Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,657,805	G→T	7.8%	intergenic (‑194/+15)	pabB ← / ← PP_2330	aminodeoxychorismate synthase/para‑aminobenzoate synthase multi‑enzyme complex/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,657,805	0	G	T	7.8%	225.9 / 5.6	72	intergenic (‑194/+15)	pabB/PP_2330	aminodeoxychorismate synthase/para‑aminobenzoate synthase multi‑enzyme complex/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (36/30);  new base T (3/3);  total (39/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGAAAAGCGCCTAGCGCTGGGTACGACATTCAACATTGTGTTTGGCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTGCGCCTCGATGGCATGGGTGCGCTGAGTCAGGC  >  NC_002947/2657661‑2657947                                                                                                                                                 |                                                                                                                                                aGGAAAAGCGCCTAGCGCTGGGTACGACATTCAACATTGTGTTTGGCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAg                                                                                                                                             >  8:250546/1‑149 (MQ=255)   gAAAAGCGCCTAGCGCTGGGTACGACATTCAACATTGTGTTTGGCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGcc                                                                                                                                           >  3:176312/1‑149 (MQ=255)     aaaGCGCCTAGCGCTGGGTACGACATTCAACATTGTGTTTGGCCCATACGCAACACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGcccc                                                                                                                                         >  1:275252/1‑149 (MQ=255)            cTAGCGCTGGGTACGACATTCAACATTGTGTTTGGCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTAttt                                                                                                                                  >  8:197291/1‑149 (MQ=255)               gcgcTGGGTACGACATTCAACATTGTGTTTGGCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCa                                                                                                                                <  4:180358/148‑1 (MQ=255)               gcgcTGGGTACGACATTCAACATTGTGTTTGGCCCATACGCAGCACAAGGCAGTTCCTGAAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑g                                                                                                                              >  2:322938/1‑149 (MQ=255)                  cTGGGTACGACATTCAACATTGTGTTTGGCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCgg                                                                                                                           >  6:280259/1‑149 (MQ=255)                       tACGACATTCAACATTGTGTTTGGCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTggg                                                                                                                      >  3:106344/1‑149 (MQ=255)                         cGACATTCAACATTGTGTTTGGCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGca                                                                                                                    <  4:176312/149‑1 (MQ=255)                         cGACATTCAACATTGTGTTTGGCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGc                                                                                                                     <  8:294828/148‑1 (MQ=255)                                 aaCATTGTGTTTGGCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGccc                                                                                                             >  7:236148/1‑148 (MQ=255)                                  aCATTGTGTTTGGCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTggg                                                                                                                      <  1:292766/138‑1 (MQ=255)                                  aCATTGTGTTTGGCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTggg                                                                                                                      >  2:292766/1‑138 (MQ=255)                                      tgtgTTTGGCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGaa                                                                                                          >  4:243244/1‑146 (MQ=255)                                          tttGGCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCt                                                                                                                                        <  4:217053/113‑1 (MQ=255)                                          tttGGCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCt                                                                                                                                        >  3:217053/1‑113 (MQ=255)                                          tttGGCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGAccc                                                                                                   <  3:243244/149‑1 (MQ=255)                                             ggCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTgg                                                                                                                       <  8:12027/126‑1 (MQ=255)                                             ggCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGAAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGGATTTAA‑GCGGTTgg                                                                                                                       >  7:12027/1‑126 (MQ=255)                                              gCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCtgtg                                                                                               <  7:250546/149‑1 (MQ=255)                                                ccATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGc                                                                                              >  4:150299/1‑148 (MQ=255)                                                  aTACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCtac                                                                                           <  7:265597/149‑1 (MQ=255)                                                      gcagcaCAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCtacccg                                                                                        >  7:129282/1‑148 (MQ=255)                                                        agcaCAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACa                                                                                                        <  1:105184/130‑1 (MQ=255)                                                        agcaCAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACa                                                                                                        >  2:105184/1‑130 (MQ=255)                                                          cacaAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCtacccgtaccc                                                                                   >  6:205192/1‑149 (MQ=255)                                                             aaGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCgct                                                                                >  1:323122/1‑149 (MQ=255)                                                                      ccTGCATGTCGAGCGTATCGCTTGAATCAACTGCAGTGCATTCAAGATCAAACTTGTGCTGCGTAATGGCTGCAATGCATCCCCTGTATTTCATCCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGc                                                                         <  1:274200/148‑1 (MQ=255)                                                                       cTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTc                                                                      >  2:308313/1‑149 (MQ=255)                                                                       cTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTc                                                                      >  2:126328/1‑149 (MQ=255)                                                                           aGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCg                                                                  <  2:323122/149‑1 (MQ=255)                                                                           aGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCg                                                                  <  2:275252/149‑1 (MQ=255)                                                                            ggTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTTTGCTACCCGTACCCGCTGCTCGGCGTCTTCgg                                                                 <  4:106344/149‑1 (MQ=255)                                                                             gTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGt                                                                >  4:161793/1‑149 (MQ=255)                                                                             gTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGt                                                                >  7:86074/1‑149 (MQ=255)                                                                             gTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGt                                                                <  8:129282/149‑1 (MQ=255)                                                                              tCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCt                                                                                                                                        <  3:207212/77‑1 (MQ=255)                                                                              tCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCt                                                                                                                                        >  4:207212/1‑77 (MQ=255)                                                                                gAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTcac                                                             <  5:280259/149‑1 (MQ=255)                                                                                    gAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACAccc                                                         <  1:126328/149‑1 (MQ=255)                                                                                         gcTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCtt                                                    <  3:161793/149‑1 (MQ=255)                                                                                             gAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGc                                                 >  2:146076/1‑148 (MQ=255)                                                                                               aTCAAATGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAg                                              >  1:301227/1‑149 (MQ=255)                                                                                                tCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCtac                                                                                           >  5:340921/1‑103 (MQ=255)                                                                                                tCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCtac                                                                                           <  6:340921/103‑1 (MQ=255)                                                                                                 cAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGCCCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACCCCCTCCTTGGCCAGTg                                            >  6:219106/1‑149 (MQ=255)                                                                                                       cAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTc                                        <  6:323691/147‑1 (MQ=255)                                                                                                       cAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCg                                       <  8:86074/148‑1 (MQ=255)                                                                                                          tGCTTGTAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCtg                                                                                                 >  7:15845/1‑87 (MQ=255)                                                                                                          tGCTTGTAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCtg                                                                                                 <  8:15845/87‑1 (MQ=255)                                                                                                            cTTGCAAGAGCAACCTTGTGCTGCTAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTgc                                 <  1:322938/149‑1 (MQ=255)                                                                                                            cTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGAtgtg                                  <  7:197291/148‑1 (MQ=255)                                                                                                            cTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTgc                                 <  1:141763/149‑1 (MQ=255)                                                                                                            cTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTgc                                 <  1:146076/149‑1 (MQ=255)                                                                                                            cTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTgc                                 <  8:236148/149‑1 (MQ=255)                                                                                                             ttGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTgcg                                >  3:70667/1‑149 (MQ=255)                                                                                                             ttGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTgcg                                >  5:359739/1‑149 (MQ=255)                                                                                                                   gagCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACCCACTCCTTGGCCAGTGCTGCGATGTGCGCCTCGa                          >  7:89476/1‑149 (MQ=255)                                                                                                                      cAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTGCGCCTCGAt                         <  1:308313/147‑1 (MQ=255)                                                                                                                         ccTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCTGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTCTCCAGTGCTTCGATGTTCGCCTCGATGGCAt                    >  8:275033/1‑149 (MQ=255)                                                                                                                         ccTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTGCGCCTCGATGGCAt                    >  8:74301/1‑149 (MQ=255)                                                                                                                                 tgcGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTTCCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTGCGCCTCGATGGCATGGGTgcgc            >  6:72609/1‑149 (MQ=255)                                                                                                                                   cGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTGCGCCTCGATGGCATGGGTGCGCt           <  2:301227/148‑1 (MQ=255)                                                                                                                                     caggggCTGCCTTGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTGCGCCTCGATGGCATGGGTGCGCTGAg        >  3:140055/4‑149 (MQ=255)                                                                                                                                     caggggCTGCCTTGCAGCCCATGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTGCGCCTCGATGGCAt                    <  6:30242/134‑1 (MQ=255)                                                                                                                                     caggggCTGCCTTGCAGCCCATGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTGCGCCTCGATGGCAt                    >  5:30242/4‑137 (MQ=255)                                                                                                                                      aggggCTGCCTTGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTGCGCCTCGAt                         <  2:200290/129‑1 (MQ=255)                                                                                                                                      aggggCTGCCTTGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTGCGCCTCGAt                         >  1:200290/3‑131 (MQ=255)                                                                                                                                       tGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTGCGCCTCGATGGCATGGGTGCGCTGAGt       >  3:191245/1‑148 (MQ=255)                                                                                                                                           cTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTGCGCCTCGATGGCATGGGTGCGCTGAGTCAGGc  >  8:153470/1‑149 (MQ=255)                                                                                                                                              cAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTGCGCCTCGATGGCAt                    >  5:253221/1‑128 (MQ=255)                                                                                                                                              cAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTGCGCCTCGATGGCAt                    <  6:253221/128‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                AGGAAAAGCGCCTAGCGCTGGGTACGACATTCAACATTGTGTTTGGCCCATACGCAGCACAAGGCAGTTCCTGCAGGTCGAGCGAAGCGCTTGAATCAACTGCAGTGCTTGCAAGAGCAACCTTGTGCTGCGAATGGGCTGCAAGGCAGCCCCTGTATTTCA‑GCGGTTGGGCACATGCCCGAACAGACCCTGTGCTACCCGTACCCGCTGCTCGGCGTCTTCGGTCACACCCTCCTTGGCCAGTGCTTCGATGTGCGCCTCGATGGCATGGGTGCGCTGAGTCAGGC  >  NC_002947/2657661‑2657947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑