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATCCCGGGGCGTAATCACGTGCGTCGGTTTCAGAATGCGCCt                   <  8:331581/143‑1 (MQ=255)
                                                                                                                                               cggCAGCCCCGGCAATGGG‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGTTTCAGAATGCGCCTGGTCGCTCGCAt       <  3:334049/148‑1 (MQ=255)
                                                                                                                                                 gCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGTTTCAGAATGCGCCTGGTCGCTCGCATa      >  6:237642/1‑148 (MQ=255)
                                                                                                                                                 gCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGTTTCAGAATGCGCCTGGTCGCTCGCATa      >  2:122129/1‑148 (MQ=255)
                                                                                                                                                   aGGCCCGGCTATGGA‑CTAGGTTCGCGTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGGTTCAGAATGCGCCTGGGCGCTCGCATACaa   <  6:249559/149‑1 (MQ=255)
                                                                                                                                                   aGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGTTTCAGAATGCGCCTGGTCGCTCGCATACaa   >  7:142067/1‑149 (MQ=255)
                                                                                                                                                     gCCCGGCAATGGA‑CTAGGTTCGGTTTCGCGAGCCTGGTGCATGCATGGTTGGTTTCATGACCGACCTG‑ATCGGCTAGCCGTGGCAGATGATGCCGGGGCGTTTTCAGGTGCGTCGGTTTCAGAATGCGCCTGGGCGGTCGCATACAAc  <  8:284937/148‑1 (MQ=255)
                                                                                                                                                     gCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGTTTCAGAATGCGcc                    >  5:217353/1‑130 (MQ=255)
                                                                                                                                                     gCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGTTTCAGAATGCGcc                    <  6:217353/130‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCAATCGTGCGAAGTCAAAGGCCCAGGCCCAGGCTCCGGCCCCCGCCGCCGACACCGCAACGGTGCAGTCTACCGACTGAGCTCAGTCGCGTTCTGCCCC‑GTAGCCATGCTGGATGGCAGCCCATTGCCGGGGCTGCCGTCTGGCAGGCCCGGCAATGGA‑CTAGGTTCGGTTTCGAGAGCCTGGTGCATGCAACGATGGTTTCAAGACCCACCTG‑ATCGGCAAGCCGTGGCAGATGATGCCGGGGCGTAATCACGTGCGTCGGTTTCAGAATGCGCCTGGTCGCTCGCATACAAC  >  NC_002947/2488064‑2488359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑