Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,051,838 T→A 10.3% Y180N (TAC→AAC) ‡ PP_2664 → two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,051,8380TA10.3% 152.4 / 8.5 58Y180N (TAC→AAC) ‡PP_2664two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base T (24/28);  new base A (3/3);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

GCCGCATGGCGTGATGCTGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGAGCAACGGGTGCGCGAGCG  >  NC_002947/3051690‑3051986
                                                                                                                                                     |                                                                                                                                                    
gCCGCATGGCGTGATGCTGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCt                                                                                                                                                      <  5:144147/149‑1 (MQ=255)
        gCGTGATGCTGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCAGCAtgta                                                                                                                                               <  4:225008/148‑3 (MQ=255)
         cGTGATGCTGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCATCACTACCAGCGCCTGTAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGATTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACAtgctgc                                                                                                                                             <  3:199499/149‑1 (MQ=255)
         cGTGATGCTGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACAtgctgc                                                                                                                                             <  1:360777/149‑1 (MQ=255)
           tGATGCTGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGt                                                                                                                                           <  2:35015/149‑1 (MQ=255)
            gATGCTGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGtt                                                                                                                                          >  6:74665/1‑149 (MQ=255)
               gCTGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTcc                                                                                                                                        >  8:84317/1‑148 (MQ=255)
                cTGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCt                                                                                                                                       <  1:231198/148‑1 (MQ=255)
                 tGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTAc                                                                                                                                     >  5:318483/1‑149 (MQ=255)
                   ggCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCAGCATGTAGCGTTCCtgg                                                                                                                                     <  8:135176/147‑3 (MQ=255)
                           ccTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGGTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAAcc                                                                                                                           >  4:351334/1‑149 (MQ=255)
                                gcgAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTg                                                                                                                      <  8:293104/149‑1 (MQ=255)
                                 cgAGGCCCACAGCGATCAGCACTACCAGCGGCTGGAAGGCTATGTTGCGCGGGAACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTgg                                                                                                                     <  7:149854/149‑1 (MQ=255)
                                 cgAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCAGCATGTAGCGTTCCtg                                                                                                                                      >  2:265138/1‑131 (MQ=255)
                                 cgAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTCCGCCGAGACCAATATCAACGGCCAGGAACGCAGCATGTAGCGTTCCtg                                                                                                                                      <  1:265138/132‑2 (MQ=255)
                                   aGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTTCCGAACCGCCTGGcc                                                                                                                   <  7:84317/149‑1 (MQ=255)
                                       ccACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTg                                                                                                               >  2:39258/1‑149 (MQ=255)
                                            gCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGaa                                                                                                          <  3:6731/149‑1 (MQ=255)
                                                  agcaCTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAgtggtg                                                                                                    <  6:148506/149‑1 (MQ=255)
                                                  agcaCTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAgtggtg                                                                                                    <  1:35262/149‑1 (MQ=255)
                                                   gcaCTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGGGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAgtggttg                                                                                                   <  1:88663/149‑3 (MQ=255)
                                                     aCTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGt                                                                                                 <  4:19693/149‑1 (MQ=255)
                                                          cAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCt                                                                                                                                       <  4:87248/106‑1 (MQ=255)
                                                          cAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCt                                                                                                                                       >  3:87248/1‑106 (MQ=255)
                                                          cAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCtt                                                                                            >  4:191850/1‑149 (MQ=255)
                                                            gcgcCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCg                                                                                          >  6:184618/1‑149 (MQ=255)
                                                              gcCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTg                                                                                        <  2:247326/149‑1 (MQ=255)
                                                              gcCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTg                                                                                        <  4:84368/149‑1 (MQ=255)
                                                              gcCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTg                                                                                        >  1:247326/1‑149 (MQ=255)
                                                              gcCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTg                                                                                        <  5:74665/149‑1 (MQ=255)
                                                               ccTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTc                                                                                           >  1:255713/1‑145 (MQ=255)
                                                               ccTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTc                                                                                           >  1:250626/1‑145 (MQ=255)
                                                               ccTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTc                                                                                           <  2:255713/145‑1 (MQ=255)
                                                               ccTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTc                                                                                           <  2:250626/145‑1 (MQ=255)
                                                                 tGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTg                                                                                        <  1:329034/146‑1 (MQ=255)
                                                                 tGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTg                                                                                        >  2:329034/1‑146 (MQ=255)
                                                                       gCTATGTGGCGCGGGCACTGGCGGGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCcg                                                                                >  1:198521/1‑149 (MQ=255)
                                                                           tgtgGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGa                                                                             <  5:184618/147‑1 (MQ=255)
                                                                             tgGCGCGGGCACTGGC‑GGGGGAGAGAGTGACGTTCGAGTTCGCCGAGAACAATATCAACGGCCAGGAACGCTACATGCTGGGTTCATACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACAt                                                                          >  7:225797/1‑148 (MQ=255)
                                                                                                  gagaGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGtggtgg                                                                                                   >  6:227963/1‑103 (MQ=255)
                                                                                                     agCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGcaca                                                  <  3:191850/149‑1 (MQ=255)
                                                                                                        gTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGCCATCACCGAGCGCCGCAACACCGCACAGGc                                               >  8:3746/1‑149 (MQ=255)
                                                                                                         tGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCg                                              >  4:148580/1‑149 (MQ=255)
                                                                                                          gACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGt                                             >  3:53736/1‑149 (MQ=255)
                                                                                                          gACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGt                                             >  7:98229/1‑149 (MQ=255)
                                                                                                                cGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCAc                                        <  3:351334/148‑1 (MQ=255)
                                                                                                                   gTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAgg                                    <  6:318483/149‑1 (MQ=255)
                                                                                                                   gTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAgg                                    >  8:246176/1‑149 (MQ=255)
                                                                                                                         cGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATc                              <  7:246176/149‑1 (MQ=255)
                                                                                                                              ccAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCAc                         >  1:190545/1‑149 (MQ=255)
                                                                                                                               cAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCAcc                        >  7:254590/1‑149 (MQ=255)
                                                                                                                                 atatCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTg                      <  2:198521/149‑1 (MQ=255)
                                                                                                                                  tatCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTgg                     <  2:190545/149‑1 (MQ=255)
                                                                                                                                    tCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGa                    <  1:39258/148‑1 (MQ=255)
                                                                                                                                         ggCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGAGCAACg              >  1:143025/1‑149 (MQ=255)
                                                                                                                                         acgtaGGAACGCAGCATGTAGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGAGCAACg              >  7:205941/5‑149 (MQ=255)
                                                                                                                                              ggAACGCAGCATGTAGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGAGCAACGGGTgc         >  3:32142/1‑149 (MQ=255)
                                                                                                                                                  cGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGAGCAACGGGTGCGCGa     <  8:225797/149‑1 (MQ=255)
                                                                                                                                                    cTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGAGCAACGGGTGCGCGAgc   >  2:205206/1‑149 (MQ=255)
                                                                                                                                                     tACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGAGCAACGGGTGCGCGAgcg  >  7:314295/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCCGCATGGCGTGATGCTGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGAGCAACGGGTGCGCGAGCG  >  NC_002947/3051690‑3051986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑