Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,680,466 T→C 13.0% Q263Q (CAA→CAG) ‡ PP_2350 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,680,4660TC13.0% 107.7 / 7.8 46Q263Q (CAA→CAG) ‡PP_2350LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (16/24);  new base C (3/3);  total (19/27)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

GGTGATCACCCAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGCACCACCCGCGTGCGGAAGTAGCCACTGCCACCTTGGTC  >  NC_002947/2680324‑2680612
                                                                                                                                               |                                                                                                                                                  
ggTGATCACCCAGACCGACGTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAgg                                                                                                                                              <  6:286482/149‑1 (MQ=255)
      cACCCAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGCCCTGCAACAGGCCTTCg                                                                                                                                        <  7:235899/149‑1 (MQ=255)
       aCCCAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACGAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGTATCGCGAAGCCCTGCCACAGGCCTTct                                                                                                                                        >  8:235899/1‑147 (MQ=255)
       aCCCAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGCCCTGCAACAGGCCTTCg                                                                                                                                        <  5:72955/148‑1 (MQ=255)
       aCCCAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGCCCTGCAACAGGCCTTCg                                                                                                                                        >  6:72955/1‑148 (MQ=255)
           aGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTc                                                                                                                                   <  2:70328/149‑1 (MQ=255)
                 accTGATCGCCGGCCTCAAGCGTCAGTTGCTCGAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGtt                                                                                                                              >  1:42878/1‑149 (MQ=255)
                 accTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGttt                                                                                                                             <  5:354016/149‑1 (MQ=255)
                 accTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGttt                                                                                                                             <  7:154503/149‑1 (MQ=255)
                 accTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGttt                                                                                                                             >  5:196635/1‑149 (MQ=255)
                 accTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGttt                                                                                                                             <  6:196635/149‑1 (MQ=255)
                    tGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCg                                                                                                                          >  4:249138/1‑149 (MQ=255)
                       tCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGGGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTgg                                                                                                                       <  1:6492/149‑1 (MQ=255)
                         gCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTggg                                                                                                                      <  8:266435/148‑1 (MQ=255)
                          ccGGCCTCAAGCGGCAGTTGCTC‑AGCAATATCGAGGCTTCAGAAAACCGGGTCCCAGCTGTGTGACCAGTCGATGGAGCCGGCCGCTACCACCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCGTCGCTGTCGCGTTTGCGTGGGTa                                                                                                                    <  1:155925/149‑1 (MQ=255)
                               cTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGACGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTacac                                                                                                                 >  7:214047/1‑147 (MQ=255)
                                       tCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGACGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTc                                                                                                       <  8:214047/149‑1 (MQ=255)
                                           ttGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGt                                                                                                   <  1:210859/149‑1 (MQ=255)
                                             gCTC‑AGCAAGATAGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCATGTTGCAGGGCTTCGCTGTCGCGTGTGCGTGGGTGCACCAGGAAGGTCGGGTAg                                                                                                 >  8:165329/1‑149 (MQ=255)
                                               tC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGCCCTGCAACAGGCCTTCg                                                                                                                                        <  7:228809/108‑1 (MQ=255)
                                               tC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGCCCTGCAACAGGCCTTCg                                                                                                                                        >  8:228809/1‑108 (MQ=255)
                                                  atcAAGATCGAGGCTTCAGATAACCGGGTCCGAGCGTTGTGACCAGTCTCTGGCGCCTGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGGAGGGCTTCGCTGTCTCGTTTGCGTGGGTACACGAGTAAGGTCGGGTATGt                                                                                               <  2:42878/145‑1 (MQ=255)
                                                  aGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTg                                                                                              >  5:302476/1‑148 (MQ=255)
                                                    cAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACAcc                                                                                                                <  2:19964/128‑1 (MQ=255)
                                                    cAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACAcc                                                                                                                >  1:19964/1‑128 (MQ=255)
                                                      aGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTc                                                                                         <  3:249138/149‑1 (MQ=255)
                                                                  cAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCg                                                                              <  8:183605/148‑1 (MQ=255)
                                                                   aGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGt                                                                            >  6:30712/1‑149 (MQ=255)
                                                                         ccGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGt                                                                                               >  4:349012/1‑124 (MQ=255)
                                                                         ccGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGt                                                                                               <  3:349012/124‑1 (MQ=255)
                                                                             gTCACAACGCTGGGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTc                                                                    <  4:62422/147‑1 (MQ=255)
                                                                             gTCACAACGCTGGGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTc                                                                    >  3:62422/1‑147 (MQ=255)
                                                                                     gTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACAcgccg                                                          <  1:172018/149‑1 (MQ=255)
                                                                                       tgtgACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCt                                                        >  7:6074/1‑149 (MQ=255)
                                                                                             cAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTc                                                     >  2:214873/1‑146 (MQ=255)
                                                                                                       cgcCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGc                                        <  8:6074/149‑1 (MQ=255)
                                                                                                        gcCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGca                                       >  3:38480/1‑149 (MQ=255)
                                                                                                        gcCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGca                                       >  3:244894/1‑149 (MQ=255)
                                                                                                                 tACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGTGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGCACCACCcgcg                              >  3:62467/1‑149 (MQ=255)
                                                                                                                   ccAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGCACCACCCGCGTg                            >  6:322490/1‑149 (MQ=255)
                                                                                                                        cGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGCACCACCCGCGTGCGGaa                       <  4:38480/149‑1 (MQ=255)
                                                                                                                        cGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGCACCACCCGCGTGCGGaa                       <  1:214873/149‑1 (MQ=255)
                                                                                                                          gcgcAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGCACCACCCGCGTGCGGAAGt                     <  6:302476/149‑1 (MQ=255)
                                                                                                                                  tCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGCACCACCCGCGTGCGGAAGTAGCCACTg             <  4:244894/149‑1 (MQ=255)
                                                                                                                                    gcgAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGc                                        <  4:10703/120‑1 (MQ=255)
                                                                                                                                          gCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGCACCACCCGCGTGCGGAAGTAGCCACTGCCACCTTg     <  1:263894/149‑1 (MQ=255)
                                                                                                                                               ttGCAGGGCTTCGCTGTCGCGTTTGCGCGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGCACCACCCGCGTGCGGAAGTAGCCACTGCCACCTTGGTc  <  5:322490/147‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
GGTGATCACCCAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTC‑AGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATAGGCCTGCACCACCCGCGTGCGGAAGTAGCCACTGCCACCTTGGTC  >  NC_002947/2680324‑2680612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑