Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,051,844	C→T	8.9%	L182L (CTG→TTG) ‡	PP_2664 →	two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,051,844	0	C	T	8.9%	154.7 / 4.5	56	L182L (CTG→TTG) ‡	PP_2664	two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base C (25/26);  new base T (3/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

CGTGATGCTGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGAGCAACGGGTGCGCGAGCGCACTGC  >  NC_002947/3051699‑3051992                                                                                                                                                   |                                                                                                                                                     cGTGATGCTGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCATCACTACCAGCGCCTGTAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGATTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACAtgctgc                                                                                                                                                   <  3:199499/149‑1 (MQ=255) cGTGATGCTGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACAtgctgc                                                                                                                                                   <  1:360777/149‑1 (MQ=255)   tGATGCTGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGt                                                                                                                                                 <  2:35015/149‑1 (MQ=255)    gATGCTGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGtt                                                                                                                                                >  6:74665/1‑149 (MQ=255)       gCTGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTcc                                                                                                                                              >  8:84317/1‑148 (MQ=255)        cTGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCt                                                                                                                                             <  1:231198/148‑1 (MQ=255)         tGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTAc                                                                                                                                           >  5:318483/1‑149 (MQ=255)           ggCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCAGCATGTAGCGTTCCtgg                                                                                                                                           <  8:135176/147‑3 (MQ=255)                   ccTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGGTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAAcc                                                                                                                                 >  4:351334/1‑149 (MQ=255)                        gcgAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTg                                                                                                                            <  8:293104/149‑1 (MQ=255)                         cgAGGCCCACAGCGATCAGCACTACCAGCGGCTGGAAGGCTATGTTGCGCGGGAACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTgg                                                                                                                           <  7:149854/149‑1 (MQ=255)                         cgAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCAGCATGTAGCGTTCCtg                                                                                                                                            >  2:265138/1‑131 (MQ=255)                         cgAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTCCGCCGAGACCAATATCAACGGCCAGGAACGCAGCATGTAGCGTTCCtg                                                                                                                                            <  1:265138/132‑2 (MQ=255)                           aGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTTCCGAACCGCCTGGcc                                                                                                                         <  7:84317/149‑1 (MQ=255)                               ccACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTg                                                                                                                     >  2:39258/1‑149 (MQ=255)                                    gCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGaa                                                                                                                <  3:6731/149‑1 (MQ=255)                                          agcaCTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAgtggtg                                                                                                          <  6:148506/149‑1 (MQ=255)                                          agcaCTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAgtggtg                                                                                                          <  1:35262/149‑1 (MQ=255)                                           gcaCTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGGGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAgtggttg                                                                                                         <  1:88663/149‑3 (MQ=255)                                             aCTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGt                                                                                                       <  4:19693/149‑1 (MQ=255)                                                  cAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCt                                                                                                                                             <  4:87248/106‑1 (MQ=255)                                                  cAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCt                                                                                                                                             >  3:87248/1‑106 (MQ=255)                                                  cAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCtt                                                                                                  >  4:191850/1‑149 (MQ=255)                                                    gcgcCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCg                                                                                                >  6:184618/1‑149 (MQ=255)                                                      gcCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTg                                                                                              >  1:247326/1‑149 (MQ=255)                                                      gcCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTg                                                                                              <  2:247326/149‑1 (MQ=255)                                                      gcCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTg                                                                                              <  5:74665/149‑1 (MQ=255)                                                      gcCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTg                                                                                              <  4:84368/149‑1 (MQ=255)                                                       ccTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTc                                                                                                 <  2:255713/145‑1 (MQ=255)                                                       ccTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTc                                                                                                 <  2:250626/145‑1 (MQ=255)                                                       ccTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTc                                                                                                 >  1:250626/1‑145 (MQ=255)                                                       ccTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTc                                                                                                 >  1:255713/1‑145 (MQ=255)                                                         tGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTg                                                                                              <  1:329034/146‑1 (MQ=255)                                                         tGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTg                                                                                              >  2:329034/1‑146 (MQ=255)                                                               gCTATGTGGCGCGGGCACTGGCGGGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCcg                                                                                      >  1:198521/1‑149 (MQ=255)                                                                   tgtgGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGa                                                                                   <  5:184618/147‑1 (MQ=255)                                                                     tgGCGCGGGCACTGGC‑GGGGGAGAGAGTGACGTTCGAGTTCGCCGAGAACAATATCAACGGCCAGGAACGCTACATGCTGGGTTCATACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACAt                                                                                >  7:225797/1‑148 (MQ=255)                                                                                          gagaGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGtggtgg                                                                                                         >  6:227963/1‑103 (MQ=255)                                                                                             agCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGcaca                                                        <  3:191850/149‑1 (MQ=255)                                                                                                gTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGCCATCACCGAGCGCCGCAACACCGCACAGGc                                                     >  8:3746/1‑149 (MQ=255)                                                                                                 tGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCg                                                    >  4:148580/1‑149 (MQ=255)                                                                                                  gACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGt                                                   >  7:98229/1‑149 (MQ=255)                                                                                                  gACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGt                                                   >  3:53736/1‑149 (MQ=255)                                                                                                        cGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCAc                                              <  3:351334/148‑1 (MQ=255)                                                                                                           gTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAgg                                          >  8:246176/1‑149 (MQ=255)                                                                                                           gTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAgg                                          <  6:318483/149‑1 (MQ=255)                                                                                                                 cGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATc                                    <  7:246176/149‑1 (MQ=255)                                                                                                                      ccAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCAc                               >  1:190545/1‑149 (MQ=255)                                                                                                                       cAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCAcc                              >  7:254590/1‑149 (MQ=255)                                                                                                                         atatCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTg                            <  2:198521/149‑1 (MQ=255)                                                                                                                          tatCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTgg                           <  2:190545/149‑1 (MQ=255)                                                                                                                            tCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGa                          <  1:39258/148‑1 (MQ=255)                                                                                                                                 ggCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGAGCAACg                    >  1:143025/1‑149 (MQ=255)                                                                                                                                 acgtaGGAACGCAGCATGTAGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGAGCAACg                    >  7:205941/5‑149 (MQ=255)                                                                                                                                      ggAACGCAGCATGTAGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGAGCAACGGGTgc               >  3:32142/1‑149 (MQ=255)                                                                                                                                          cGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGAGCAACGGGTGCGCGa           <  8:225797/149‑1 (MQ=255)                                                                                                                                            cTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGAGCAACGGGTGCGCGAgc         >  2:205206/1‑149 (MQ=255)                                                                                                                                             tACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGAGCAACGGGTGCGCGAgcg        >  7:314295/1‑149 (MQ=255)                                                                                                                                                  gctgcGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGAGCAACgg                   >  6:292307/1‑133 (MQ=255)                                                                                                                                                  gctgcGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGAGCAACgg                   <  5:292307/133‑1 (MQ=255)                                                                                                                                                   ctgcGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGAGCAACGGGTGCGCGAGCGCACTGc  <  1:167681/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     CGTGATGCTGGGCCAGAGCCTGCGCGAGGCCCACAGCGAGCAGCACTACCAGCGCCTGGAAGGCTATGTGGCGCGGGCACTGGC‑GGGGGAGAGCGTGACGTTCGAGTTCGCCGAGACCAATATCAACGGCCAGGAACGCTACATGCTGCGTTCCTACGTACCGAACCGCCTGGCCAGTGGCGAAGTGGTGGGTATCTTCGTGCTGATCCGCGACATCACCGAGCGCCGCAACACCGCACAGGCGTTGCACCAGGCGTATCAGCACCTGGAGCAACGGGTGCGCGAGCGCACTGC  >  NC_002947/3051699‑3051992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑