Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,897,380 G→A 6.8% T233T (ACG→ACA)  PP_2551 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,897,3800GA 6.8% 171.1 / 2.2 59T233T (ACG→ACA) PP_2551LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (24/31);  new base A (2/2);  total (26/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01

CCGCGCTCAGCCAGGCCGACTACCTGGCGTACAAGCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCT  >  NC_002947/2897235‑2897526
                                                                                                                                                 |                                                                                                                                                   
cggggcTCAGCGAGGCCGACTAGCTGGCGTAGAAGGTTGTGGGCTTTTCGGGCGGTGGCGACTAGGACAGCTTTTTCGACCGGCACTTGCGTGCAGAGGGTCTGCAGCGGCGGGTGAGCCTGGTAGTGCCGTTCTTCTGCGCCACGGTa                                                                                                                                                  <  4:242341/145‑1 (MQ=255)
 cgcgCTCAGCCAGGCCGACTACCTGGCGTACAAGCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTa                                                                                                                                                  >  6:56413/1‑148 (MQ=255)
      tCAGCCAGGCCGACTACCTGGCGTACAAGCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGt                                                                                                                                             >  4:25378/1‑148 (MQ=255)
        aGCCAGGCCGACTACCTGGCGTACAAGCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGa                                                                                                                                           <  3:63418/148‑1 (MQ=255)
        aGCCAGGCCGACTACCTGGCGTACAAGCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGa                                                                                                                                           <  3:13254/148‑1 (MQ=255)
         gtcAGGCCGACTACCTGGCGTACAAGCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATGGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGGCGTTCTTCTGCGCCACGGTACAAGTGAtt                                                                                                                                         <  2:306532/147‑1 (MQ=255)
         gCCAGGCCGACTACCTGGCGTACAAGCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGAtt                                                                                                                                         <  1:154685/149‑1 (MQ=255)
             ggCCGACTACCTGGCGTACAAGCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCca                                                                                                                                     <  5:337975/149‑1 (MQ=255)
                cGACTACCTGGCGTACAAGCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCcagca                                                                                                                                  <  5:200041/149‑1 (MQ=255)
                      ccTGGCGTACAAGCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAgg                                                                                                                           <  8:90611/149‑1 (MQ=255)
                       cTGGCGTACAAGCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGc                                                                                                                          >  7:269294/1‑149 (MQ=255)
                       cTGGCGTACAAGCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGc                                                                                                                          >  5:285782/1‑149 (MQ=255)
                        tGGCGTACAAGCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGcc                                                                                                                         >  1:6612/1‑149 (MQ=255)
                         ggCGTACAAGCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCg                                                                                                                        >  4:286805/1‑149 (MQ=255)
                               cAAGCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTg                                                                                                                      <  6:359261/145‑1 (MQ=255)
                               cAAGCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTg                                                                                                                      >  5:359261/1‑145 (MQ=255)
                                 aGCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACg                                                                                                                <  5:363650/149‑1 (MQ=255)
                                 aGCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACg                                                                                                                >  4:74365/1‑149 (MQ=255)
                                  gCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACgg                                                                                                               <  5:56413/149‑1 (MQ=255)
                                    aTGTGCGCATTTCGAGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTc                                                                                                             <  8:175070/149‑1 (MQ=255)
                                      gtgCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTccc                                                                                                           >  2:110591/1‑149 (MQ=255)
                                          gcATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAg                                                                                                       <  3:193848/149‑1 (MQ=255)
                                                             cAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGa                                                                                    <  6:285782/149‑1 (MQ=255)
                                                             cAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGa                                                                                    <  8:269294/149‑1 (MQ=255)
                                                                ggACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGcc                                                                                 >  2:238828/1‑149 (MQ=255)
                                                                    aGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGTTTGCGAGCAGG‑CAGGCCGTGGCCACGGTCCCCGAGCACTTCGCCCGGCAACTGAGCCGttt                                                                             <  2:321947/149‑1 (MQ=255)
                                                                    aGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGttt                                                                             <  1:359872/149‑1 (MQ=255)
                                                                     gCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTg                                                                            <  2:6612/149‑1 (MQ=255)
                                                                                cGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCCCGGTCCCCGAGCACATCGCCCGGCAACGGAGCCGGTTGCATGAcctggc                                                                 >  7:148661/1‑149 (MQ=255)
                                                                                cGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGAcctggc                                                                 >  3:118910/1‑149 (MQ=255)
                                                                                cGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGAcctggc                                                                 <  3:6621/149‑1 (MQ=255)
                                                                                      ttGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACctggcctggc                                                            >  5:6125/1‑148 (MQ=255)
                                                                                             cacaGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTg                                                    <  3:286805/149‑1 (MQ=255)
                                                                                                ggggCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGc                                                 >  4:29580/2‑149 (MQ=255)
                                                                                                  ggCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACAGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACctggcctggc                                                            >  4:339218/1‑136 (MQ=255)
                                                                                                  ggCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACAGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACctggcctggc                                                            >  4:193208/1‑136 (MQ=255)
                                                                                                  ggCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACAGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACctggcctggc                                                            <  3:193208/136‑1 (MQ=255)
                                                                                                  ggCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACAGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACctggcctggc                                                            <  3:339218/136‑1 (MQ=255)
                                                                                                     cTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCAt                                            >  2:77751/1‑149 (MQ=255)
                                                                                                        cAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCg                                          <  1:77751/148‑1 (MQ=255)
                                                                                                         aGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGAc                                        <  1:110591/149‑1 (MQ=255)
                                                                                                          gcggcgGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGAcc                                       <  4:118910/149‑1 (MQ=255)
                                                                                                                       cTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTa                          >  2:261610/1‑149 (MQ=255)
                                                                                                                               gCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCg                  >  5:335432/1‑149 (MQ=255)
                                                                                                                                ccGTTCTTCTGCGCCACGGTACAAGTGTTTGGCAGCAGT‑CAGGGCGTGGCCAGGGTCCGCGAGCACATCGCCCGGCTACTGAGCCGTTTGCATGACCTGGCCTTGCGACCAGTGGGCTTGATCGACCACAGCCAGCGCTAGTGGGTCgt                 <  5:118204/149‑1 (MQ=255)
                                                                                                                                 ccttcttcTGCGCCACGCTACAACTCATTGCCAGCAGA‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACAGAGCAGATTGCATGACCTGGCCTCGCGACCACTTGTCTTCATCTACCACAGCCACCTCAAGTGGGTAgtg                >  6:118204/3‑149 (MQ=255)
                                                                                                                                   ttcttcTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTg                                                    <  6:13719/111‑1 (MQ=255)
                                                                                                                                   ttcttcTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTg                                                    >  5:13719/1‑111 (MQ=255)
                                                                                                                                   ttcttcTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGtgtg              <  6:335432/149‑1 (MQ=255)
                                                                                                                                   ttcttcTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGtgtg              >  1:188832/1‑149 (MQ=255)
                                                                                                                                           cgcCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAg       <  4:20504/148‑1 (MQ=255)
                                                                                                                                           cgcCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAg       >  3:20504/1‑148 (MQ=255)
                                                                                                                                             ccACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAgcgc    <  6:6125/149‑1 (MQ=255)
                                                                                                                                              cACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCg                                          >  7:191029/1‑110 (MQ=255)
                                                                                                                                              cACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCg                                          <  8:191029/110‑1 (MQ=255)
                                                                                                                                               aCGGTACAAGTGATTGCCAGCAGTACAGGCCTTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGCCCAGTGGGCTTCATCGACCACAGCCAGCGGTACTGGGTc                   >  7:49538/1‑133 (MQ=255)
                                                                                                                                               aCGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTc                   <  8:49538/132‑1 (MQ=255)
                                                                                                                                               aCGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCt  >  7:234235/1‑149 (MQ=255)
                                                                                                                                               aCGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCt  <  3:25378/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CCGCGCTCAGCCAGGCCGACTACCTGGCGTACAAGCATGTGCGCATTTCGGGCGGTGGCGACAAGGACAGCTTCATCGACCGGCACTTGCGTGCACAGGGCCTGCAGCGGCGGGTCAGCCTGGAAGTGCCGTTCTTCTGCGCCACGGTACAAGTGATTGCCAGCAGT‑CAGGCCGTGGCCACGGTCCCCGAGCACATCGCCCGGCAACTGAGCCGTTTGCATGACCTGGCCTGGCGACCACTGGGCTTCATCGACCACAGCCAGCGCTACTGGGTCGTGTGGCACCAGCGCCT  >  NC_002947/2897235‑2897526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑