| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,046,441 | G→C | 8.2% | R43P (CGT→CCT) | pstB‑I → | phosphate ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,046,441 | 0 | G | C | 8.2% | 149.6 / 4.1 | 49 | R43P (CGT→CCT) | pstB‑I | phosphate ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (26/19); new base C (2/2); total (28/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01 | |||||||||||
TCTTCGGCAAGCCCAAGTATTGAGAAAGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCTTCGAAGGCCATGTGCACTTTCTTGG > NC_002947/3046295‑3046588 | tCTTCGGCAAGCCCAAGTATTGAGAAAGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCcgtgcgt > 6:267080/1‑148 (MQ=255)tCTTCAGAAAGACGAAGTATTGAGAAACGGAGCATACAACTTGAACGTATCCAATGCGCAAAGAGCCGCTACCATGGTCAGCGAAGCCCCCAACGGCAACGACTGCAAGCTCGACAAGCTTTTATACGGTACCATCATAGCagcgcgt > 8:273784/1‑148 (MQ=255) cAAGTATTGAGAAAGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGcc < 2:99894/149‑1 (MQ=255) gTATTGAGAAAGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGAt < 6:269398/149‑1 (MQ=255) agaAAGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGa > 4:8936/1‑148 (MQ=255) agaAAGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGa > 4:249189/1‑148 (MQ=255) agaAAGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGa > 8:275011/1‑148 (MQ=255) agaAAGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGa < 3:248979/148‑1 (MQ=255) aaaGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGaaa > 5:354206/1‑148 (MQ=255) gggAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAAc > 6:115948/1‑148 (MQ=255) ggAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACga < 6:47887/149‑1 (MQ=255) gAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCcgtgcgt < 2:59506/119‑1 (MQ=255) gAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAAc > 5:28544/1‑146 (MQ=255) tCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCAc > 3:5925/1‑149 (MQ=255) gAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCa > 4:78223/1‑149 (MQ=255) gTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCgg < 4:5925/149‑1 (MQ=255) gcAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCt < 1:219600/149‑1 (MQ=255) cGCTCCCATGGTCAGCGAAGCCCCCATCCTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAaga < 7:275011/149‑1 (MQ=255) cTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCCGCGGCAAGAGt < 6:27520/149‑1 (MQ=255) aTGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGt > 4:174664/1‑149 (MQ=255) ggTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGCCAGCCATGTGCCGATCAGGAAAAACGAGATCGCCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGtgc > 4:237141/1‑149 (MQ=255) gTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGtgct > 3:63240/1‑149 (MQ=255) tCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGtgctg < 3:249189/149‑1 (MQ=255) aGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGtgctgc < 3:129686/148‑1 (MQ=255) aGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGtgctgc < 4:7321/148‑1 (MQ=255) cccATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGaa < 5:267080/149‑1 (MQ=255) ccATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCGGCGTAGCTTGAAc > 2:124167/1‑149 (MQ=255) aCTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTatgatggac > 8:301495/1‑144 (MQ=255) cAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTatg > 4:278410/1‑139 (MQ=255) cAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTatg < 3:278410/139‑1 (MQ=255) cAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTgg > 1:321677/1‑149 (MQ=255) gCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTg > 6:106066/1‑148 (MQ=255) gACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAggg > 7:178647/1‑149 (MQ=255) aaGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTACATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGAATGAATGACCTGGTGAAGGGCtt > 3:66210/1‑149 (MQ=255) aGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTc < 6:28544/149‑1 (MQ=255) aTTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCg > 4:15384/1‑149 (MQ=255) ttttCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGc > 5:305511/1‑149 (MQ=255) tttCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCt > 6:124826/1‑149 (MQ=255) tACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCTTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCTTCg > 5:228507/1‑148 (MQ=255) cGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCTTCGaa < 3:78223/148‑1 (MQ=255) aCTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCTTCGAAGGCCAt < 3:15384/149‑1 (MQ=255) cTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCTTCGAAGGCCAtg > 4:114799/1‑149 (MQ=255) cTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCTTCGAAGGCCAtg < 6:354206/149‑1 (MQ=255) cTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCTTCGAAGGCCAtg < 2:234790/149‑1 (MQ=255) tCATGGCCGTGCGTGACCGCCATGTGCCGATCAGGAAACACGAGATCACCGGTTTCATCGGCCCATCGGGCTGCGCCAAGAGAACCGTGCTTCCGAGCTGGAACCGAATGAATGACGGGGTGACGGGCTTCCGCTGCGAGGGcccggag > 8:251665/1‑144 (MQ=255) tCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCTTCGACGGCCAt < 6:152042/146‑1 (MQ=255) tCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCTTCGAAGGCCAt > 5:152042/1‑146 (MQ=255) ccGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCTTCGAAGGCCATGTGCACttt > 3:238638/1‑149 (MQ=255) ccGTGCCTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGa > 6:42726/1‑96 (MQ=255) ccGTGCCTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGa < 5:48065/96‑1 (MQ=255) ccGTGCCTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGa > 6:48065/1‑96 (MQ=255) ccGTGCCTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGa < 5:42726/96‑1 (MQ=255) cgtgcgtgACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCTTCGAAGGCCAt < 7:174248/139‑1 (MQ=255) cgtgcgtgACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCTTCGAAGGCCAt > 8:174248/1‑139 (MQ=255) gtgcgtgACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGc < 2:307070/90‑1 (MQ=255) gtgcgtgACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGc > 1:307070/1‑90 (MQ=255) gcgtgACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTACGCTTCGAAGGCCATGTGCACTTTCttt > 5:51632/1‑148 (MQ=255) cgtgACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCTTCGAAGGCCATGTGCACTTTCTTgg < 8:178647/149‑1 (MQ=255) cgtgACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCTTCGAAGGCCATGTGCACTTTCTTgg < 4:238638/149‑1 (MQ=255) cgtgACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCTTCGAAGGCCATGTGCACTTTCTTg < 5:106066/148‑1 (MQ=255) | TCTTCGGCAAGCCCAAGTATTGAGAAAGGGAGCATCCAACTTGAACGTATCCACTGCGCAAAGAGCCGCTCCCATGGTCAGCGAAGCCCCCATCGTCATGGACTGCAAGCTCGACAAGATTTTCTACGGTAACTTCATGGCCGTGCGTGACAGCCATGTGCCGATCAGGAAAAACGAGATCACCGGTTTCATCGGCCCTTCGGGCTGCGGCAAGAGTACCGTGCTGCGTAGCTTGAACCGTATGAATGACCTGGTGAAGGGCTTCCGCTTCGAAGGCCATGTGCACTTTCTTGG > NC_002947/3046295‑3046588 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |