Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,068,537	G→A	7.9%	E526K (GAA→AAA) ‡	qedH‑II →	quinoprotein ethanol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,068,537	0	G	A	7.9%	179.6 / 4.4	64	E526K (GAA→AAA) ‡	qedH‑II	quinoprotein ethanol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (29/29);  new base A (3/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.57e-01

CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGG  >  NC_002947/3068389‑3068685                                                                                                                                                     |                                                                                                                                                      cccGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTggg                                                                                                                                                       <  4:43598/149‑1 (MQ=255)  ccGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTggg                                                                                                                                                       >  2:27461/1‑148 (MQ=255)  ccGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGa                                                                                                                                                      <  3:60127/149‑1 (MQ=255)     gTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTTGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAAtt                                                                                                                                                   <  5:294929/149‑1 (MQ=255)          cGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGa                                                                                                                                             >  5:106690/1‑149 (MQ=255)           ggCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑c                                                                                                                                                 <  4:255025/144‑1 (MQ=255)           ggCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGat                                                                                                                                            >  3:255025/1‑148 (MQ=255)                   aGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCACCGCCAAGACCGGCGACAAGGTCTGGAATTC‑CCAGAccttgtcgc                                                                                                                                    <  6:13952/149‑8 (MQ=38)                       tgGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGAGTGCTGACCACCAAGGTCAAGCAGGTGTTCAAGGGAACGAAAGAGGGCTTCCTGCAGGCATTAAACGCCAAGACCGTCGACAACGTCTGGGAAGT‑GCAGACAGGCTCGGGCGt                                                                                                                                >  8:89047/1‑149 (MQ=255)                         gcgcCACAAGAACTATGCGCCGCTGTGGGGCGGAGTTATGACCAAAAAGGGCAACGTGAGGTACACGGACCCTCCAGAGGGCTTACAGGATGCATTCAACTCCAATACAGGCTACAAGCACTGGCAATA‑AAAAAAAGGCTCGGGCGTgc                                                                                                                              >  6:274610/1‑149 (MQ=255)                          cgcCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGGCTTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTgct                                                                                                                             <  1:279769/149‑1 (MQ=255)                                   aaCTATGCGACGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACTCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCgg                                                                                                                                    >  2:284216/1‑133 (MQ=255)                                        tGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGt                                                                                                                 <  8:85155/147‑1 (MQ=255)                                        tGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGt                                                                                                                 >  7:85155/1‑147 (MQ=255)                                             cgcTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTgg                                                                                                          <  1:35423/149‑1 (MQ=255)                                                      gCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGAc                                                                                                 <  3:309308/149‑1 (MQ=255)                                                                gACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCaa                                                                                        >  5:269806/1‑148 (MQ=255)                                                                 accaccAAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAAt                                                                                       <  6:106690/148‑1 (MQ=255)                                                                      caAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGttt                                                                                 >  1:58598/1‑149 (MQ=255)                                                                      caAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGttt                                                                                 >  8:142182/1‑149 (MQ=255)                                                                        aGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCg                                                                               >  2:186457/1‑149 (MQ=255)                                                                           gCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATg                                                                                                    <  6:2911/125‑1 (MQ=255)                                                                           gCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATg                                                                                                    >  5:2911/1‑125 (MQ=255)                                                                                   gtgtTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCgg                                                                    >  2:332587/1‑149 (MQ=255)                                                                                    tgtTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGc                                                                   >  1:30814/1‑149 (MQ=255)                                                                                      ttCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGGCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTg                                                                 >  4:244589/1‑149 (MQ=255)                                                                                        cACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTggg                                                               >  5:6403/1‑149 (MQ=255)                                                                                                 gCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCgg                                                      <  7:142182/149‑1 (MQ=255)                                                                                                  ccAGAGGGCTTCCTGCAGGCATTCCCCGCCAAGCCCGGCGACAAGGTCTGGGAAGT‑CCAGACCCGCTCGGGCGTGCGCGGCTCGCCCGGCACCTGGCAAAGGGCCGGCGCGCAATACGGTGCGGTAGGCTCCGGCTGGGGCGGCGCGGt                                                     >  5:92773/1‑149 (MQ=255)                                                                                                   cAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTg                                                    >  4:333682/1‑149 (MQ=255)                                                                                                              cTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTggg                                          <  6:269806/148‑1 (MQ=255)                                                                                                               tGCAGGCATTCAACGCCAAGACCGGAGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGggc                                        >  4:3177/1‑149 (MQ=255)                                                                                                                gCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGggcg                                       >  4:122737/1‑149 (MQ=255)                                                                                                                 cAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGggcgg                                      >  4:334656/1‑149 (MQ=255)                                                                                                                  aGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAACTGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGggcggc                                     <  5:215438/149‑1 (MQ=255)                                                                                                                  aGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGggcggc                                     >  1:320701/1‑149 (MQ=255)                                                                                                                    gCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTggg                                          >  1:123162/1‑142 (MQ=255)                                                                                                                    gCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTggg                                          <  2:123162/142‑1 (MQ=255)                                                                                                                     cATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGGTGTGGGGCGGCGaa                                  <  3:333682/149‑1 (MQ=255)                                                                                                                      aTTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAg                                 >  7:232211/1‑149 (MQ=255)                                                                                                                       ttCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGggcggc                                     >  8:340134/1‑144 (MQ=255)                                                                                                                       ttCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGggcggc                                     <  7:340134/144‑1 (MQ=255)                                                                                                                       ttCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGt                                >  5:179976/1‑149 (MQ=255)                                                                                                                       ttCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGt                                <  7:79051/149‑1 (MQ=255)                                                                                                                         cAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTgg                              >  4:249943/1‑149 (MQ=255)                                                                                                                         cAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTg                               <  2:58598/148‑1 (MQ=255)                                                                                                                         cAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTg                               <  2:30814/148‑1 (MQ=255)                                                                                                                          aaCGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGc                             <  2:320701/149‑1 (MQ=255)                                                                                                                           aCGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCg                                                                               <  1:169597/98‑1 (MQ=255)                                                                                                                           aCGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCg                                                                               >  2:169597/1‑98 (MQ=255)                                                                                                                               cAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAAc                        >  7:8035/1‑149 (MQ=255)                                                                                                                                 aGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACgg                      <  1:186457/149‑1 (MQ=255)                                                                                                                                      ggCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGCCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCaa                 <  3:244589/149‑1 (MQ=255)                                                                                                                                       gCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAg                <  6:6403/149‑1 (MQ=255)                                                                                                                                       gCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAg                <  3:334656/149‑1 (MQ=255)                                                                                                                                       gCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAg                <  1:332587/149‑1 (MQ=255)                                                                                                                                         gagccggTCTGGAATTC‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAg                >  4:128540/6‑147 (MQ=255)                                                                                                                                         gACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGa              >  3:125369/1‑149 (MQ=255)                                                                                                                                         gACAAGGTCTGGGAATT‑CCAGACCGGATCGGGCTTGCTCGGATCTCCCGTCTCCTGGGATATTGACTGCGAGCAATACGTTTCGGTAGTCTGCGGCTGGTGCTGCGCGTTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGa              <  5:274610/149‑1 (MQ=255)                                                                                                                                           cAAGGTCTGGGACTT‑CCAGTCCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGAAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGGCAAGTACt            <  6:92773/149‑1 (MQ=255)                                                                                                                                             cggTCTGGAATTC‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGt                                >  3:296296/2‑127 (MQ=255)                                                                                                                                             cggTCTGGAATTC‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGt                                <  4:296296/126‑1 (MQ=255)                                                                                                                                               gTCTGGAATTC‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCaa        >  7:182853/1‑149 (MQ=255)                                                                                                                                                 cTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGTGCGTCGCTGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAAcc      <  7:89047/149‑1 (MQ=255)                                                                                                                                                  tGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTc          >  7:6442/1‑144 (MQ=255)                                                                                                                                                  tGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTc          <  8:6442/144‑1 (MQ=255)                                                                                                                                                    ggAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAgg   >  8:25558/1‑149 (MQ=255)                                                                                                                                                     gAATTCCCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTg                               >  7:263197/1‑121 (MQ=255)                                                                                                                                                     gAATTCCCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTg                               <  8:263197/121‑1 (MQ=255)                                                                                                                                                     gAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAggg  >  3:122230/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                      CCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGCTTCCTGCAGGCATTCAACGCCAAGACCGGCGACAAGGTCTGGGAATT‑CCAGACCGGCTCGGGCGTGCTCGGCTCGCCCGTCACCTGGGAAATGGACGGCGAGCAATACGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGG  >  NC_002947/3068389‑3068685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑