Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,958,816 G→A 8.3% intergenic (+82/+60) PP_2589 → / ← PP_2590 aldehyde dehydrogenase family protein/ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,958,8160GA 8.3% 127.4 / 2.3 48intergenic (+82/+60)PP_2589/PP_2590aldehyde dehydrogenase family protein/ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base G (26/18);  new base A (2/2);  total (28/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-01

TCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGCACACCCCAGTAGTCCTT  >  NC_002947/2958672‑2958962
                                                                                                                                                |                                                                                                                                                  
tCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGa                                                                                                                                                   <  8:72241/146‑1 (MQ=255)
tCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCt                                                                                                                                                >  5:262742/1‑149 (MQ=255)
tCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAACCCTCAGACTTGTGCTGAAc                                                                                                                                                  <  4:159999/147‑1 (MQ=38)
    tGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCAAGCCCGCTCCCACAGGATCAGCACAACGCTGAGCGTACTGCGGGACCGGTgg                                                                                                                                            >  8:132662/1‑149 (MQ=255)
            ttcgACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGgc                                                                                                                                    <  1:352254/149‑1 (MQ=255)
               gACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGgcgcg                                                                                                                                 >  7:63175/1‑149 (MQ=255)
                aCAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTgg                                                                                                                                            >  8:214004/1‑137 (MQ=255)
                aCAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTgg                                                                                                                                            <  7:214004/137‑1 (MQ=255)
                       ttCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCcgcg                                                                                                                           >  3:273588/1‑147 (MQ=255)
                         cGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGaa                                                                                                                         >  6:232388/1‑147 (MQ=255)
                            aaCTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGc                                                                                                                    >  4:62293/1‑149 (MQ=255)
                                cAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTa                                                                                                                >  3:166298/1‑149 (MQ=255)
                                         cTGGTCCCAGTTGCGCGGGTGATGTATTGATCTTTGCGCGCGTCGTCTGCTTCGGGGGCATGCCCGCTCCCAGAGGTTCAGCACAAGTCTGAGGGTTGTGGTGGACCTGTGGGAGGGGGCGCGCCCGCGAAGAGGCCGTATCTGGcgcc                                                                                                       <  4:274115/149‑1 (MQ=38)
                                              aCCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCgcggc                                                                                                  >  3:144129/1‑149 (MQ=255)
                                                      cgcgGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGGCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGAccc                                                                                           <  5:168403/148‑1 (MQ=255)
                                                          gcttttttATTGATCATTTACTGCGTTGTGTGCTTCGCGGGATTGCCGGCTCCCACTGGTTCAGCACATGTATGAGGGTTGTGCTGGACCTGTGGTTGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTa                                                                                      <  6:262742/142‑1 (MQ=255)
                                                              aTGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGaa                                                                                   <  2:40691/148‑1 (MQ=255)
                                                                    tGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCAc                                                                             <  4:273588/148‑1 (MQ=255)
                                                                     gATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCAAAGGTTCAGCACAATTGTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGGATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGc                                                                           >  6:37206/1‑149 (MQ=255)
                                                                        cATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGGTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATATGTCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCAAg                                                                        >  2:155728/1‑149 (MQ=255)
                                                                        cATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAg                                                                        >  5:194225/1‑149 (MQ=255)
                                                                          ttGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGcc                                                                                                                   >  1:76212/1‑104 (MQ=255)
                                                                          ttGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGcc                                                                                                                   <  2:76212/104‑1 (MQ=255)
                                                                              ccGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGaaa                                                                  <  8:174313/149‑1 (MQ=255)
                                                                                gcgTCGTCTGCTTCGCGGGCATGCCCGCTCCCACCGGTTGAGCACAACTCTGAGGGGTGTGGTGGCGCTGTGGGAGCGGGCGCGCCCGGGAATGGGGCGGTTAAGGCGCCGCCGCGGGGCCCGGGTAGGGGTCCACGCGAGCCCAAAGc                                                                >  2:190425/1‑149 (MQ=16)
                                                                                 cgtcgtCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCt                                                               >  1:280990/1‑149 (MQ=255)
                                                                                     gtcTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGa                                                             >  5:11537/1‑147 (MQ=255)
                                                                                      tcTGCTTCGAGGGCATGCCCGCTCCCACAGGTTCACCACAAGACTGAGGGTTGAGCTGGAGCTGTGGGAGCGGGCGCGCCCGCGAACAGGACGTATCTGCAGCCCCGGCGGGCCCCGGTTACAAGTCGACGCAAGCAGCAACCTGATAg                                                          >  8:109885/1‑149 (MQ=25)
                                                                                                        ccGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAg                                                          >  1:268831/1‑131 (MQ=255)
                                                                                                              ccACCGGTGCAGCACACTGCTGCGGGTTGGGCTGGACGTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCa                                    <  3:216060/147‑1 (MQ=255)
                                                                                                              ccACAGGTTCAGCACATGGCTGCGGGTTGTGCTGGACCTGTGGTAGGGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCa                                    <  4:144129/147‑1 (MQ=255)
                                                                                                              ccACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAgg                                  >  1:246654/1‑149 (MQ=255)
                                                                                                              ccACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAgg                                  <  3:62293/149‑1 (MQ=255)
                                                                                                                 caGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTc                               >  2:279010/1‑149 (MQ=255)
                                                                                                                  aGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCt                              >  5:194895/1‑149 (MQ=255)
                                                                                                                    gtccAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTgg                            >  8:130713/4‑149 (MQ=37)
                                                                                                                    gtccAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCATGCCCGCGAAGCGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAgg                                  <  2:214379/140‑1 (MQ=25)
                                                                                                                    gtccAGCACAACCCTCAGACTTGTGCTGAACCTGTGGGAGCGGGCATGCCCGCGAAGCGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAgg                                  >  1:214379/4‑143 (MQ=25)
                                                                                                                    gTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATa                                                           <  7:218875/118‑1 (MQ=255)
                                                                                                                    gTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATa                                                           >  8:218875/1‑118 (MQ=255)
                                                                                                                          cacaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTgg                            >  6:120961/1‑143 (MQ=255)
                                                                                                                          cacaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTgg                            <  5:120961/143‑1 (MQ=255)
                                                                                                                          cacaAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGt                      >  6:100427/1‑149 (MQ=255)
                                                                                                                               gTCTGAGGGTTGTGCTGGACGTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGgcgc                                               <  5:166695/119‑1 (MQ=255)
                                                                                                                               gTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGgcgc                                               >  6:166695/1‑119 (MQ=255)
                                                                                                                                      ggTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGCACACCCCag          <  6:194895/149‑1 (MQ=255)
                                                                                                                                      ggTTGGGCTGGACCGGTGGTAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGCACACCCCag          <  6:194225/149‑1 (MQ=255)
                                                                                                                                              tGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGACGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGCACCCCCCAGTAGTCCtt  >  8:144509/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
TCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGCACACCCCAGTAGTCCTT  >  NC_002947/2958672‑2958962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑