Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,524,532	C→T	7.2%	A418A (GCC→GCT)	PP_2213 →	acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,524,532	0	C	T	7.2%	239.6 / 5.6	83	A418A (GCC→GCT)	PP_2213	acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (39/38);  new base T (2/4);  total (41/42)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.46e-01

CGGCTATGCCATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATCGAGCA  >  NC_002947/2524385‑2524678                                                                                                                                                     |                                                                                                                                                   cGGCTATGCCATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCt                                                                                                                                                   >  5:147303/1‑149 (MQ=255)       tGCCATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTtcgt                                                                                                                                               <  6:147303/147‑1 (MQ=255)         ccATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCgg                                                                                                                                            >  5:114250/1‑148 (MQ=255)         ccATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGc                                                                                                                                           <  1:303145/149‑1 (MQ=255)         ccATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGc                                                                                                                                           <  3:3123/149‑1 (MQ=255)           aTCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACGAAGGCTTTGGTCGGCag                                                                                                                                         <  1:267652/149‑2 (MQ=255)           aTCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGc                                                                                                                                           >  6:155451/1‑147 (MQ=255)           aTCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTGGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACGAAGGCTTTGGTCGGCag                                                                                                                                         <  1:206564/149‑2 (MQ=255)           aTCCCCGGCCACCGCATCGTGGTGGGGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACGAAGGCTTTGGTCGGCag                                                                                                                                         <  1:272142/149‑2 (MQ=255)               ccGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGtac                                                                                                                                     >  1:145744/1‑149 (MQ=255)                cGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCAAGCCCGGCAACCTCGCCGTTCACCGTAAGCAGTCCACGCTGAGATGGTTCGGCGCTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAACtac                                                                                                                                     >  4:193204/1‑148 (MQ=255)                 ggCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGCTCAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGtac                                                                                                                                     >  1:68999/1‑148 (MQ=255)                        gCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAgc                                                                                                                            <  7:242280/149‑1 (MQ=255)                        gCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCAGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAgc                                                                                                                            >  1:155198/1‑149 (MQ=255)                           tCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑TAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAgcggc                                                                                                                         <  3:24294/149‑1 (MQ=255)                             gtggtggtgGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGa                                                                                                                       <  6:114250/149‑1 (MQ=255)                                gtggtggACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGacac                                                                                                                    <  6:287673/149‑1 (MQ=255)                                 gggtggACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACt                                                                                                                   <  8:323268/148‑1 (MQ=255)                                   gtggACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCgg                                                                                                                                            <  4:255982/122‑1 (MQ=255)                                   gtggACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCgg                                                                                                                                            >  3:255982/1‑122 (MQ=255)                                        cGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGc                                                                                                            <  2:145744/149‑1 (MQ=255)                                         gAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCt                                                                                                           >  5:342353/1‑149 (MQ=255)                                         gAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCt                                                                                                           >  7:197228/1‑149 (MQ=255)                                           gCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTg                                                                                                          >  7:12612/1‑148 (MQ=255)                                           gCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGa                                                                                                         <  5:155451/149‑1 (MQ=255)                                           gCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGACATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGC‑TCGACAGTGTCgccgctga                                                                                                        <  5:161111/149‑8 (MQ=255)                                                      aaCTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGgc                                                                                              <  3:31507/149‑1 (MQ=255)                                                          gC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGgcagca                                                                                          >  5:254970/1‑149 (MQ=255)                                                             cAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATc                                                                                        <  4:175983/149‑1 (MQ=255)                                                              aGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCa                                                                                       <  2:68999/149‑1 (MQ=255)                                                                 cGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGCTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCCGATGGCAGCATCAGCt                                                                                    >  6:20321/1‑149 (MQ=255)                                                                     cagccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGt                                                                                >  2:147323/1‑149 (MQ=255)                                                                      agccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGa                                                                                                         >  8:45245/1‑123 (MQ=255)                                                                      agccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGa                                                                                                         <  7:45245/123‑1 (MQ=255)                                                                        ccagGCATCCTCGCCGTTGACCTTGAGCAGTCGCAGCACTCCTGCTTCCGCGGGTACCACGTCCTACCGACCAAAGCCATCGTCGGCAAGTACTACCGCAGCCGCTCCCATGACGCGCTGAAACCGAATGGAAGCATCAGCTTCGTTgg                                                                             >  6:274160/1‑149 (MQ=255)                                                                        ccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATg                                                                                                <  3:50977/130‑1 (MQ=255)                                                                        ccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATg                                                                                                >  4:50977/1‑130 (MQ=255)                                                                        ccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTgg                                                                             >  6:280380/1‑149 (MQ=255)                                                                          agGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCCAAGCCTTCGTCGGCAAGTACTACCTCAgcggc                                                                                                                         >  1:45782/1‑103 (MQ=255)                                                                          agGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCCAAGCCTTCGTCGGCAAGTACTACCTCAgcggc                                                                                                                         <  2:45782/103‑1 (MQ=255)                                                                          agGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGcc                                                                           <  2:26366/149‑1 (MQ=255)                                                                            gCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTAGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCg                                                                          >  6:70116/1‑148 (MQ=255)                                                                               tCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACTGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGc                                                                      <  3:193204/149‑1 (MQ=255)                                                                               tCCTCGCCGTTGACCGTGAGCAGTCGCCGCTCTGCTGGTTCCGCGGTTACCACGGCCTGCCGACCAAAGCCATCGTCGGCAAgaa                                                                                                                                      >  5:125462/1‑83 (MQ=255)                                                                               acgtCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACGAAAGCCTTCGTCGGCAAGta                                                                                                                                      <  6:125462/82‑1 (MQ=255)                                                                                  tCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACGACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGcgac                                                                   >  5:108849/1‑149 (MQ=255)                                                                                  tCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGcgac                                                                   >  6:307613/1‑149 (MQ=255)                                                                                  tCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGcgac                                                                   >  2:33141/1‑149 (MQ=255)                                                                                       gTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTTAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGt                                                              <  2:155198/149‑1 (MQ=255)                                                                                           aCCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGcc                                                                           <  1:72706/132‑1 (MQ=255)                                                                                           aCCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGcc                                                                           >  2:72706/1‑132 (MQ=255)                                                                                           aCCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGAt                                                           >  3:167270/1‑148 (MQ=255)                                                                                           aCCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGAt                                                           <  5:70116/148‑1 (MQ=255)                                                                                                    aGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGAt                                                           >  2:306323/1‑139 (MQ=255)                                                                                                    aGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGAt                                                           <  1:306323/139‑1 (MQ=255)                                                                                                      tCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCgg                                               >  7:62444/1‑149 (MQ=255)                                                                                                        gccgcTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGt                                              >  6:45144/1‑148 (MQ=255)                                                                                                               tgCTTGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCgtgt                                      <  3:262031/149‑1 (MQ=255)                                                                                                               tgCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCgt                                        <  8:62444/147‑1 (MQ=255)                                                                                                                    gTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGc                                 >  2:276258/1‑149 (MQ=255)                                                                                                                         gcggTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTc                            >  8:94621/1‑149 (MQ=255)                                                                                                                         gcggTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTc                            >  3:78481/1‑149 (MQ=255)                                                                                                                         gcggTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTc                            <  5:280380/149‑1 (MQ=255)                                                                                                                            gTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGAt                         >  5:36503/1‑149 (MQ=255)                                                                                                                            gTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGAt                         <  1:276258/149‑1 (MQ=255)                                                                                                                             ttACAACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGAtg                        >  5:10091/1‑149 (MQ=255)                                                                                                                                ccACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTgg                     <  6:36503/149‑1 (MQ=255)                                                                                                                                 cACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTgg                     >  5:190546/1‑148 (MQ=255)                                                                                                                                   cGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGaaa                  <  6:254970/149‑1 (MQ=255)                                                                                                                                     gCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAgc                <  6:190546/149‑1 (MQ=255)                                                                                                                                     acttGCCGACGAAGGCTTTGGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAgc                >  5:320211/4‑149 (MQ=255)                                                                                                                                       ttGCCGACGAAGGCTTTGGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGAtgtg                      >  1:31121/2‑141 (MQ=255)                                                                                                                                       ttGCCGACGAAGGCTTTGGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGAtgtg                      <  2:31121/140‑1 (MQ=255)                                                                                                                                          ccGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGtt           <  5:20321/149‑1 (MQ=255)                                                                                                                                          ccGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGtt           >  4:236221/1‑149 (MQ=255)                                                                                                                                          ccGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGt            <  5:45144/148‑1 (MQ=255)                                                                                                                                              ccAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTTGGGCCATTCGATGTGGAAAGCGCGTTGATc       <  1:33141/149‑1 (MQ=255)                                                                                                                                              ccAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATc       <  7:94621/149‑1 (MQ=255)                                                                                                                                               cAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATc       >  5:306222/1‑148 (MQ=255)                                                                                                                                                aaaGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATc       >  6:183952/1‑147 (MQ=255)                                                                                                                                                aaaGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATCGa     <  1:346931/149‑1 (MQ=255)                                                                                                                                                 aaGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATCGAg    >  2:109771/1‑149 (MQ=255)                                                                                                                                                   gCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATCGAGCa  <  4:78481/149‑1 (MQ=255)                                                                                                                                                     cTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTgggg                                  <  3:129195/115‑1 (MQ=255)                                                                                                                                                     cTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTgggg                                  >  4:129195/1‑115 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CGGCTATGCCATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATCGAGCA  >  NC_002947/2524385‑2524678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑