| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,208,521 | G→T | 7.0% | V724F (GTC→TTC) | PP_2812 → | transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,208,521 | 0 | G | T | 7.0% | 183.4 / 3.8 | 57 | V724F (GTC→TTC) | PP_2812 | transporter |
| Reads supporting (aligned to +/- strand): ref base G (25/28); new base T (2/2); total (27/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
GTTCATGAAGGAACTGCAGATCGGCCTGACCATTGCTACCTTGCCGGTGATGGTGTTGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTC > NC_002947/3208376‑3208661 | gTTCATGAAGGAACTGCAGATCGGCCTGACCATTGCTACCTTGCCGGTGATGGTGTTGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCTACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGTTc > 5:310657/1‑148 (MQ=255)gTTCATGAAGGAACTGCAGATCGGCCTGACCATTGCTACCTTGCCGGTGATGGTGTTGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGTTc > 5:146284/1‑148 (MQ=255)gTTCATGAAGGAACTGCAGATCGGCCTGACCATTGCTACCTTGCCGGTGATGGTGTTGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTc > 7:102016/1‑148 (MQ=255)gTTCATGAAGGAACTGCAGATCGGCCTGACCATTGCTACCTTGCCGGTGATGGTGTTGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCg > 2:48772/1‑149 (MQ=255) tGAAGGAACTGCAGATCGGCCTGACCATTGCTACCTTGCCGGTGATGGTGTTGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCAc < 4:245715/149‑1 (MQ=255) tGAAGGAACTGCAGATCGGCCTGACCATTGCTACCTTGCCGGTGATGGTGTTGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCAc < 7:86613/149‑1 (MQ=255) ggAACTGCAGATCGGCCTGACCATTGCTACCTTGCCGGTGATGGTGTTGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCAt > 3:113012/1‑149 (MQ=255) ggAACTGCAGATCGGCCTGACCATTGCTACCTTGCCGGTGATGGTGTTGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCAt > 8:210675/1‑149 (MQ=255) cTGCAGATCGGCCTGACCATTGCTACCTTGCCGCTCATGGTGTTGGCCGTGGGCATTGCTGTCGACTACGCCTTCTACATCTACAACCGGCTGCTACTGCACCTGGCCCACGGCCAGTCGAAAACCAAGGCGGTACCGCACCCATTGCt > 6:295701/1‑149 (MQ=255) ggCCTGACCATTGCTACCTTGCCGGTGATGGTGTTGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTgg < 4:108827/148‑1 (MQ=255) ggCCTGACCATTGCTACCTTGCCGGTGATGGTGTTGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTgg < 4:103931/148‑1 (MQ=255) cATTGCTACCTTGCCGGTGATGGTGTTGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGcga < 8:152243/149‑1 (MQ=255) cTACCTTGCCGGTGATGGTGTTGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCgg < 5:7223/111‑1 (MQ=255) cTACCTTGCCGGTGATGGTGTTGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCgg > 6:7223/1‑111 (MQ=255) tACCTTGCCGGTGATGGTGTTGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCt > 7:266508/1‑149 (MQ=255) gCCGGTGATGGTGTTGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCAcc < 5:17436/148‑1 (MQ=255) gTGATGGTGTTGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGACCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTTGGCGTTGAGACGATCTTCACAGCCAt > 6:253984/1‑149 (MQ=255) gATGGTGTTGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGGTGCGACGCGCTTCACCGCCATTa > 8:228659/1‑149 (MQ=255) ttGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTgg < 1:48772/148‑1 (MQ=255) ttGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGc < 8:266508/149‑1 (MQ=255) ccGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGTCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTg < 1:245903/149‑1 (MQ=255) tGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGc < 1:338091/149‑1 (MQ=255) ggCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGt > 6:147992/1‑149 (MQ=255) gACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAg < 4:94022/90‑1 (MQ=255) gACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAg > 3:94022/1‑90 (MQ=255) gCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGTTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTtctc < 6:310657/149‑1 (MQ=255) gCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGTTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTtctc < 6:146284/149‑1 (MQ=255) ctacatctacaACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGa < 5:147992/147‑1 (MQ=255) acatctacaACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCg < 6:15707/143‑1 (MQ=255) acatctacaACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCg > 5:15707/1‑143 (MQ=255) catctacaACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTg < 8:82322/70‑1 (MQ=255) catctacaACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTg > 7:82322/1‑70 (MQ=255) catctacaACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTc < 7:210675/148‑1 (MQ=255) catctacaACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTc > 7:156654/1‑148 (MQ=255) caACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCAt < 1:39310/62‑1 (MQ=255) caACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCAt > 2:39310/1‑62 (MQ=255) caACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAg < 5:263546/145‑1 (MQ=255) aaCCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTc > 5:160560/1‑147 (MQ=255) aaCCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCa < 4:113012/149‑1 (MQ=255) aCCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAg < 8:8921/149‑1 (MQ=255) cTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGa > 7:111728/1‑125 (MQ=255) cTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGa < 8:111728/125‑1 (MQ=255) cTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATggg > 8:34530/1‑149 (MQ=255) gCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGc < 8:168358/105‑1 (MQ=255) cACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGc < 8:102016/147‑1 (MQ=255) aCCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAg > 8:210767/1‑149 (MQ=255) cccACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGctgctg > 8:314733/1‑149 (MQ=255) ccACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTa > 6:311149/1‑77 (MQ=255) ccACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTa < 5:311149/77‑1 (MQ=255) ccACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTgg > 6:66288/1‑149 (MQ=255) ccACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTgg < 5:253984/149‑1 (MQ=255) gCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTc < 7:314733/149‑1 (MQ=255) cAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCAt < 8:156654/149‑1 (MQ=255) ccAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGt > 6:14274/1‑148 (MQ=255) ccAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGt > 6:237660/1‑148 (MQ=255) ccAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTc < 7:34530/149‑1 (MQ=255) cAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTc > 3:183327/1‑136 (MQ=255) cAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTc < 4:183327/136‑1 (MQ=255) cAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTc > 3:222616/1‑148 (MQ=255) | GTTCATGAAGGAACTGCAGATCGGCCTGACCATTGCTACCTTGCCGGTGATGGTGTTGGCCGTGGGCATTGGTGTCGACTACGCCTTCTACATCTACAACCGGCTGCAACTGCACCTGGCCCACGGCCAGTCGATCACCAAGGCGGTCGAGCACGCATTGCTCGAGGTGGGCGTTGCGACGATCTTCACCGCCATTACCCTGGCGGTGGGCGTGGCTACCTGGGCGTTCTCGGAGCTCAAGTTCCAGGCCGACATGGGCAAGCTGCTGGCCTTCATGTTCATCGTC > NC_002947/3208376‑3208661 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |