Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,121,364 T→A 7.8% Q27L (CAG→CTG)  PP_2737 ← short‑chain dehydrogenase/reductase family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,121,3640TA 7.8% 147.8 / 3.2 51Q27L (CAG→CTG) PP_2737short‑chain dehydrogenase/reductase family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (21/26);  new base A (2/2);  total (23/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.69e-01

CAGGCCTCCCCAGGCCTGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATAAAGCAGGGC  >  NC_002947/3121216‑3121508
                                                                                                                                                    |                                                                                                                                                
cAGGCCTCCCCAGGCCTGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCt                                                                                                                                                  >  2:317749/1‑149 (MQ=255)
     cTCCCCAGGCCTGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGTGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCtggtgg                                                                                                                                             >  1:189391/1‑149 (MQ=255)
      tCCCCAGGCCTGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCCAGGCGACCTGGTGGc                                                                                                                                            >  4:254028/1‑149 (MQ=255)
       ccccAGGCCTGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCCACCAGGTCGcc                                                                                                                                           >  6:153949/1‑149 (MQ=255)
        cccAGGCCTGCTCGATATGCGCAGCGATGCCGGCTACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCt                                                                                                                                          <  7:321789/149‑1 (MQ=255)
        cccAGGCCTGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGc                                                                                                                                            <  7:180720/147‑1 (MQ=255)
          cAGGCCTGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCCACCAGGTCGCCTTg                                                                                                                                        <  5:153949/149‑1 (MQ=255)
              ccTGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTc                                                                                                                                     >  1:201787/1‑148 (MQ=255)
                tGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTcc                                                                                                                                    <  8:42368/147‑1 (MQ=255)
                tGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTcc                                                                                                                                    >  7:42368/1‑147 (MQ=255)
                  cTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCagga                                                                                                                                <  1:232309/149‑1 (MQ=255)
                        atGCGCATCGATGCCGGCGACCTTCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCGTGCCCGGGAAACCGTTCTGACAGCGGGGCATGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACt                                                                                                                           <  5:68478/148‑1 (MQ=255)
                          gcgcAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCtt                                                                                                                                         >  8:14956/1‑132 (MQ=255)
                          gcgcAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCtt                                                                                                                                         <  7:14956/132‑1 (MQ=255)
                               gCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCACGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAg                                                                                                                   >  3:224800/1‑149 (MQ=255)
                                  aTGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGAc                                                                                                                            <  6:244329/137‑1 (MQ=255)
                                  aTGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGAc                                                                                                                            >  5:244329/1‑137 (MQ=255)
                                      cGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCa                                                                                                            >  3:282023/1‑149 (MQ=255)
                                       ggCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCAc                                                                                                           <  6:291803/149‑1 (MQ=255)
                                           aCCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCTGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGa                                                                                                        >  8:162283/1‑148 (MQ=255)
                                                       ttGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGgc                                                                                           >  8:235059/1‑149 (MQ=255)
                                                          tCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGcc                                                                                        >  1:8504/1‑149 (MQ=255)
                                                           cAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCg                                                                                       >  5:78446/1‑149 (MQ=255)
                                                           cAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCg                                                                                       >  1:118811/1‑149 (MQ=255)
                                                               gtCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCa                                                                                   <  2:132091/148‑1 (MQ=255)
                                                               aTCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCagga                                                                                                                                >  2:286473/1‑104 (MQ=255)
                                                                  gCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGc                                                                                 <  2:8504/148‑1 (MQ=255)
                                                                     aaTGGCCAGCAATGCCTGCCCGGGAAACCGTTCAGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCAGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTTCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCa                                                                                   >  7:159462/1‑143 (MQ=255)
                                                                     aaTGGCCAGCAATGACTGCCCGGGAATCCTTTCTGCCAGCGGGGCAAGGCGTTCTGCGTTGCGACCGCCAAAGGCGACCTGGTGGCCTTTCTCCAGTAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCTGTCa                                                                                   <  8:159462/143‑1 (MQ=255)
                                                                          ccAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCgg                                                                        >  2:190724/1‑149 (MQ=255)
                                                                           cAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGc                                                                       >  3:307707/1‑149 (MQ=255)
                                                                             gCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAgc                                                                                                                  >  5:72113/1‑104 (MQ=255)
                                                                             gCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAgc                                                                                                                  <  6:72113/104‑1 (MQ=255)
                                                                                    ctgccCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAg                                                              >  7:324489/1‑149 (MQ=255)
                                                                                       cccGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCTGCTCATGCCAgcct                                                           <  2:189391/149‑1 (MQ=255)
                                                                                       cccGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAgcc                                                            <  1:317749/148‑1 (MQ=255)
                                                                                         cGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAgcctgc                                                         >  5:280468/1‑149 (MQ=255)
                                                                                            gAAACCGTTCTGCCAGCGGGGCAATCCGGTCGGCGTTGAGCCCGACCAAGGCCACCAGGTCGCCTTgg                                                                                                                                       >  2:63058/1‑67 (MQ=21)
                                                                                            gAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCCACCAGGTCGCCTTgg                                                                                                                                       <  1:63058/68‑2 (MQ=38)
                                                                                              aaCCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGc                                                                          <  2:354267/127‑1 (MQ=255)
                                                                                               aCCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGa                                                   <  7:235059/149‑1 (MQ=255)
                                                                                                  gTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGc                                                 >  7:145127/1‑148 (MQ=255)
                                                                                                           gCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTg                                       <  1:190724/149‑1 (MQ=255)
                                                                                                             ggggCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCt                                     <  6:69739/149‑1 (MQ=255)
                                                                                                               ggCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCtgt                                   <  8:145127/149‑1 (MQ=255)
                                                                                                                tcAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCtgtg                                  <  4:282023/148‑1 (MQ=255)
                                                                                                                     gCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCAt                             <  6:280468/149‑1 (MQ=255)
                                                                                                                        gTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGGCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCATGAc                          <  4:224800/149‑1 (MQ=255)
                                                                                                                         tCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCATGACg                         <  6:78446/149‑1 (MQ=255)
                                                                                                                           ggCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCATGACggg                       <  2:118811/149‑1 (MQ=255)
                                                                                                                                          cAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATAAAg        <  4:307707/149‑1 (MQ=255)
                                                                                                                                              gCGACCTGGTGGCCTTGCTCAAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACGGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCATTACGGGAAGGTGCTCATAACGAAgg    >  2:241257/1‑149 (MQ=255)
                                                                                                                                                gACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATAAAGCAGGgc  <  7:162283/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CAGGCCTCCCCAGGCCTGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATAAAGCAGGGC  >  NC_002947/3121216‑3121508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑