Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,742,867 C→T 6.8% A185V (GCG→GTG)  PP_2396 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,742,8670CT 6.8% 172.7 / 2.9 59A185V (GCG→GTG) PP_2396hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (27/28);  new base T (2/2);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

ACCGGCGAGGCCATCGACCCGCAGGTGATCCTCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGGGTGGTGTGTATCGCACTGTATCTGCCTGTGCTGCAGACCTACTGCCATACAAAAGCGCGCCTGGCACGATACATCCC  >  NC_002947/2742719‑2743014
                                                                                                                                                    |                                                                                                                                                   
acCGGCGAGGCCATCGACCCGCAGGTGATCCTCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGc                                                                                                                                                     >  8:86585/1‑149 (MQ=255)
acCGGCGAGGCCATCGACCCGCAGGTGATCCTCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGCAGCGGCGGCCCCGCTCGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGc                                                                                                                                                     >  7:228589/1‑149 (MQ=255)
 ccGGCGAGGCCATCGACCCGCAGGTGATCCTCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCg                                                                                                                                                    <  4:156209/149‑1 (MQ=255)
  cGGCGAGGCCATCGACCCGCAGGTGATCCTCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCgg                                                                                                                                                   >  1:151376/1‑149 (MQ=255)
  cGGCGAGGCCATCGACCCGCAGGTGATCCTCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCgg                                                                                                                                                   >  3:309905/1‑149 (MQ=255)
           cATCGACCCGCAGGTGATCCTCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGccc                                                                                                                                           >  8:151168/1‑148 (MQ=255)
             tCGACCCGCAGGTGATCCTCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCaaa                                                                                                                                        >  2:145035/1‑149 (MQ=255)
                 cccGCAGGTGATCCTCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGc                                                                                                                                    <  2:151376/149‑1 (MQ=255)
                  ccGCAGGTGATCCTCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCt                                                                                                                                   <  5:248378/149‑1 (MQ=255)
                  ccGCAGGTGATCCTCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCt                                                                                                                                   <  7:218151/149‑1 (MQ=255)
                    gCAGGTGATCCTCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGTGGCCCCGCCCAAACCGCTg                                                                                                                                  >  5:66078/1‑148 (MQ=255)
                    gCAGGTGATCCTCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGTGGCCCCGCCCAAACCGCTg                                                                                                                                  >  5:71192/1‑148 (MQ=255)
                    gCAGGTGATCCTCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTg                                                                                                                                  >  4:307506/1‑148 (MQ=255)
                     cAGGTGATCCTCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGa                                                                                                                                <  1:145035/149‑1 (MQ=255)
                       ggTGATCCTCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCg                                                                                                                                                    <  2:100353/127‑1 (MQ=255)
                       ggTGATCCTCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCg                                                                                                                                                    >  1:100353/1‑127 (MQ=255)
                            tCCTCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTg                                                                                                                          >  7:129602/1‑148 (MQ=255)
                               tCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCa                                                                                                                      >  4:307895/1‑149 (MQ=255)
                                         cGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATgg                                                                                                            <  7:86585/149‑1 (MQ=255)
                                          gatgatCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGc                                                                                                           <  3:307506/149‑1 (MQ=255)
                                                   cctgccAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCaa                                                                                                      <  3:307895/145‑1 (MQ=255)
                                                   cctgccAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCa                                                                                                  >  4:217259/1‑149 (MQ=255)
                                                       ccAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCt                                                                                               <  1:253752/148‑1 (MQ=255)
                                                         aGCGAGAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTgg                                                                                            >  7:326177/1‑149 (MQ=255)
                                                         aGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTgg                                                                                            <  4:309905/149‑1 (MQ=255)
                                                         aGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTgg                                                                                            <  3:217259/149‑1 (MQ=255)
                                                           cGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGTGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGTGCCTTgggg                                                                                          <  6:71192/149‑1 (MQ=255)
                                                           cGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGTGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGTGCCTTgggg                                                                                          <  6:66078/149‑1 (MQ=255)
                                                           cGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGTGCCTTgggg                                                                                          <  5:335310/149‑1 (MQ=255)
                                                            gAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGTGCCTTGGGGc                                                                                         <  7:151168/149‑1 (MQ=255)
                                                             aaaaGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGcc                                                                                        >  8:99167/1‑149 (MQ=255)
                                                              aaaGGCCGGGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAACACGACAGCACGCCGGCCAAGCCCGCCAAGCCAGCTGCCCCGCCCAAACCGCTGGAAGAATGATCACGCCTCATTGCCACAAGGCACCTTGGGGccc                                                                                       <  8:134565/149‑1 (MQ=255)
                                                                  gCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTg                                                                                   >  4:255236/1‑149 (MQ=255)
                                                                    ggTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGcc                                                                                 <  5:76259/149‑1 (MQ=255)
                                                                     gTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCg                                                                                <  5:296578/149‑1 (MQ=255)
                                                                         cggcACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGggtg                                                                            <  3:255236/149‑1 (MQ=255)
                                                                          ggcACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGggtgg                                                                           >  2:350918/1‑149 (MQ=255)
                                                                               ccGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTg                                                                                                                                  >  6:20601/1‑89 (MQ=255)
                                                                               ccGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTg                                                                                                                                  <  5:20601/89‑1 (MQ=255)
                                                                               ccGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGAt                                                                                                                        <  3:23605/99‑1 (MQ=255)
                                                                               ccGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGAt                                                                                                                        >  4:23605/1‑99 (MQ=255)
                                                                                cGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGGGTGGTGTGTa                                                                     >  5:266052/1‑149 (MQ=255)
                                                                                     ccGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGGGTGGTGTGTATCGCa                                                                <  1:350918/149‑1 (MQ=255)
                                                                                          gcggcgGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGGGTGGTGTGTATCGCACTGt                                                            <  8:129602/148‑1 (MQ=255)
                                                                                          gcggcgGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGGGTGGTGTGTATCGCACTGTa                                                           >  1:351553/1‑149 (MQ=255)
                                                                                                         gCCAAGGAACAGGCCCGCAAGCCGGCCACGCCCGCCAAGCCAGCGGCCCCGCCCAAACCCCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGGGTGGTGTGTATCGCACTGTATCTGCCTGTGCTGCa                                            <  8:228589/149‑1 (MQ=255)
                                                                                                          ccAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGggtggt                                                                          <  4:229801/118‑1 (MQ=255)
                                                                                                          ccAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGggtggt                                                                          >  3:229801/1‑118 (MQ=255)
                                                                                                                   aGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGGGTGGTGTGTATCGCACTGTATCTGCCTGTGCTGCAGACCTACTGc                                  >  4:90148/1‑149 (MQ=255)
                                                                                                                        aGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGGGTGGTGTGTATCGCACTGTATCTGCCTGTGCTGCAGACCTACTGCCATa                              >  7:31220/1‑148 (MQ=255)
                                                                                                                          cAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGc                                                                                  <  3:254524/94‑1 (MQ=255)
                                                                                                                          cAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGc                                                                                  >  4:254524/1‑94 (MQ=255)
                                                                                                                            aGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGCCTGAACACGCCTCATGGCCCCAAGGCACCATGGCGCCCGTTGCCGGGTGGTGTGTATCGCACTGTATCTGCCTGTGCTGCAGACCTACTGCCAGACaaac                         >  7:209749/1‑148 (MQ=255)
                                                                                                                            aGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGGGTGGTGTGTATCGCACTGTATCTGCCTGTGCTGCAGACCTACTGCCATACaa                           >  2:289281/1‑147 (MQ=255)
                                                                                                                                 gCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGGGTGGTGTGTATCGCACTGTATCTGCCTGTGCTGCAGACCTACTGCCATACAAAAgcgcg                    >  8:179174/1‑149 (MQ=255)
                                                                                                                                  ccAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGGGTGGTGTGTATCGCACTGTATCTGCCTGTGCTGCAGACCTACTGCCATACAAAAgcgcgc                   >  6:135834/1‑149 (MQ=255)
                                                                                                                                      gCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGGGTGGTGTGTATCGCACTGTATCTGCCTGTGCTGCAGACCTACTGCCATACAAAAgcgcg                    >  3:268075/1‑144 (MQ=255)
                                                                                                                                      gCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGGGTGGTGTGTATCGCACTGTATCTGCCTGTGCTGCAGACCTACTGCCATACAAAAgcgcg                    <  4:268075/144‑1 (MQ=255)
                                                                                                                                         cGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGGGTGGTGTGTATCGCACTGTATCTGCCTGTGCTGCAGACCTACTGCCATACAAAAGCGCGCCTGGCAc            <  6:266052/149‑1 (MQ=255)
                                                                                                                                                 cAGCGGCCCCGCCAAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGGGTGGTGTGTATCGCACTGTATCTGCCTGTGCTGCAGACCTACTGCCATACAAAAGCGCGCCTGGCACGATACATc    >  5:199215/1‑149 (MQ=255)
                                                                                                                                                   gCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGGGTGGTGTGTATCGCACTGTATCTGCCTGTGCTGCAGACCTACTGCCATACAAAAGCGCGCCTGGCACGATACATccc  <  2:351553/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACCGGCGAGGCCATCGACCCGCAGGTGATCCTCAAGGCGGTCGATGATCTGCCTGCCAGCGAAAAGGCGGTGGCGGCACCCGAGCCCGTAGCGGCGGCCCCGCAAGCCAAGGAAAAGGACAGCAAGCCGGCCAAGCCCGCCAAGCCAGCGGCCCCGCCCAAACCGCTGGAAGACTGATCACGCCTCATGGCCCCAAGGCACCTTGGGGCCCGTTGCCGGGTGGTGTGTATCGCACTGTATCTGCCTGTGCTGCAGACCTACTGCCATACAAAAGCGCGCCTGGCACGATACATCCC  >  NC_002947/2742719‑2743014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑