Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,524,526	C→G	9.8%	T416T (ACC→ACG)	PP_2213 →	acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,524,526	0	C	G	9.8%	239.8 / 11.7	80	T416T (ACC→ACG)	PP_2213	acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (37/35);  new base G (3/5);  total (40/40)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TTCTGCCGGCTATGCCATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATC  >  NC_002947/2524379‑2524673                                                                                                                                                     |                                                                                                                                                    ttCTGCCGGCTATGCCATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGAcc                                                                                                                                                     >  2:303145/1‑148 (MQ=255)   cTGCCGGCTATGCCATCCCCGGCCACCGCAGCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCaa                                                                                                                                                   <  3:23607/148‑1 (MQ=255)    tGCCGGCTATGCCATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTGGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACGaagg                                                                                                                                                 >  2:206564/1‑147 (MQ=255)       cGGCTATGCCATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCt                                                                                                                                              >  5:147303/1‑149 (MQ=255)             tGCCATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTtcgt                                                                                                                                          <  6:147303/147‑1 (MQ=255)               ccATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCgg                                                                                                                                       >  5:114250/1‑148 (MQ=255)               ccATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGc                                                                                                                                      <  3:3123/149‑1 (MQ=255)               ccATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGc                                                                                                                                      <  1:303145/149‑1 (MQ=255)                 aTCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACGAAGGCTTTGGTCGGCag                                                                                                                                    <  1:267652/149‑2 (MQ=255)                 aTCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGc                                                                                                                                      >  6:155451/1‑147 (MQ=255)                 aTCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTGGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACGAAGGCTTTGGTCGGCag                                                                                                                                    <  1:206564/149‑2 (MQ=255)                 aTCCCCGGCCACCGCATCGTGGTGGGGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACGAAGGCTTTGGTCGGCag                                                                                                                                    <  1:272142/149‑2 (MQ=255)                     ccGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGtac                                                                                                                                >  1:145744/1‑149 (MQ=255)                      cGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCAAGCCCGGCAACCTCGCCGTTCACCGTAAGCAGTCCACGCTGAGATGGTTCGGCGCTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAACtac                                                                                                                                >  4:193204/1‑148 (MQ=255)                       ggCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGCTCAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGtac                                                                                                                                >  1:68999/1‑148 (MQ=255)                              gCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAgc                                                                                                                       <  7:242280/149‑1 (MQ=255)                              gCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCAGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAgc                                                                                                                       >  1:155198/1‑149 (MQ=255)                                 tCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑TAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAgcggc                                                                                                                    <  3:24294/149‑1 (MQ=255)                                   gtggtggtgGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGa                                                                                                                  <  6:114250/149‑1 (MQ=255)                                      gtggtggACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGacac                                                                                                               <  6:287673/149‑1 (MQ=255)                                       gggtggACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACt                                                                                                              <  8:323268/148‑1 (MQ=255)                                         gtggACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCgg                                                                                                                                       <  4:255982/122‑1 (MQ=255)                                         gtggACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCgg                                                                                                                                       >  3:255982/1‑122 (MQ=255)                                              cGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGc                                                                                                       <  2:145744/149‑1 (MQ=255)                                               gAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCt                                                                                                      >  7:197228/1‑149 (MQ=255)                                               gAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCt                                                                                                      >  5:342353/1‑149 (MQ=255)                                                 gCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTg                                                                                                     >  7:12612/1‑148 (MQ=255)                                                 gCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGa                                                                                                    <  5:155451/149‑1 (MQ=255)                                                 gCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGACATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGC‑TCGACAGTGTCgccgctga                                                                                                   <  5:161111/149‑8 (MQ=255)                                                            aaCTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGgc                                                                                         <  3:31507/149‑1 (MQ=255)                                                                gC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGgcagca                                                                                     >  5:254970/1‑149 (MQ=255)                                                                   cAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATc                                                                                   <  4:175983/149‑1 (MQ=255)                                                                    aGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCa                                                                                  <  2:68999/149‑1 (MQ=255)                                                                       cGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGCTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCCGATGGCAGCATCAGCt                                                                               >  6:20321/1‑149 (MQ=255)                                                                           cagccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGt                                                                           >  2:147323/1‑149 (MQ=255)                                                                            agccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGa                                                                                                    >  8:45245/1‑123 (MQ=255)                                                                            agccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGa                                                                                                    <  7:45245/123‑1 (MQ=255)                                                                              ccagGCATCCTCGCCGTTGACCTTGAGCAGTCGCAGCACTCCTGCTTCCGCGGGTACCACGTCCTACCGACCAAAGCCATCGTCGGCAAGTACTACCGCAGCCGCTCCCATGACGCGCTGAAACCGAATGGAAGCATCAGCTTCGTTgg                                                                        >  6:274160/1‑149 (MQ=255)                                                                              ccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCaaa                                                                                                                                                  >  8:93685/1‑75 (MQ=255)                                                                              ccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCaaa                                                                                                                                                  <  7:93685/75‑1 (MQ=255)                                                                              ccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATg                                                                                           >  4:50977/1‑130 (MQ=255)                                                                              ccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATg                                                                                           <  3:50977/130‑1 (MQ=255)                                                                              ccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTgg                                                                        >  6:280380/1‑149 (MQ=255)                                                                                agGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCCAAGCCTTCGTCGGCAAGTACTACCTCAgcggc                                                                                                                    <  2:45782/103‑1 (MQ=255)                                                                                agGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCCAAGCCTTCGTCGGCAAGTACTACCTCAgcggc                                                                                                                    >  1:45782/1‑103 (MQ=255)                                                                                agGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGcc                                                                      <  2:26366/149‑1 (MQ=255)                                                                                  gCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTAGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCg                                                                     >  6:70116/1‑148 (MQ=255)                                                                                     tCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACTGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGc                                                                 <  3:193204/149‑1 (MQ=255)                                                                                     tCCTCGCCGTTGACCGTGAGCAGTCGCCGCTCTGCTGGTTCCGCGGTTACCACGGCCTGCCGACCAAAGCCATCGTCGGCAAgaa                                                                                                                                 >  5:125462/1‑83 (MQ=255)                                                                                     acgtCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACGAAAGCCTTCGTCGGCAAGta                                                                                                                                 <  6:125462/82‑1 (MQ=255)                                                                                        tCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACGACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGcgac                                                              >  5:108849/1‑149 (MQ=255)                                                                                        tCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGcgac                                                              >  6:307613/1‑149 (MQ=255)                                                                                        tCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGcgac                                                              >  2:33141/1‑149 (MQ=255)                                                                                             gTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTTAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGt                                                         <  2:155198/149‑1 (MQ=255)                                                                                                 aCCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGcc                                                                      <  1:72706/132‑1 (MQ=255)                                                                                                 aCCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGcc                                                                      >  2:72706/1‑132 (MQ=255)                                                                                                 aCCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGAt                                                      <  5:70116/148‑1 (MQ=255)                                                                                                 aCCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGAt                                                      >  3:167270/1‑148 (MQ=255)                                                                                                          aGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGAt                                                      <  1:306323/139‑1 (MQ=255)                                                                                                          aGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGAt                                                      >  2:306323/1‑139 (MQ=255)                                                                                                            tCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCgg                                          >  7:62444/1‑149 (MQ=255)                                                                                                              gccgcTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGt                                         >  6:45144/1‑148 (MQ=255)                                                                                                                     tgCTTGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCgtgt                                 <  3:262031/149‑1 (MQ=255)                                                                                                                     tgCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCgt                                   <  8:62444/147‑1 (MQ=255)                                                                                                                          gTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGc                            >  2:276258/1‑149 (MQ=255)                                                                                                                               gcggTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTc                       >  8:94621/1‑149 (MQ=255)                                                                                                                               gcggTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTc                       <  5:280380/149‑1 (MQ=255)                                                                                                                               gcggTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTc                       >  3:78481/1‑149 (MQ=255)                                                                                                                                  gTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGAt                    >  5:36503/1‑149 (MQ=255)                                                                                                                                  gTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGAt                    <  1:276258/149‑1 (MQ=255)                                                                                                                                   ttACAACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGAtg                   >  5:10091/1‑149 (MQ=255)                                                                                                                                      ccACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTgg                <  6:36503/149‑1 (MQ=255)                                                                                                                                       cACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTgg                >  5:190546/1‑148 (MQ=255)                                                                                                                                         cGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGaaa             <  6:254970/149‑1 (MQ=255)                                                                                                                                           gCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAgc           <  6:190546/149‑1 (MQ=255)                                                                                                                                           acttGCCGACGAAGGCTTTGGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAgc           >  5:320211/4‑149 (MQ=255)                                                                                                                                             ttGCCGACGAAGGCTTTGGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGAtgtg                 <  2:31121/140‑1 (MQ=255)                                                                                                                                             ttGCCGACGAAGGCTTTGGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGAtgtg                 >  1:31121/2‑141 (MQ=255)                                                                                                                                                ccGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGtt      >  4:236221/1‑149 (MQ=255)                                                                                                                                                ccGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGtt      <  5:20321/149‑1 (MQ=255)                                                                                                                                                ccGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGt       <  5:45144/148‑1 (MQ=255)                                                                                                                                                    ccAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTTGGGCCATTCGATGTGGAAAGCGCGTTGATc  <  1:33141/149‑1 (MQ=255)                                                                                                                                                    ccAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATc  <  7:94621/149‑1 (MQ=255)                                                                                                                                                     cAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATc  >  5:306222/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TTCTGCCGGCTATGCCATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATC  >  NC_002947/2524379‑2524673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑