Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,697,099 G→A 6.1% intergenic (+28/+49) PP_2363 → / ← PP_2364 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,697,0990GA 6.1% 190.7 / 2.0 65intergenic (+28/+49)PP_2363/PP_2364hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (31/30);  new base A (2/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GGCGTAGGTACCGGCACGGGTAGCCAGGTGTTCAACTATAACGCCAAGGTCTATACCGATCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGGCGC  >  NC_002947/2696952‑2697246
                                                                                                                                                   |                                                                                                                                                   
ggCGTAGGTACCGGCACGGGTAGCCAGGTGTTCAACTATAACGCCAAGGTCTATACCGATCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAgccgc                                                                                                                                                    <  2:134241/149‑1 (MQ=255)
       gTACCGGCACGGGTAGCCAGGTGTTCAACTATAACGCCAAGGTCTATACCGATCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGt                                                                                                                                             >  3:271057/1‑149 (MQ=255)
       gTACCGGCACGGGTAGCCAGGTGTTCAACTATAACGCCAAGGTCTATACCGATCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGt                                                                                                                                             >  7:190770/1‑149 (MQ=255)
             gcagtggTAGCCAGGTGTTCAACTATAACGCCAAGGTCTATACCGATCCGGCGACACCGCCTGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGAc                                                                                                                                       <  8:154118/144‑1 (MQ=255)
                       ccAGGTGTTCAACTATAACGCCAAGGTCTATACCGATCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTcc                                                                                                                             >  4:135603/1‑149 (MQ=255)
                         aGGTGTTCAACTATAACGCCAAGGTCTATACCGATCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCg                                                                                                                           >  7:140207/1‑149 (MQ=255)
                             gtTCAACTATAACGCCAAGGTCTATACCGATCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAgg                                                                                                                       >  5:144345/1‑149 (MQ=255)
                              ttCAACTATAACGCCAAGGTCTATACCGATCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAgg                                                                                                                       <  8:276761/148‑1 (MQ=255)
                                 aaCTATAACGCCAAGGTCTATACCGATCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCACCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCt                                                                                                                   >  7:208756/1‑149 (MQ=255)
                                 aaCTATAACGCCAAGGTCTATACCGATCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCACCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCt                                                                                                                   >  5:76317/1‑149 (MQ=255)
                                 aaCTATAACGCCAAGGTCTATACCGATCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCCAAGCAGCCGCCGCCTGACGCGACGGGCTGCTCCCGCAGGGGCt                                                                                                                   >  7:294485/1‑149 (MQ=255)
                                  aCTATAACGCCAAGGTCTATACCGATCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTg                                                                                                                  <  2:294009/149‑1 (MQ=255)
                                    tataACGCCAAGGTCTATACCGATCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAg                                                                                                                        <  8:125835/141‑1 (MQ=255)
                                    tataACGCCAAGGTCTATACCGATCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAg                                                                                                                        >  7:125835/1‑141 (MQ=255)
                                    tataACGCCAAGGTCTATACCGATCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTgcg                                                                                                                >  7:210412/1‑149 (MQ=255)
                                      taACGCCAAGGTCTATACCGATCAGGCGACTCCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTgcgc                                                                                                               <  2:268533/148‑1 (MQ=255)
                                        aCGCCAAGGTCTATACCGATCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCaa                                                                                                             <  4:271057/148‑1 (MQ=255)
                                         cGCCAAGGTCTATACCGATCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGAGGTCAAAACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTCCGCAAgg                                                                                                           >  1:23899/1‑149 (MQ=255)
                                         cGCCAAGGTCTATACCGATCAGGCGACACCACCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAgg                                                                                                           <  5:131034/149‑1 (MQ=255)
                                          gCCAAGGTCTATACCGATCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGc                                                                                                          <  4:124451/149‑1 (MQ=255)
                                                 tCTATACCGATCAGGCGACCCCGCCGGCTGCGAACTACACCGACAGTGGGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGt                                                                                                                                             <  3:20494/107‑1 (MQ=255)
                                                          aTCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAAt                                                                                          >  5:17788/1‑149 (MQ=255)
                                                            cAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATcc                                                                                        >  1:66095/1‑149 (MQ=255)
                                                             aGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATcc                                                                                        <  4:297583/148‑1 (MQ=255)
                                                             aGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATcc                                                                                        <  8:210412/148‑1 (MQ=255)
                                                              ggCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAgg                                                                                                      <  6:339505/133‑1 (MQ=255)
                                                              ggCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAgg                                                                                                      >  5:339505/1‑133 (MQ=255)
                                                               gCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACg                                                                                                                                      >  4:347593/1‑100 (MQ=255)
                                                               gCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACg                                                                                                                                      <  3:347593/100‑1 (MQ=255)
                                                               gCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATcccc                                                                                      >  2:207004/1‑148 (MQ=255)
                                                                   cacCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCa                                                                                 >  4:348055/1‑149 (MQ=255)
                                                                     ccgccgGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCACCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATg                                                                               <  8:208756/149‑1 (MQ=255)
                                                                       gccgGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCt                                                                             >  3:97642/1‑149 (MQ=255)
                                                                       gccgGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCt                                                                             <  1:354102/149‑1 (MQ=255)
                                                                               cGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAgg                                                                     <  8:140207/149‑1 (MQ=255)
                                                                               cGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAg                                                                      <  2:66095/148‑1 (MQ=255)
                                                                                gAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATcc                                                                                        >  1:249622/1‑129 (MQ=255)
                                                                                gAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATcc                                                                                        <  2:249622/129‑1 (MQ=255)
                                                                                      cacCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAg                                                              <  1:207004/149‑1 (MQ=255)
                                                                                       acCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGc                                                             >  5:248491/1‑149 (MQ=255)
                                                                                         cGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAAcc                                                                                  <  1:221409/126‑1 (MQ=255)
                                                                                         cGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAAcc                                                                                  >  2:221409/1‑126 (MQ=255)
                                                                                         cGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCAc                                                           >  6:53150/1‑149 (MQ=255)
                                                                                         cGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCAc                                                           >  3:157057/1‑149 (MQ=255)
                                                                                               tgtgATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGACGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGcccc                                                     >  7:230222/1‑149 (MQ=255)
                                                                                                gtgATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCg                                                    <  4:174648/149‑1 (MQ=255)
                                                                                                    tACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCa                                                <  8:190770/149‑1 (MQ=255)
                                                                                                      cTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGt                                              >  1:153976/1‑149 (MQ=255)
                                                                                                         gATGTGCAGTTCTGAACGGCGGTCAATACGCCCGTAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCGAGTg                                             <  2:23899/147‑1 (MQ=255)
                                                                                                             tgCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTg                                        <  4:157057/148‑1 (MQ=255)
                                                                                                             tgCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTg                                        >  3:186892/1‑148 (MQ=255)
                                                                                                                  ttCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAgcgc                                  <  3:348055/149‑1 (MQ=255)
                                                                                                                      gAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGccc                              >  2:81456/1‑149 (MQ=255)
                                                                                                                       aaCGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAgcgc                                  >  7:21045/1‑144 (MQ=255)
                                                                                                                       aaCGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAgcgc                                  <  8:21045/144‑1 (MQ=255)
                                                                                                                          ggcggTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGa                          <  1:277584/149‑1 (MQ=255)
                                                                                                                           gcggTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGac                         <  3:135603/149‑1 (MQ=255)
                                                                                                                             ggTCAATACCCCCGAAGCAGCCGCGGCCTGTCGCGACGGTCTGCTCCCGGAGGGGCTGCGCAATGCGAGGTTCAGCGCAAATCCCCATCCATGCTGGCCAAGGTCTCGATCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGacca                       <  1:251454/149‑1 (MQ=255)
                                                                                                                                cAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCAccc                    >  4:143254/1‑149 (MQ=255)
                                                                                                                                cAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCAccc                    >  8:325361/1‑149 (MQ=255)
                                                                                                                                 aaTACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCg                   >  2:311587/1‑149 (MQ=255)
                                                                                                                                 aaTACCCCCGAAGCACCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCg                   <  8:294485/149‑1 (MQ=255)
                                                                                                                                       cccGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACg             <  5:53150/149‑1 (MQ=255)
                                                                                                                                        ccGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACgg            >  1:273465/1‑149 (MQ=255)
                                                                                                                                              cagcCACCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCgg      <  6:76317/149‑1 (MQ=255)
                                                                                                                                               agcCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGCGATGCGCAAGGCGAGGTTCAGCGAAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCggg     >  6:223090/1‑149 (MQ=255)
                                                                                                                                                gccgccgccTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGgc    <  4:97642/149‑1 (MQ=255)
                                                                                                                                                  cgccgccTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGgcgc  >  3:353337/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGCGTAGGTACCGGCACGGGTAGCCAGGTGTTCAACTATAACGCCAAGGTCTATACCGATCAGGCGACACCGCCGGCTGCGAACTACACCGACAGTGTGATACTGGATGTGCAGTTCTGAACGGCGGTCAATACCCCCGAAGCAGCCGCCGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGGCGC  >  NC_002947/2696952‑2697246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑