Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,121,368 G→C 7.8% H26D (CAC→GAC)  PP_2737 ← short‑chain dehydrogenase/reductase family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,121,3680GC 7.8% 153.2 / 3.8 51H26D (CAC→GAC) PP_2737short‑chain dehydrogenase/reductase family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (21/26);  new base C (2/2);  total (23/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01

CTCCCCAGGCCTGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATAAAGCAGGGC  >  NC_002947/3121221‑3121508
                                                                                                                                                   |                                                                                                                                            
cTCCCCAGGCCTGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGTGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCtggtgg                                                                                                                                             >  1:189391/1‑149 (MQ=255)
 tCCCCAGGCCTGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCCAGGCGACCTGGTGGc                                                                                                                                            >  4:254028/1‑149 (MQ=255)
  ccccAGGCCTGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCCACCAGGTCGcc                                                                                                                                           >  6:153949/1‑149 (MQ=255)
   cccAGGCCTGCTCGATATGCGCAGCGATGCCGGCTACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCt                                                                                                                                          <  7:321789/149‑1 (MQ=255)
   cccAGGCCTGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGc                                                                                                                                            <  7:180720/147‑1 (MQ=255)
     cAGGCCTGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCCACCAGGTCGCCTTg                                                                                                                                        <  5:153949/149‑1 (MQ=255)
         ccTGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTc                                                                                                                                     >  1:201787/1‑148 (MQ=255)
           tGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTcc                                                                                                                                    <  8:42368/147‑1 (MQ=255)
           tGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTcc                                                                                                                                    >  7:42368/1‑147 (MQ=255)
             cTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCagga                                                                                                                                <  1:232309/149‑1 (MQ=255)
                   atGCGCATCGATGCCGGCGACCTTCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCGTGCCCGGGAAACCGTTCTGACAGCGGGGCATGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACt                                                                                                                           <  5:68478/148‑1 (MQ=255)
                     gcgcAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCtt                                                                                                                                         <  7:14956/132‑1 (MQ=255)
                     gcgcAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCtt                                                                                                                                         >  8:14956/1‑132 (MQ=255)
                          gCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCACGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAg                                                                                                                   >  3:224800/1‑149 (MQ=255)
                             aTGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGAc                                                                                                                            <  6:244329/137‑1 (MQ=255)
                             aTGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGAc                                                                                                                            >  5:244329/1‑137 (MQ=255)
                                 cGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCa                                                                                                            >  3:282023/1‑149 (MQ=255)
                                  ggCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCAc                                                                                                           <  6:291803/149‑1 (MQ=255)
                                      aCCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCTGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGa                                                                                                        >  8:162283/1‑148 (MQ=255)
                                                  ttGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGgc                                                                                           >  8:235059/1‑149 (MQ=255)
                                                     tCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGcc                                                                                        >  1:8504/1‑149 (MQ=255)
                                                      cAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCg                                                                                       >  1:118811/1‑149 (MQ=255)
                                                      cAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCg                                                                                       >  5:78446/1‑149 (MQ=255)
                                                          gtCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCa                                                                                   <  2:132091/148‑1 (MQ=255)
                                                          aTCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCagga                                                                                                                                >  2:286473/1‑104 (MQ=255)
                                                             gCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGc                                                                                 <  2:8504/148‑1 (MQ=255)
                                                                aaTGGCCAGCAATGCCTGCCCGGGAAACCGTTCAGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCAGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTTCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCa                                                                                   >  7:159462/1‑143 (MQ=255)
                                                                aaTGGCCAGCAATGACTGCCCGGGAATCCTTTCTGCCAGCGGGGCAAGGCGTTCTGCGTTGCGACCGCCAAAGGCGACCTGGTGGCCTTTCTCCAGTAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCTGTCa                                                                                   <  8:159462/143‑1 (MQ=255)
                                                                     ccAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCgg                                                                        >  2:190724/1‑149 (MQ=255)
                                                                      cAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGc                                                                       >  3:307707/1‑149 (MQ=255)
                                                                        gCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAgc                                                                                                                  >  5:72113/1‑104 (MQ=255)
                                                                        gCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAgc                                                                                                                  <  6:72113/104‑1 (MQ=255)
                                                                               ctgccCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAg                                                              >  7:324489/1‑149 (MQ=255)
                                                                                  cccGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCTGCTCATGCCAgcct                                                           <  2:189391/149‑1 (MQ=255)
                                                                                  cccGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAgcc                                                            <  1:317749/148‑1 (MQ=255)
                                                                                    cGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAgcctgc                                                         >  5:280468/1‑149 (MQ=255)
                                                                                       gAAACCGTTCTGCCAGCGGGGCAATCCGGTCGGCGTTGAGCCCGACCAAGGCCACCAGGTCGCCTTgg                                                                                                                                       >  2:63058/1‑67 (MQ=21)
                                                                                       gAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCCACCAGGTCGCCTTgg                                                                                                                                       <  1:63058/68‑2 (MQ=38)
                                                                                         aaCCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGc                                                                          <  2:354267/127‑1 (MQ=255)
                                                                                          aCCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGa                                                   <  7:235059/149‑1 (MQ=255)
                                                                                             gTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGc                                                 >  7:145127/1‑148 (MQ=255)
                                                                                                      gCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTg                                       <  1:190724/149‑1 (MQ=255)
                                                                                                        ggggCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCt                                     <  6:69739/149‑1 (MQ=255)
                                                                                                          ggCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCtgt                                   <  8:145127/149‑1 (MQ=255)
                                                                                                           tcAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCtgtg                                  <  4:282023/148‑1 (MQ=255)
                                                                                                                gCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCAt                             <  6:280468/149‑1 (MQ=255)
                                                                                                                   gTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGGCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCATGAc                          <  4:224800/149‑1 (MQ=255)
                                                                                                                    tCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCATGACg                         <  6:78446/149‑1 (MQ=255)
                                                                                                                      ggCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCATGACggg                       <  2:118811/149‑1 (MQ=255)
                                                                                                                                     cAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATAAAg        <  4:307707/149‑1 (MQ=255)
                                                                                                                                         gCGACCTGGTGGCCTTGCTCAAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACGGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCATTACGGGAAGGTGCTCATAACGAAgg    >  2:241257/1‑149 (MQ=255)
                                                                                                                                           gACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATAAAGCAGGgc  <  7:162283/149‑1 (MQ=255)
                                                                                                                                                ggtggCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATaaa         <  5:257312/137‑1 (MQ=255)
                                                                                                                                                ggtggCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATaaa         >  6:257312/1‑137 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
CTCCCCAGGCCTGCTCGATATGCGCAGCGATGCCGGCGACCTGCTCCGGGTTGTCAAGATCGCCAATGGCCAGCAATGCCTGCCCGGGAAACCGTTCTGCCAGCGGGGCAAGGCGGTCGGCGTTGCGCCCGCCCAAGGCGACCTGGTGGCCTTGCTCCAGGAGGACTTGCGCGAGCGCGGCACCGATACCACTGCTGGCGCCGGTCAGCCAACAGCGGCTCATGCCAGCCTGCCTTTGAGCCAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATAAAGCAGGGC  >  NC_002947/3121221‑3121508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑