Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,782,481 A→C 7.0% intergenic (‑116/+15) iscS‑II ← / ← PP_2436 cysteine desulfurase/LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,782,4810AC 7.0% 171.3 / 4.5 57intergenic (‑116/+15)iscS‑II/PP_2436cysteine desulfurase/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (26/27);  new base C (2/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

GGCGATCATTGACGTAAGCGTACCTGCCAACAGCGGTATTTTTTCGTTTCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGTTGGCCGGGTCCTGGTCGGTGCTTTCATGCCAGTACAGGTGCGTTTCCAGT  >  NC_002947/2782336‑2782625
                                                                                                                                                 |                                                                                                                                                  
ggCGATCATTGACGTAAGCGTACCTGCCAACAGCGGTATTTTTTCGTTTCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCt                                                                                                                                                 >  4:126106/1‑149 (MQ=255)
   gATCATTGACGTAAGCGTACCTGCCAACAGCGGTATTTTTTCGTTTCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCAc                                                                                                                                              <  6:199929/149‑1 (MQ=255)
     tCATTGACGTAAGCGTACCTGCCAACAGCGGTATTTTTTCGTTTCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGTGCCATTAATAGCCCCTCAcca                                                                                                                                            <  1:170773/149‑2 (MQ=255)
          gACGTAAGCGTACCTGCCAACAGCGGTATTTTTTCGTTTCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCAc                                                                                                                                        <  7:123246/148‑1 (MQ=255)
             gTAAGCGTACCTGCGAACAGCGGTATTTTTTCGTTTCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTTATGGCACCTCACCGGCACCTTc                                                                                                                                    >  4:36952/1‑149 (MQ=255)
             gTAAGCGTACCTGCCAACAGCGGTATTTTTTCGTTTCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCtt                                                                                                                                     >  2:271530/1‑148 (MQ=255)
                aGCGTACCTGCCAACAGCTGTATTTTTTCGTTTCCCTCAGGTCTATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAg                                                                                                                                  <  3:316573/148‑1 (MQ=255)
                 gCGTACCTGCCAACAGCGGTATTTTTTCGTTTCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGc                                                                                                                                >  5:352215/1‑149 (MQ=255)
                 gCGTACCTGCCAACAGCGGTATTTTTTCGTTTCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGc                                                                                                                                >  7:2117/1‑149 (MQ=255)
                    tACCTGCCAACAGCGGTATTTTTTCGTTTCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTg                                                                                                                             <  2:299823/149‑1 (MQ=255)
                      ccTGCCAACAGCGGTATTTTTTCGTTTCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGc                                                                                                                            <  1:176793/148‑1 (MQ=255)
                               aGCGGTATTTTTTCGTTTCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCt                                                                                                                >  8:168143/1‑149 (MQ=255)
                                   gTATTTTTTCGTTTCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAAGTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTttc                                                                                                            <  7:256629/149‑1 (MQ=255)
                                   gTATTTTTTCGTTTCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTttc                                                                                                            <  3:126106/149‑1 (MQ=255)
                                         tttCGTTTCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTttc                                                                                                            >  4:143062/1‑143 (MQ=255)
                                         tttCGTTTCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTttc                                                                                                            <  3:143062/143‑1 (MQ=255)
                                         tttCGTTTCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCctc                                                                                                      >  4:116772/1‑149 (MQ=255)
                                             gTTTCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTACAGGCGTGCTc                                                                                                                          >  1:193794/1‑127 (MQ=255)
                                             gTTTCCCTAATGTCGATTCGAGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTc                                                                                                                          <  2:193794/127‑1 (MQ=255)
                                              tttCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGcca                                                                                                 >  4:290546/1‑149 (MQ=255)
                                                  ccTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCcaccac                                                                                             <  3:116772/149‑1 (MQ=255)
                                                           gATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAg                                                                                                                                  >  5:205288/1‑105 (MQ=255)
                                                           gATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAg                                                                                                                                  <  6:205288/105‑1 (MQ=255)
                                                               cgcgATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCtttt                                                                                                             <  6:309439/120‑1 (MQ=255)
                                                               cgcgATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCtttt                                                                                                             >  5:309439/1‑120 (MQ=255)
                                                                   attattTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCgg                                                                            >  6:149935/1‑149 (MQ=255)
                                                                   attattTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAAGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCgg                                                                            <  1:274085/149‑1 (MQ=255)
                                                                       ttttAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGAt                                                                        >  1:101972/1‑149 (MQ=255)
                                                                          tAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GCGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTg                                                                     >  8:122713/1‑149 (MQ=255)
                                                                               aaaaaTTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCATTGCGGATTTTATTCATATACCACAACCCCATCGAACAATACGTTGATCTGTTc                                                                  >  1:296759/1‑149 (MQ=255)
                                                                               aaaaaTTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTcgc                                                                <  2:101972/149‑1 (MQ=255)
                                                                                aaaaTTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGAt                                                                        <  2:32929/140‑1 (MQ=255)
                                                                                aaaaTTCTCTATCGAAGAACTTTCATGGAAATTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGAt                                                                        >  1:32929/1‑140 (MQ=255)
                                                                                         tATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAg                                                        <  6:352215/147‑1 (MQ=255)
                                                                                          aTCGAAGAACTTTCATGGAGTTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCACGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCgg                                                     <  6:306821/149‑1 (MQ=255)
                                                                                             gaagaaCTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGtt                                                   <  3:208058/148‑1 (MQ=255)
                                                                                             gaagaaCTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGTTg                                                  >  6:320008/1‑149 (MQ=255)
                                                                                              aagaaCTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGTTgg                                                 >  7:249207/1‑149 (MQ=255)
                                                                                              aagaaCTTTCATGGAATTTTCTACTTCGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGTTgg                                                 >  5:93967/1‑149 (MQ=255)
                                                                                                         tGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTTATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGTTGGCCGGGTCCTgg                                      >  2:138691/1‑149 (MQ=255)
                                                                                                         tGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGTTGGCCGGGTCCTgg                                      <  7:168143/149‑1 (MQ=255)
                                                                                                             aTTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGTTGGCCGGGTCCTGGTCgg                                  <  1:271530/149‑1 (MQ=255)
                                                                                                                ttCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGTTGGCCGGGTCCTGGTCGGTg                                <  5:149935/148‑1 (MQ=255)
                                                                                                                   tACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGTTGGCCGGGTCCTGGTCGGTGCttt                            >  3:297959/1‑149 (MQ=255)
                                                                                                                     cTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGTTGGCCGGGTCCTGGTCGGTGCtt                             <  5:320008/146‑1 (MQ=255)
                                                                                                                     cTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGTTGGCCGGGTCCTGGTCGGTGCTTTCa                          <  8:307912/149‑1 (MQ=255)
                                                                                                                        tGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGTTGGCCGGGTCCTGGTCGGTGCTTTCAt                         <  8:249207/147‑1 (MQ=255)
                                                                                                                              gTGAGGTGCCATTAATAGCCCCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCg                                                                             <  7:140613/89‑1 (MQ=38)
                                                                                                                              gTGAGGTGCCATTAATAGCCCCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCg                                                                             >  8:140613/1‑89 (MQ=38)
                                                                                                                                   gtgccaTTAATAGCCCCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGTTGGCCGGGTCCTGGTCGGTGCTTTCATGCCAGTACAGGTg            >  3:160170/6‑149 (MQ=255)
                                                                                                                                      cTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAg                                                        <  3:333774/102‑1 (MQ=255)
                                                                                                                                      cTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAg                                                        >  4:333774/1‑102 (MQ=255)
                                                                                                                                      cTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGTTGGCCGGGTCCTGGTCGGTGCTTTCATGCCAGTACAGGTGCGt         >  6:245616/1‑149 (MQ=255)
                                                                                                                                       tATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGTTGGCCGGGTCCTGGTCGGTGCTTTCATGCCAGTACAGGTGCGtt        >  6:65451/1‑149 (MQ=255)
                                                                                                                                         ttAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGTTGGCCGGGTCCTGGTCGGTGCTTTCATGCCAGTACAGGTGCGTTTc      <  2:296759/149‑1 (MQ=255)
                                                                                                                                            aTGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGTTGGCCGGGTCCTGGTCGGTGCTTTCATGCCAGTACAGGTGCGTTTCCAg   >  7:208235/1‑149 (MQ=255)
                                                                                                                                             tGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGTTGGCCGGGTCCTGGTCGGTGCTTTCATGCCAGTACAGGTGCGTTTCCAGt  <  5:65451/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GGCGATCATTGACGTAAGCGTACCTGCCAACAGCGGTATTTTTTCGTTTCCCTCAGGTCGATTCGCGATTATTTTAGAAAAAAATTCTCTATCGAAGAACTTTCATGGAATTTTCTACTTTGTCTGGTGAGGGGCTATTAATGGCACCTCACCGGCACCTTCAGGCGTGCTCCA‑‑GAGCCTTTTCTTCCTCTGCCACCACGCGCTCGCACAATTCGGTGATCTGTTCGCGCATCCAGCGGTTGGCCGGGTCCTGGTCGGTGCTTTCATGCCAGTACAGGTGCGTTTCCAGT  >  NC_002947/2782336‑2782625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑