Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,680,461 G→C 13.1% A265G (GCC→GGC)  PP_2350 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,680,4610GC13.1% 113.6 / 10.3 46A265G (GCC→GGC) PP_2350LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (16/24);  new base C (3/3);  total (19/27)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01

AGCTGGTGGGGGTGATCACCCAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGCACCACCCGCGTGCGGAAGTAGCCACTGCCACCTTG  >  NC_002947/2680314‑2680609
                                                                                                                                                    |                                                                                                                                                    
aGCTGGTGGGGGTGATCACCCAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGc                                                                                                                                                     <  5:127577/149‑1 (MQ=255)
          ggTGATCACCCAGACCGACGTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAgg                                                                                                                                           <  6:286482/149‑1 (MQ=255)
                cACCCAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGCCCTGCAACAGGCCTTCg                                                                                                                                     <  7:235899/149‑1 (MQ=255)
                 aCCCAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACGAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGTATCGCGAAGCCCTGCCACAGGCCTTct                                                                                                                                     >  8:235899/1‑147 (MQ=255)
                 aCCCAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGCCCTGCAACAGGCCTTCg                                                                                                                                     >  6:72955/1‑148 (MQ=255)
                 aCCCAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGCCCTGCAACAGGCCTTCg                                                                                                                                     <  5:72955/148‑1 (MQ=255)
                     aGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTc                                                                                                                                <  2:70328/149‑1 (MQ=255)
                           accTGATCGCCGGCCTCAAGCGTCAGTTGCTCGAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGtt                                                                                                                           >  1:42878/1‑149 (MQ=255)
                           accTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGttt                                                                                                                          <  7:154503/149‑1 (MQ=255)
                           accTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGttt                                                                                                                          <  6:196635/149‑1 (MQ=255)
                           accTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGttt                                                                                                                          <  5:354016/149‑1 (MQ=255)
                           accTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGttt                                                                                                                          >  5:196635/1‑149 (MQ=255)
                              tGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCg                                                                                                                       >  4:249138/1‑149 (MQ=255)
                                 tCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGGGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTgg                                                                                                                    <  1:6492/149‑1 (MQ=255)
                                   gCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTggg                                                                                                                   <  8:266435/148‑1 (MQ=255)
                                    ccGGCCTCAAGCGGCAGTTGCTC‑AGCAATATCGAGGCTTCAGAAAACCGGGTCCCAGCTGTGTGACCAGTCGATGGAGCCGGCCGCTACCACCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCGTCGCTGTCGCGTTTGCGTGGGTa                                                                                                                 <  1:155925/149‑1 (MQ=255)
                                         cTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGACGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTacac                                                                                                              >  7:214047/1‑147 (MQ=255)
                                                 tCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGACGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTc                                                                                                    <  8:214047/149‑1 (MQ=255)
                                                     ttGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGt                                                                                                <  1:210859/149‑1 (MQ=255)
                                                       gCTC‑AGCAAGATAGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCATGTTGCAGGGCTTCGCTGTCGCGTGTGCGTGGGTGCACCAGGAAGGTCGGGTAg                                                                                              >  8:165329/1‑149 (MQ=255)
                                                         tC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGCCCTGCAACAGGCCTTCg                                                                                                                                     <  7:228809/108‑1 (MQ=255)
                                                         tC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGCCCTGCAACAGGCCTTCg                                                                                                                                     >  8:228809/1‑108 (MQ=255)
                                                            atcAAGATCGAGGCTTCAGATAACCGGGTCCGAGCGTTGTGACCAGTCTCTGGCGCCTGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGGAGGGCTTCGCTGTCTCGTTTGCGTGGGTACACGAGTAAGGTCGGGTATGt                                                                                            <  2:42878/145‑1 (MQ=255)
                                                            aGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTg                                                                                           >  5:302476/1‑148 (MQ=255)
                                                              cAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACAcc                                                                                                             <  2:19964/128‑1 (MQ=255)
                                                              cAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACAcc                                                                                                             >  1:19964/1‑128 (MQ=255)
                                                                aGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTc                                                                                      <  3:249138/149‑1 (MQ=255)
                                                                            cAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCg                                                                           <  8:183605/148‑1 (MQ=255)
                                                                             aGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGt                                                                         >  6:30712/1‑149 (MQ=255)
                                                                                   ccGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGt                                                                                            <  3:349012/124‑1 (MQ=255)
                                                                                   ccGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGt                                                                                            >  4:349012/1‑124 (MQ=255)
                                                                                       gTCACAACGCTGGGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTc                                                                 <  4:62422/147‑1 (MQ=255)
                                                                                       gTCACAACGCTGGGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTc                                                                 >  3:62422/1‑147 (MQ=255)
                                                                                               gTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACAcgccg                                                       <  1:172018/149‑1 (MQ=255)
                                                                                                 tgtgACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCt                                                     >  7:6074/1‑149 (MQ=255)
                                                                                                       cAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTc                                                  >  2:214873/1‑146 (MQ=255)
                                                                                                                 cgcCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGc                                     <  8:6074/149‑1 (MQ=255)
                                                                                                                  gcCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGca                                    >  3:38480/1‑149 (MQ=255)
                                                                                                                  gcCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGca                                    >  3:244894/1‑149 (MQ=255)
                                                                                                                           tACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGTGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGCACCACCcgcg                           >  3:62467/1‑149 (MQ=255)
                                                                                                                             ccAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGCACCACCCGCGTg                         >  6:322490/1‑149 (MQ=255)
                                                                                                                                  cGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGCACCACCCGCGTGCGGaa                    <  4:38480/149‑1 (MQ=255)
                                                                                                                                  cGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGCACCACCCGCGTGCGGaa                    <  1:214873/149‑1 (MQ=255)
                                                                                                                                    gcgcAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGCACCACCCGCGTGCGGAAGt                  <  6:302476/149‑1 (MQ=255)
                                                                                                                                            tCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGCACCACCCGCGTGCGGAAGTAGCCACTg          <  4:244894/149‑1 (MQ=255)
                                                                                                                                              gcgAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGc                                     <  4:10703/120‑1 (MQ=255)
                                                                                                                                                    gCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGCACCACCCGCGTGCGGAAGTAGCCACTGCCACCTTg  <  1:263894/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGCTGGTGGGGGTGATCACCCAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGCACCACCCGCGTGCGGAAGTAGCCACTGCCACCTTG  >  NC_002947/2680314‑2680609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑