Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,524,535	C→G	7.2%	F419L (TTC→TTG)	PP_2213 →	acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,524,535	0	C	G	7.2%	263.0 / 7.8	83	F419L (TTC→TTG)	PP_2213	acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (38/38);  new base G (2/4);  total (41/42)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

TGCCATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATCGAGCACCCG  >  NC_002947/2524391‑2524682                                                                                                                                                  |                                                                                                                                                    tGCCATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTtcgt                                                                                                                                                   <  6:147303/147‑1 (MQ=255)   ccATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCgg                                                                                                                                                >  5:114250/1‑148 (MQ=255)   ccATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGc                                                                                                                                               <  1:303145/149‑1 (MQ=255)   ccATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGc                                                                                                                                               <  3:3123/149‑1 (MQ=255)     aTCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACGAAGGCTTTGGTCGGCag                                                                                                                                             <  1:267652/149‑2 (MQ=255)     aTCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGc                                                                                                                                               >  6:155451/1‑147 (MQ=255)     aTCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTGGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACGAAGGCTTTGGTCGGCag                                                                                                                                             <  1:206564/149‑2 (MQ=255)     aTCCCCGGCCACCGCATCGTGGTGGGGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACGAAGGCTTTGGTCGGCag                                                                                                                                             <  1:272142/149‑2 (MQ=255)         ccGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGtac                                                                                                                                         >  1:145744/1‑149 (MQ=255)          cGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCAAGCCCGGCAACCTCGCCGTTCACCGTAAGCAGTCCACGCTGAGATGGTTCGGCGCTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAACtac                                                                                                                                         >  4:193204/1‑148 (MQ=255)           ggCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGCTCAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGtac                                                                                                                                         >  1:68999/1‑148 (MQ=255)                  gCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAgc                                                                                                                                <  7:242280/149‑1 (MQ=255)                  gCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCAGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAgc                                                                                                                                >  1:155198/1‑149 (MQ=255)                     tCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑TAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAgcggc                                                                                                                             <  3:24294/149‑1 (MQ=255)                       gtggtggtgGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGa                                                                                                                           <  6:114250/149‑1 (MQ=255)                          gtggtggACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGacac                                                                                                                        <  6:287673/149‑1 (MQ=255)                           gggtggACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACt                                                                                                                       <  8:323268/148‑1 (MQ=255)                             gtggACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCgg                                                                                                                                                <  4:255982/122‑1 (MQ=255)                             gtggACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCgg                                                                                                                                                >  3:255982/1‑122 (MQ=255)                                  cGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGc                                                                                                                <  2:145744/149‑1 (MQ=255)                                   gAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCt                                                                                                               >  5:342353/1‑149 (MQ=255)                                   gAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCt                                                                                                               >  7:197228/1‑149 (MQ=255)                                     gCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTg                                                                                                              >  7:12612/1‑148 (MQ=255)                                     gCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGa                                                                                                             <  5:155451/149‑1 (MQ=255)                                     gCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGACATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGC‑TCGACAGTGTCgccgctga                                                                                                            <  5:161111/149‑8 (MQ=255)                                                aaCTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGgc                                                                                                  <  3:31507/149‑1 (MQ=255)                                                    gC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGgcagca                                                                                              >  5:254970/1‑149 (MQ=255)                                                       cAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATc                                                                                            <  4:175983/149‑1 (MQ=255)                                                        aGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCa                                                                                           <  2:68999/149‑1 (MQ=255)                                                           cGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGCTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCCGATGGCAGCATCAGCt                                                                                        >  6:20321/1‑149 (MQ=255)                                                               cagccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGt                                                                                    >  2:147323/1‑149 (MQ=255)                                                                agccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGa                                                                                                             <  7:45245/123‑1 (MQ=255)                                                                agccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGa                                                                                                             >  8:45245/1‑123 (MQ=255)                                                                  ccagGCATCCTCGCCGTTGACCTTGAGCAGTCGCAGCACTCCTGCTTCCGCGGGTACCACGTCCTACCGACCAAAGCCATCGTCGGCAAGTACTACCGCAGCCGCTCCCATGACGCGCTGAAACCGAATGGAAGCATCAGCTTCGTTgg                                                                                 >  6:274160/1‑149 (MQ=255)                                                                  ccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATg                                                                                                    <  3:50977/130‑1 (MQ=255)                                                                  ccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATg                                                                                                    >  4:50977/1‑130 (MQ=255)                                                                  ccagGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTgg                                                                                 >  6:280380/1‑149 (MQ=255)                                                                    agGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCCAAGCCTTCGTCGGCAAGTACTACCTCAgcggc                                                                                                                             >  1:45782/1‑103 (MQ=255)                                                                    agGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCCAAGCCTTCGTCGGCAAGTACTACCTCAgcggc                                                                                                                             <  2:45782/103‑1 (MQ=255)                                                                    agGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGcc                                                                               <  2:26366/149‑1 (MQ=255)                                                                      gCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTAGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCg                                                                              >  6:70116/1‑148 (MQ=255)                                                                         tCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACTGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGc                                                                          <  3:193204/149‑1 (MQ=255)                                                                         tCCTCGCCGTTGACCGTGAGCAGTCGCCGCTCTGCTGGTTCCGCGGTTACCACGGCCTGCCGACCAAAGCCATCGTCGGCAAgaa                                                                                                                                          >  5:125462/1‑83 (MQ=255)                                                                         acgtCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACGAAAGCCTTCGTCGGCAAGta                                                                                                                                          <  6:125462/82‑1 (MQ=255)                                                                            tCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACGACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGcgac                                                                       >  5:108849/1‑149 (MQ=255)                                                                            tCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGcgac                                                                       >  6:307613/1‑149 (MQ=255)                                                                            tCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGcgac                                                                       >  2:33141/1‑149 (MQ=255)                                                                                 gTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTTAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGt                                                                  <  2:155198/149‑1 (MQ=255)                                                                                     aCCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGcc                                                                               >  2:72706/1‑132 (MQ=255)                                                                                     aCCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGcc                                                                               <  1:72706/132‑1 (MQ=255)                                                                                     aCCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGAt                                                               <  5:70116/148‑1 (MQ=255)                                                                                     aCCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGAt                                                               >  3:167270/1‑148 (MQ=255)                                                                                              aGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGAt                                                               >  2:306323/1‑139 (MQ=255)                                                                                              aGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGAt                                                               <  1:306323/139‑1 (MQ=255)                                                                                                tCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCgg                                                   >  7:62444/1‑149 (MQ=255)                                                                                                  gccgcTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGt                                                  >  6:45144/1‑148 (MQ=255)                                                                                                         tgCTTGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCgtgt                                          <  3:262031/149‑1 (MQ=255)                                                                                                         tgCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCgt                                            <  8:62444/147‑1 (MQ=255)                                                                                                              gTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGc                                     >  2:276258/1‑149 (MQ=255)                                                                                                                   gcggTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTc                                >  8:94621/1‑149 (MQ=255)                                                                                                                   gcggTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTc                                >  3:78481/1‑149 (MQ=255)                                                                                                                   gcggTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTc                                <  5:280380/149‑1 (MQ=255)                                                                                                                      gTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGAt                             >  5:36503/1‑149 (MQ=255)                                                                                                                      gTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGAt                             <  1:276258/149‑1 (MQ=255)                                                                                                                       ttACAACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGAtg                            >  5:10091/1‑149 (MQ=255)                                                                                                                          ccACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTgg                         <  6:36503/149‑1 (MQ=255)                                                                                                                           cACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTgg                         >  5:190546/1‑148 (MQ=255)                                                                                                                             cGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGaaa                      <  6:254970/149‑1 (MQ=255)                                                                                                                               gCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAgc                    <  6:190546/149‑1 (MQ=255)                                                                                                                               acttGCCGACGAAGGCTTTGGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAgc                    >  5:320211/4‑149 (MQ=255)                                                                                                                                 ttGCCGACGAAGGCTTTGGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGAtgtg                          >  1:31121/2‑141 (MQ=255)                                                                                                                                 ttGCCGACGAAGGCTTTGGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGAtgtg                          <  2:31121/140‑1 (MQ=255)                                                                                                                                    ccGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGtt               <  5:20321/149‑1 (MQ=255)                                                                                                                                    ccGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGtt               >  4:236221/1‑149 (MQ=255)                                                                                                                                    ccGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGt                <  5:45144/148‑1 (MQ=255)                                                                                                                                        ccAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTTGGGCCATTCGATGTGGAAAGCGCGTTGATc           <  1:33141/149‑1 (MQ=255)                                                                                                                                        ccAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATc           <  7:94621/149‑1 (MQ=255)                                                                                                                                         cAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATc           >  5:306222/1‑148 (MQ=255)                                                                                                                                          aaaGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATc           >  6:183952/1‑147 (MQ=255)                                                                                                                                          aaaGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATCGa         <  1:346931/149‑1 (MQ=255)                                                                                                                                           aaGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATCGAg        >  2:109771/1‑149 (MQ=255)                                                                                                                                             gCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATCGAGCa      <  4:78481/149‑1 (MQ=255)                                                                                                                                               cTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTgggg                                      <  3:129195/115‑1 (MQ=255)                                                                                                                                               cTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTgggg                                      >  4:129195/1‑115 (MQ=255)                                                                                                                                                  cgtcgGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATCGAGCACCcg  <  8:12612/148‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    TGCCATCCCCGGCCACCGCATCGTGGTGGTGGACGAGCAAGGTAACGAACTGC‑CAGCCGGCCAGCCAGGCATCCTCGCCGTTGACCGTGAGCAGTCGCCGCTGTGCTGGTTCGGCGGTTACCACGGCCTGCCGACCAAAGCCTTCGTCGGCAAGTACTACCTCAGCGGCGACACTGTCGAGCTGAACCCGGATGGCAGCATCAGCTTCGTTGGCCGTAGCGACGACGTGATCACCACCTCCGGTTACCGTGTGGGGCCATTCGATGTGGAAAGCGCGTTGATCGAGCACCCG  >  NC_002947/2524391‑2524682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑