Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,568,704 T→A 8.0% M282L (ATG→TTG)  PP_2252 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,568,7040TA 8.0% 218.9 / 6.8 75M282L (ATG→TTG) PP_2252hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (40/29);  new base A (3/3);  total (43/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

GGCGCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTCGCTGGCCTG  >  NC_002947/2568562‑2568851
                                                                                                                                               |                                                                                                                                                   
ggcgCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGATGGTCGAACAACTGCgg                                                                                                                                               <  2:338305/149‑1 (MQ=255)
 gcgcACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCggg                                                                                                                                              <  8:270670/149‑1 (MQ=255)
    cACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGggtgg                                                                                                                                           <  6:286324/149‑1 (MQ=255)
     aCTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGggtggt                                                                                                                                          >  7:45571/1‑149 (MQ=255)
        ggCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCAc                                                                                                                                       >  8:253762/1‑149 (MQ=255)
          ccAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACgg                                                                                                                                     <  8:2433/149‑1 (MQ=255)
           cAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGt                                                                                                                                    >  2:263250/1‑149 (MQ=255)
                     tCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGtca                                                                                                                           >  3:156598/1‑148 (MQ=255)
                     tCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGtc                                                                                                                            <  1:190468/147‑1 (MQ=255)
                       cTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGtcatc                                                                                                                         <  8:45571/148‑1 (MQ=255)
                        tCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCAc                                                                                                                       >  7:105816/1‑149 (MQ=255)
                        tCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCAc                                                                                                                       >  3:112050/1‑149 (MQ=255)
                          aaCCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCAcc                                                                                                                      <  1:263250/148‑1 (MQ=255)
                            ccTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCg                                                                                                                   <  3:150365/149‑1 (MQ=255)
                             cTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCg                                                                                                                   >  5:304940/1‑148 (MQ=255)
                                 cccGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAAcc                                                                                                              >  6:291128/1‑149 (MQ=255)
                                         gTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCgg                                                                                                      <  6:304940/149‑1 (MQ=255)
                                           aaTAGACCCCCGCCATGGCCCCGCTCTGGCGGGTCTCCATCAACTCCTGCCATTCCTGGTTCACCGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGGCATCACCCCGTAACCGGCTGCGGc                                                                                                     >  4:31673/1‑148 (MQ=255)
                                            aTAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGc                                                                                                     >  6:149481/1‑147 (MQ=255)
                                            aTAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGc                                                                                                     <  5:149481/147‑1 (MQ=255)
                                              agccccccGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCCCCCCGTAACCGGCTGCGGCAATc                                                                                                 >  5:301961/4‑149 (MQ=255)
                                              aGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATc                                                                                                 >  2:166556/1‑149 (MQ=255)
                                                aCCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCg                                                                                                >  3:34306/1‑148 (MQ=255)
                                                 cccccGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGa                                                                                               >  7:76536/1‑148 (MQ=255)
                                                  ccccGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACg                                                                                                                                      >  7:199928/1‑108 (MQ=255)
                                                  ccccGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACg                                                                                                                                      >  7:194697/1‑108 (MQ=255)
                                                  ccccGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACg                                                                                                                                      <  8:199928/108‑1 (MQ=255)
                                                  ccccGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACg                                                                                                                                      <  8:194697/108‑1 (MQ=255)
                                                  ccccGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGaaa                                                                                             >  8:214345/1‑149 (MQ=255)
                                                  ccccGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGaaa                                                                                             <  7:253762/149‑1 (MQ=255)
                                                         aTGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGc                                                                                      >  2:245184/1‑149 (MQ=255)
                                                              cccGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTg                                                                                 >  4:136358/1‑149 (MQ=255)
                                                                       gCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCtt                                                                          <  7:214345/147‑1 (MQ=255)
                                                                        cGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGcc                                                                       <  8:76536/149‑1 (MQ=255)
                                                                             ctcCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTg                                                                  >  2:187864/1‑149 (MQ=255)
                                                                                cATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGcc                                                                >  1:266078/1‑149 (MQ=255)
                                                                                cATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGcc                                                                >  7:178100/1‑148 (MQ=255)
                                                                                cATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGcc                                                                >  5:301811/1‑148 (MQ=255)
                                                                                       tCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCAc                                                        >  3:149152/1‑149 (MQ=255)
                                                                                         cTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCAc                                                        <  4:156598/147‑1 (MQ=255)
                                                                                         cTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACgg                                                      >  2:82704/1‑149 (MQ=255)
                                                                                          tGCCATTCCTGGTTCAACGACACGTTCAGGATAACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACTGTCATGGGTCATCACCCCGTAAACGGCAGCGGCAATCGAACTCATCGCCCCTGCCACCTTGCACACTGCCGATGCCAAGGc                                                     >  4:104497/1‑149 (MQ=255)
                                                                                            ccATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCAc                                                   >  3:275051/1‑149 (MQ=255)
                                                                                                  cTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATg                                                            >  5:122196/1‑134 (MQ=255)
                                                                                                  cTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATg                                                            <  6:122196/134‑1 (MQ=255)
                                                                                                  cTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGa                                             >  7:274057/1‑149 (MQ=255)
                                                                                                    ggTTCAACGCCACGTTCAGGATCACCCGC‑AGTAGTTCGACCATCTGCTGGTGGTCACGGTCATGGGTCATAACCCCGTAACCGCCTACGGCAATCGAAGTCATAGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTTGCCATc                                           >  7:190341/1‑149 (MQ=255)
                                                                                                        cAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCcgc                                       >  4:77637/1‑149 (MQ=255)
                                                                                                         aaCGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCcgc                                       <  4:149152/148‑1 (MQ=255)
                                                                                                          aCGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGt                                     <  2:26787/149‑1 (MQ=255)
                                                                                                           cGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTg                                    <  6:332830/149‑1 (MQ=255)
                                                                                                                 gTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGc                                >  1:184170/1‑147 (MQ=255)
                                                                                                                   tCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCTTGGCGCCacc                            >  5:111533/1‑147 (MQ=255)
                                                                                                                      tgaccACCCGC‑AGATGGTCGAACAACTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTg                         <  8:249516/145‑1 (MQ=255)
                                                                                                                       gATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCAcc                                                                            <  3:247523/97‑1 (MQ=255)
                                                                                                                       gATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCAcc                                                                            >  4:247523/1‑97 (MQ=255)
                                                                                                                       gATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTgg                        >  8:186319/1‑149 (MQ=255)
                                                                                                                         tCACGCTC‑AGTTGTTCTACCATCTGCGTTTGGTCACGGTCATGGGTCATCAGCCCTTATCCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACATTGCCCACTGCAGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGgc                       <  8:190341/148‑1 (MQ=255)
                                                                                                                         tCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGgcg                      <  4:112050/149‑1 (MQ=255)
                                                                                                                          cACCCGC‑AGATGGTCGAACAACTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGgc                       >  2:90715/1‑147 (MQ=255)
                                                                                                                          cACCCGC‑AGATGGTCGAACAACTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGgc                       <  1:90715/147‑1 (MQ=255)
                                                                                                                           aCCCGC‑AGATGGTCGAACAACTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGgcgc                     >  1:41304/1‑148 (MQ=255)
                                                                                                                            cccGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGTCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGcc                    >  6:147197/1‑148 (MQ=255)
                                                                                                                            cccGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGcc                    >  2:149325/1‑148 (MQ=255)
                                                                                                                             ccGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGcc                    >  1:106070/1‑147 (MQ=255)
                                                                                                                             ccGC‑AGATGGTCGAACAACTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCCCTGGCGATCCCGCGGGGGGCCAGGGTGGCGcc                    >  4:255232/1‑147 (MQ=255)
                                                                                                                               gC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGc                <  8:105816/149‑1 (MQ=255)
                                                                                                                                      gttCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTc           <  4:275051/148‑1 (MQ=255)
                                                                                                                                         cGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTCGCt        <  2:106070/148‑1 (MQ=255)
                                                                                                                                          gACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCtttt            <  2:215752/143‑1 (MQ=255)
                                                                                                                                          gACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCtttt            >  1:215752/1‑143 (MQ=255)
                                                                                                                                          gACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTCGCTgg      <  5:291128/149‑1 (MQ=255)
                                                                                                                                          gACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTCGCTg       <  1:187864/148‑1 (MQ=255)
                                                                                                                                            ccATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTCGCTGGcc    <  4:34306/149‑1 (MQ=255)
                                                                                                                                              aTCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTCGCTGGCCTg  >  4:138098/1‑149 (MQ=255)
                                                                                                                                               tCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATccc                                         <  3:310509/109‑1 (MQ=255)
                                                                                                                                               tCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATccc                                         >  4:310509/1‑109 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GGCGCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGC‑AGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTCGCTGGCCTG  >  NC_002947/2568562‑2568851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑