| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,010,385 | C→T | 11.7% | intergenic (‑1291/‑377) | PP_2628 ← / → PP_2631 | ABC transporter ATP‑binding protein/cellulose biosynthesis protein BcsF/YhjT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,010,385 | 0 | C | T | 11.7% | 83.6 / 3.0 | 34 | intergenic (‑1291/‑377) | PP_2628/PP_2631 | ABC transporter ATP‑binding protein/cellulose biosynthesis protein BcsF/YhjT |
| Reads supporting (aligned to +/- strand): ref base C (15/15); new base T (2/2); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.23e-01 | |||||||||||
TACGTCTGGCGTCGACACCTGCCAACCGACTTCGACGCCTTGCTGGCACGCCTGCGGCCGCTGGCCATCTGCGGGTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCGCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTGATGGCGACATTGCCTGCGTGGTTGGCGAAGTGCTGTTCCTGTTCCTGTTCGCCT > NC_002947/3010256‑3010532 | ctcGTCTGGCGTCGACACCTGCCAAGCGACTTCGACGCCTTGCTGGCACGCCTGCGGCCGCTGGCCATCTGCGGGTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTcc < 5:249900/146‑1 (MQ=255) cGTCTGGCGTCGACACCTGCCAACCGACTTCGACGCCTTGCTGGCACGCCTGCGGCCGCTGGCCATCTGCGGGTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTccac < 7:166671/148‑1 (MQ=255) gTCTGGCGTCGACACCTGCCAACCGACTTCGACGCCTTGCTGGCACGCCTGCGGCCGCTGGCCATCTGCGGGTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGACGAAATCGTccacca < 8:141680/149‑1 (MQ=255) gTCTGGCGTCGACACCTGCCAACCGACTTCGACGCCTTGCTGGCACGCCTGCGGCCGCTGGCCATCTGCGGGTTGGTAGCGCCACGGGCGTTAGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTcc > 6:249900/1‑145 (MQ=255) cTGGCGTCGACACCTGCCAACCGACTTCGACGCCTTGCTGGCACGCCTGCGGCCGCTGGCCATCTGCGGGTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGt > 5:273638/1‑149 (MQ=255) gCGTCGACACCTGCCAACCGACTTGGACGCATTGCTGGCAGGCGTGCGGCGGCGGGCATTCTGGGGGTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCATAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGc < 1:259827/149‑1 (MQ=255) gCGTCGACACCTGCCAACCGACTTCGACGCCTTGCTGGCACGCCTGCGGCCGCTGGCCATCTGCGGGTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGtt < 7:332843/147‑1 (MQ=255) gCGTCGACACCTGCCAACCGACTTCGACGCCTTGCTGGCACGCCTGCGGCCGCTGGCCATCTGCGGGTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGc < 6:273638/149‑1 (MQ=255) atCGACACCTGCCAACCGACTTCGACGCCTTGCTGGCACGCCTGCGGCCGCTGGCCATCTGCGGGTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTg > 7:67892/2‑149 (MQ=255) cTGCCAACCGACTTCGACGCCTTGCTGGCACGCCTGCGGCCGCTGGCCATCTGCGGGTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAAACGTCCACCAGTTGCTGGTGTTGCg > 6:129102/1‑149 (MQ=255) tGCCAACCGACTTCGACGCCTTGCTGGCACGCCTGCGGCCGCTGGCCATCTGCGGGTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGt > 8:173098/1‑149 (MQ=255) gCACGCCTGCGGCCGCTGGCCATCTGCGGGTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCGCCTGGCCTGACCCCGCTg > 4:10422/1‑147 (MQ=255) gCACGCCTGCGGCCGCTGGCCATCTGCGGGTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGATGCGTCCGGCGCCTGGCCTGACCCCGCTg > 7:160210/1‑147 (MQ=255) ccTGCGGCCGCTGGCCATCTGCGGGTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCGCCTGGCCTGACCCCGCTGCAGGCtt < 7:173098/149‑1 (MQ=255) cATCTGCGGGTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGTGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCgcctggc > 1:299565/1‑115 (MQ=255) cATCTGCGGGTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGTGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCgcctggc < 6:122685/115‑1 (MQ=255) cATCTGCGGGTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGTGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCgcctggc > 5:122685/1‑115 (MQ=255) cATCTGCGGGTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGTGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCgcctggc < 2:299565/115‑1 (MQ=255) gTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCGCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTg > 7:331902/1‑149 (MQ=255) ggCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGCTGCGTCCGGCGCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTGATGGCGACATTGCCt < 8:160210/149‑1 (MQ=255) gCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCTCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTGATGGCGACAtt < 8:48653/144‑1 (MQ=255) gCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCTCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTGATGGCGACAtt > 7:48653/1‑144 (MQ=255) cGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCGCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTGATGGCGACATTGCCTGc > 6:280237/1‑149 (MQ=255) ttCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCGCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTGa < 4:51381/131‑1 (MQ=255) ttCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCGCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTGa > 3:51381/1‑131 (MQ=255) cGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAAACGTCCACCAGTTGCTGGTGTTGCGTCCGGCGCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTGATGGCGACATTGCCTGCGTg < 5:129102/148‑1 (MQ=255) cGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCGCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTGATGGCGACATTGCCTGCGTGGTTGGCg > 1:86279/1‑146 (MQ=255) cGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCGCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTGATGGCGACATTGCCTGCGTGGTTGGCg < 2:86279/146‑1 (MQ=255) gTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCGCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTGATGGCGACATTGCCTGCGTGGTTGGCGAAGt > 1:332232/1‑149 (MQ=255) tGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCGCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTGATGGCGACATTGCCTGCGTGGTTGGCGAAGTg > 5:328614/1‑149 (MQ=255) gCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCGCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTGATGGCGACATTGCCTGCGTGGTTGGCGAAGTGc > 5:185121/1‑149 (MQ=255) aaaGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCGCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTGATGGCGACATTGCCTGCGTGGTTGGCGAAGTGctg < 5:280237/149‑1 (MQ=255) cGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCGCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTGATGGCGACATTGCCTGCGTGGTTGGCGAAGTGctgttcctgttcctgt < 6:185121/149‑1 (MQ=255) tGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCGCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTGATGGCGACATTGCCTGCGTGGTTGGCGAAGTGCTGTTCCTGTTCCTGTTCGcc < 4:70955/149‑1 (MQ=255) cgcAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCGCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTGATGGCGACATTGCCTGCGTGGTTGGCGAAGTGCTGTTCCTGTTCCTGTTCGCCt < 2:332232/148‑1 (MQ=255) cgcAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCGCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTGATGGCGACATTGCCTGCGTGGTTGGCGAAGTGCTGTTCCTGTTCCTGTTCGCCt < 8:67892/148‑1 (MQ=255) | TACGTCTGGCGTCGACACCTGCCAACCGACTTCGACGCCTTGCTGGCACGCCTGCGGCCGCTGGCCATCTGCGGGTTGGTAGCGCCACGGGCGTTCGCCGATGCCGTGCAAAGCATGTGGCACGGGGTGCGCAACGGCGAAATCGTCCACCAGTTGCTGGTGTTGCGTCCGGCGCCTGGCCTGACCCCGCTGCAGGCTTGCTCGCGTACGGTATTCCGCCGTGATGGCGACATTGCCTGCGTGGTTGGCGAAGTGCTGTTCCTGTTCCTGTTCGCCT > NC_002947/3010256‑3010532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |