Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,886,149 A→T 7.8% M282K (ATG→AAG) ‡ PP_5536 ← conserved protein of unknown function with RHS repeat‑associated core domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,886,1490AT 7.8% 148.6 / 2.6 51M282K (ATG→AAG) ‡PP_5536conserved protein of unknown function with RHS repeat‑associated core domain
Reads supporting (aligned to +/- strand):  ref base A (23/24);  new base T (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

GAGGCCTTTGTGGGAGATCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGATTGTTCGGG  >  NC_002947/2886003‑2886296
                                                                                                                                                  |                                                                                                                                                   
gAGGCCTTTGTGGGAGATCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTAGGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCa                                                                                                                                                     >  2:86541/1‑147 (MQ=255)
   gCCTTTGTGGGAGATCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGt                                                                                                                                                >  2:37404/1‑149 (MQ=255)
   ctcTTTGTGGGAGATCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGAGTCCGAACGCTTGCGGCCTGTTCAACCATAgg                                                                                                                                                 <  1:27232/146‑1 (MQ=255)
    ccTTTGTGGGAGATCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGtt                                                                                                                                               <  3:167731/149‑1 (MQ=255)
      tttGTGGGAGATCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAgg                                                                                                                                                 >  2:27232/1‑145 (MQ=255)
              agaTCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAg                                                                                                                                                  <  1:318264/136‑1 (MQ=255)
              agaTCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAg                                                                                                                                                  >  2:318264/1‑136 (MQ=255)
               gaTCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGa                                                                                                                                      >  7:46737/1‑147 (MQ=255)
                   gCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTAGCCGGACAAAAAGGGCTGCCTTGCAGATGTTTTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAggg                                                                                                                                 <  1:189606/148‑1 (MQ=255)
                   gCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAggg                                                                                                                                 <  4:342345/148‑1 (MQ=255)
                   gCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAggg                                                                                                                                 <  8:13681/148‑1 (MQ=255)
                    cccAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGgt                                                                                                                               <  6:111047/149‑1 (MQ=255)
                     ccAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCTATGGTTGaaca                                                                                                                                          <  4:197944/137‑3 (MQ=255)
                     ccAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCTATGGTTGaaca                                                                                                                                          >  3:197944/1‑135 (MQ=255)
                         ccccTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAAt                                                                                                                          <  1:37404/149‑1 (MQ=255)
                               tGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAGGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGaa                                                                                                                      <  4:195839/147‑1 (MQ=255)
                                     cgcCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCttt                                                                                                                <  8:327657/147‑1 (MQ=255)
                                                 gagaACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGt                                                                                                  >  4:184768/1‑149 (MQ=255)
                                                        cAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCtt                                                                                                                 >  2:247306/1‑127 (MQ=255)
                                                        cAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCtt                                                                                                                 <  1:247306/127‑1 (MQ=255)
                                                         aGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGttttttt                                                                                            <  8:46737/147‑1 (MQ=255)
                                                         aGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTg                                                                                           >  1:60547/1‑148 (MQ=255)
                                                         aGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTg                                                                                           >  4:112116/1‑148 (MQ=255)
                                                                   cGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAgattttga                                                                                <  1:326363/149‑1 (MQ=255)
                                                                     gTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTtata                                                                                                       <  1:44627/124‑1 (MQ=255)
                                                                     gTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTtata                                                                                                       >  2:44627/1‑124 (MQ=255)
                                                                         gCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTtg                                                                          >  5:13640/1‑149 (MQ=255)
                                                                          cGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTtgt                                                                         <  8:149455/149‑1 (MQ=255)
                                                                          cGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTtgt                                                                         <  7:156267/149‑1 (MQ=255)
                                                                            tACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTgtgt                                                                       <  2:60547/149‑1 (MQ=255)
                                                                               ccGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTAtt                                                                    >  3:8225/1‑149 (MQ=255)
                                                                                   aCAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTta                                                                                                         <  3:40293/108‑1 (MQ=255)
                                                                                   aCAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTta                                                                                                         >  4:40293/1‑108 (MQ=255)
                                                                                   aCAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTAttgttt                                                                <  4:8225/149‑1 (MQ=255)
                                                                                        aaGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGGTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCtt                                                           <  6:242806/149‑1 (MQ=255)
                                                                                           ggCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCttgtt                                                        >  4:19188/1‑149 (MQ=255)
                                                                                                ccTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTgg                                                    <  6:13640/148‑1 (MQ=255)
                                                                                                    gCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTg                                                <  3:19188/148‑1 (MQ=255)
                                                                                                    gCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTCTTGTGGCTTg                                                <  3:112116/148‑1 (MQ=255)
                                                                                                        aTGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTAt                                           >  8:44703/1‑149 (MQ=255)
                                                                                                                   gCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACgg                                >  4:235306/1‑149 (MQ=255)
                                                                                                                           gAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCta                        >  7:206543/1‑149 (MQ=255)
                                                                                                                               gCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACg                    >  2:223714/1‑149 (MQ=255)
                                                                                                                                      gCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTAc             <  7:44703/149‑1 (MQ=255)
                                                                                                                                         gtttCAACCTATGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGAt          >  1:346095/3‑149 (MQ=255)
                                                                                                                                          tttCAACCTATGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGAtt         <  2:346095/148‑1 (MQ=255)
                                                                                                                                          gTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGAtt         >  6:196596/1‑149 (MQ=255)
                                                                                                                                           ttCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGATTg        >  5:144561/1‑149 (MQ=255)
                                                                                                                                           ttCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGATTg        >  2:220409/1‑149 (MQ=255)
                                                                                                                                           ttCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGGTTTATTGATGTGTTTTTGCAAGATTTTGATGTTTGTGTATTGTTTGTGTTGTGGTGGATTGGGTAGCGTGGTGAGGGTTCCGCTCTCCGCTGTTAGGATTg        >  8:273313/1‑149 (MQ=255)
                                                                                                                                                ccATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGATTGTTCgg   >  2:247209/1‑149 (MQ=255)
                                                                                                                                                 cATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGATTGTTCggg  >  3:104098/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GAGGCCTTTGTGGGAGATCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGCCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGATTGTTCGGG  >  NC_002947/2886003‑2886296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑