Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,673,182 A→C 8.3% M166L (ATG→CTG) ‡ PP_2340 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,673,1820AC 8.3% 174.5 / 5.7 60M166L (ATG→CTG) ‡PP_2340hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (30/24);  new base C (2/3);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

CCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGA  >  NC_002947/2673037‑2673319
                                                                                                                                                 |                                                                                                                                          
ccTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATgg                                                                                                                                         <  3:218026/149‑1 (MQ=255)
 cTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATggg                                                                                                                                        <  5:102977/149‑1 (MQ=255)
         cTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTCCCTGCGCGCTGCCCATGGCAtcgcgc                                                                                                                                <  2:212845/149‑1 (MQ=255)
          tATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAgcgcg                                                                                                                                 >  5:159734/1‑147 (MQ=255)
            tGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGCTGCCCATGGCATCGCGCAg                                                                                                                              <  2:105761/148‑1 (MQ=255)
                     gtTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAgcg                                                                                                                                   >  3:84920/1‑134 (MQ=255)
                     gtTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAgcg                                                                                                                                   <  4:84920/134‑1 (MQ=255)
                         ctgcCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCgg                                                                                                                >  8:62689/1‑149 (MQ=255)
                              ggAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGa                                                                                                            >  1:307963/1‑148 (MQ=255)
                               gAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCAGTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAAt                                                                                                          <  6:194168/149‑1 (MQ=255)
                               gAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAAt                                                                                                          <  6:159734/149‑1 (MQ=255)
                                    ccGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTc                                                                                                     >  3:48892/1‑149 (MQ=255)
                                          cTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGgt                                                                                               <  8:302750/149‑1 (MQ=255)
                                           tGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCCGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGgtg                                                                                              >  3:279494/1‑149 (MQ=255)
                                           tGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGcc                                                                                                                          <  7:181538/121‑1 (MQ=255)
                                           tGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGcc                                                                                                                          >  8:181538/1‑121 (MQ=255)
                                                    ccccAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCaa                                                                                     >  7:175979/1‑149 (MQ=255)
                                                    ccccAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCaa                                                                                     >  5:337506/1‑149 (MQ=255)
                                                      ccAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAAcc                                                                                   >  7:31122/1‑149 (MQ=255)
                                                                    cgatcgatCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGttt                                                                     >  3:55154/1‑149 (MQ=255)
                                                                        cgatcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCGTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGt                                                                  <  8:31122/148‑1 (MQ=255)
                                                                         gatcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTgg                                                                <  4:48892/149‑1 (MQ=255)
                                                                          atcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGa                                                               >  3:255790/1‑149 (MQ=255)
                                                                           tcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGAt                                                              >  2:82911/1‑149 (MQ=255)
                                                                             gcgAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATg                                                             <  7:62689/148‑1 (MQ=255)
                                                                                 gTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAAc                                                       <  6:337506/149‑1 (MQ=255)
                                                                                      tcCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTg                                                   >  1:16665/1‑148 (MQ=255)
                                                                                       ccTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCGGAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATg                                                             <  6:112270/138‑1 (MQ=255)
                                                                                       ccTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTg                                                   <  8:175979/147‑1 (MQ=255)
                                                                                       ccTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCAGCGCGATGCCATCGGCAGCGCGCACGGCCAGTGACCCGGGCGAATCAGAAGCCGGTGTATTCTCAACCGCTGGTGCAGGTTTCGAGGATGCAGAAccg                                                     >  5:112270/1‑145 (MQ=255)
                                                                                          gCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAg                                              >  6:69793/1‑149 (MQ=255)
                                                                                            tttCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGa                                                 >  4:307867/1‑144 (MQ=255)
                                                                                            tttCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGa                                                 <  3:307867/144‑1 (MQ=255)
                                                                                              tCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTcc                                          >  6:122647/1‑149 (MQ=255)
                                                                                                      gcACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAg                                  >  3:166320/1‑149 (MQ=255)
                                                                                                       cACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCTGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGc                                 <  2:307963/149‑1 (MQ=255)
                                                                                                         cTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGCgg                               >  2:189171/1‑149 (MQ=255)
                                                                                                         cTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGCgg                               <  4:55154/149‑1 (MQ=255)
                                                                                                          tACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCa                                                                          <  3:176682/106‑1 (MQ=255)
                                                                                                          tACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCa                                                                          >  4:176682/1‑106 (MQ=255)
                                                                                                            ccAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCGGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGCGGCtt                            >  7:240485/1‑149 (MQ=255)
                                                                                                            ccAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGCGGc                              <  6:133579/147‑1 (MQ=255)
                                                                                                              aGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGCGGCTTGa                          >  7:17528/1‑149 (MQ=255)
                                                                                                               gcgcTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGCGGCTTGAc                         >  2:52239/1‑149 (MQ=255)
                                                                                                                cgcTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGCGGCTTGAcc                        >  3:214542/1‑149 (MQ=255)
                                                                                                                       tCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTcc                  <  5:69793/148‑1 (MQ=255)
                                                                                                                         gCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAg               <  5:122647/149‑1 (MQ=255)
                                                                                                                          cccccAGCTTCCTGCGCGATGCCATGGGGGGCGCGCAGGGCCGGTGAGCCGGGCGCATCAGTCGGCGGTGTATTCGCAACCGCTGGTGGAGGTTTCGTGGATGC‑GGGCGTTGGACAGCTCCGGCAGCAGCGGCTTGACCTGGtgc                  >  4:26305/1‑143 (MQ=255)
                                                                                                                          cccccAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGa              <  4:255790/149‑1 (MQ=255)
                                                                                                                              cAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCa          >  1:21670/1‑149 (MQ=255)
                                                                                                                                tggcccTGCGCGCTGCCCATGGCATCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCAt                                     <  7:143944/116‑1 (MQ=38)
                                                                                                                                tggcccTGCGCGCTGCCCATGGCATCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCAt                                     >  8:143944/5‑120 (MQ=38)
                                                                                                                                 cTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACtt       <  1:52239/149‑1 (MQ=255)
                                                                                                                                     cTGCGCGCTGCCCATGGCATCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGc    >  7:282025/1‑148 (MQ=255)
                                                                                                                                      tGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCg                                         >  3:3508/1‑110 (MQ=255)
                                                                                                                                      tGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGa  >  4:176304/1‑149 (MQ=255)
                                                                                                                                      tGCGCCATGCCTGGGGCCGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCg                                         <  4:3508/110‑1 (MQ=255)
                                                                                                                                        cgcgATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGa  <  2:16665/147‑1 (MQ=255)
                                                                                                                                                cATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTgg     >  3:275685/1‑136 (MQ=255)
                                                                                                                                                cATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTgg     <  4:275685/136‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                          
CCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGC‑GAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGA  >  NC_002947/2673037‑2673319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑