Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,233,690	T→C	5.9%	V293A (GTG→GCG)	GbpR →	galactose‑binding protein regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,233,690	0	T	C	5.9%	198.5 / 2.5	68	V293A (GTG→GCG)	GbpR	galactose‑binding protein regulator
Reads supporting (aligned to +/- strand):  ref base T (31/33);  new base C (2/2);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

CGCTTCGATCCTGGCGACCACTGCCATGCTCGAAGCGTCCGAAATGATTGCGGTGGTGCCCAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATGCCGCCGGACATGACCCATTCCAGCTTTTGCAACTGGATCCGCTACAA  >  NC_002947/3233544‑3233837                                                                                                                                                   |                                                                                                                                                     cGCTTCGATCCTGGCGACCACTGCCATGCTCGAAGCGTCCGAAATGATTGCGGTGGTGCCCAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGc                                                                                                                                                    <  1:306503/149‑1 (MQ=255) cGCTTCGATCCTGGCGACCACTGCCATGCTCGAAGCGTCCGAAATGATTGCGGTGGTGCCCAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGc                                                                                                                                                    >  6:345854/1‑149 (MQ=255)      cGATCCTGGCGACCACTGCCATGCTCGAAGCGTCCGAAACGATTGCGGTGGTGCCCAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGAcc                                                                                                                                               >  3:217379/1‑149 (MQ=255)       gATCCTGGCGACCACTGCCATGCTCGAAGCGTCCGAAATGATTGCGGTGGTGCCCAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGAcc                                                                                                                                               <  6:179761/148‑1 (MQ=255)                   cACTGCCATGCTCGAAGCGTCCGAAATGATTGCGGTGGTGCCCAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTcgcg                                                                                                                                   <  3:88640/147‑1 (MQ=255)                   cACTGCCATGCTCGAAGCGTCCGAAATGATTGCGGTGGTGCCCAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTcgcg                                                                                                                                   >  4:88640/1‑147 (MQ=255)                         cATGCTCGAAGCGTCCGAAATGATTGCGGTGGTGCCCAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACt                                                                                                                           >  3:148785/1‑149 (MQ=255)                         cATGCTCGAAGCGTCCGAAACGATTGCGGTGGTGCCCAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACt                                                                                                                           <  4:217379/149‑1 (MQ=255)                              tCGTAGCGTCCGAAATGATTGCGGTGGTGCCCAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGGCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGtt                                                                                                                      <  4:350447/149‑1 (MQ=255)                              tCGAAGCGTCCGAAATGATTGCGGTGGTGCCCAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGtt                                                                                                                      >  2:213077/1‑149 (MQ=255)                              tCGAAGCGGCCGAAATGATTGCGGTGGGGCGCAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGGCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTgc                                                                                                                                           <  5:72248/129‑1 (MQ=255)                                gAAGCGTCCGAAATGATTGCGGTGGTGCCCAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGtt                                                                                                                      <  1:213077/147‑1 (MQ=255)                                    cGTCCGAAATGATTGCGGTGGTGCCCAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCtt                                                                                                                <  1:334836/149‑1 (MQ=255)                                       ccGAAATGATTGCGGTGGTGCCCAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGa                                                                                                              >  1:327573/1‑148 (MQ=255)                                          aaaTGATTGCGGTGGTGCCCAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATAcc                                                                                                          >  6:261985/1‑149 (MQ=255)                                               aTTGCGGTGGTGCCCAATAACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTtgct                                                                                                     <  6:238747/149‑1 (MQ=255)                                                   cGGTGGTGCCCAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCgg                                                                                                                                  <  6:27569/116‑1 (MQ=255)                                                   cGGTGGTGCCCAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCgg                                                                                                                                  >  5:27569/1‑116 (MQ=255)                                                            ccAATGACGTGGCCCAGCATTATGACCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTCGCGATGGCCAACCTCGGCGTGCTGACCCTCCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCTTAAACTGCgg                                                                                        >  4:290952/1‑149 (MQ=255)                                                             cAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCggg                                                                                       >  5:80534/1‑149 (MQ=255)                                                                     tGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGcactctta                                                                               >  6:87654/1‑144 (MQ=255)                                                                        cccAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACtt                                                                            >  6:297981/1‑149 (MQ=255)                                                                              aTTATGCCCGCTATGGCATGGCCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGc                                                                                                                                                    >  5:369151/1‑72 (MQ=255)                                                                              aTTATGCCCGCTATGGCATGGCCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGc                                                                                                                                                    <  6:369151/72‑1 (MQ=255)                                                                               ttATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGTCACAATAACTTTCACCTg                                                                     <  5:345854/149‑1 (MQ=255)                                                                                  tGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCa                                                                   <  3:290952/148‑1 (MQ=255)                                                                                  tGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCAt                                                                  >  2:50029/1‑149 (MQ=255)                                                                                    cctgCTATGGCATGGTCGCCGTCCTGCCGGTGTAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGa                                                                <  8:163309/146‑1 (MQ=255)                                                                                    cccGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGa                                                                <  5:297981/149‑1 (MQ=255)                                                                                     ccGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAg                                                               >  2:345666/1‑149 (MQ=255)                                                                                       gCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGc                                                             >  4:230021/1‑149 (MQ=255)                                                                                           tGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGACCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTg                                                         >  6:3450/1‑149 (MQ=255)                                                                                             gCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGccc                                                                                                                               <  5:39557/77‑1 (MQ=255)                                                                                             gCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGccc                                                                                                                               >  6:39557/1‑77 (MQ=255)                                                                                              cATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGt                                                      >  3:109373/1‑149 (MQ=255)                                                                                              cATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGt                                                      <  1:345666/149‑1 (MQ=255)                                                                                               aTGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCGACTCGCGGCCCAACTCGGTTGCCCTTGATa                                                                                                            <  1:27389/95‑1 (MQ=255)                                                                                               aTGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATa                                                                                                            >  2:27389/1‑94 (MQ=255)                                                                                                tGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCt                                                    <  3:230021/149‑1 (MQ=255)                                                                                                  gTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTa                                                   <  6:80534/148‑1 (MQ=255)                                                                                                  gTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTAt                                                  <  2:327573/149‑1 (MQ=255)                                                                                                    cGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATgc                                                >  5:72160/1‑149 (MQ=255)                                                                                                    cGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGCGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATgc                                                >  7:41372/1‑149 (MQ=255)                                                                                                    cGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGCGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATgc                                                >  7:35790/1‑149 (MQ=255)                                                                                                     gCCGTCCTGCCGGTGGAGTTGCGACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATgcc                                               >  7:225760/1‑149 (MQ=255)                                                                                                     gCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATgcc                                               >  2:89953/1‑149 (MQ=255)                                                                                                     gCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATgc                                                <  4:109373/148‑1 (MQ=255)                                                                                                        gTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGa                                                                >  7:36054/1‑129 (MQ=255)                                                                                                        gTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGa                                                                <  8:36054/129‑1 (MQ=255)                                                                                                           cTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATGCCGCCGGa                                         >  2:16266/1‑149 (MQ=255)                                                                                                              ccGGTCGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATGCCGCCGGACAt                                      >  4:165933/1‑149 (MQ=255)                                                                                                                  tGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATGCCGCCGGACATGAcc                                  <  1:89953/149‑1 (MQ=255)                                                                                                                       ttGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCt                                                                      >  2:172769/1‑108 (MQ=255)                                                                                                                       ttGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCt                                                                      <  1:172769/108‑1 (MQ=255)                                                                                                                          ccACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATGccgccg                                           >  8:132983/1‑132 (MQ=255)                                                                                                                          ccACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATGccgccg                                           <  7:132983/132‑1 (MQ=255)                                                                                                                            aCTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATGCCGCCGGAc                                        >  4:344929/1‑133 (MQ=255)                                                                                                                            aCTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATGCCGCCGGAc                                        <  3:344929/133‑1 (MQ=255)                                                                                                                            aCTGGAGATGGCCAACCTCGGCGTGCTGAACCTGCG‑CTCGCGGCCCAACTAGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGAATGAGGCCCTGCGTCTATGCCGCCGGACATGACGCATTCCAGCt                        >  5:269384/1‑149 (MQ=255)                                                                                                                                gCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATGCCGCCGGACATGACCCATTCCAGCttt                      <  4:148785/147‑1 (MQ=255)                                                                                                                                gCGATGGCCAACCTCGGCGCGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATGCCGCCGGACATGACCCATTCCAGCttt                      <  8:35790/147‑1 (MQ=255)                                                                                                                                gCGATGGCCAACCTCGGCGCGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATGCCGCCGGACATGACCCATTCCAGCttt                      <  8:41372/147‑1 (MQ=255)                                                                                                                                 cGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATGCCGCCGGACATGACCCATTCCAGCTTTTGc                   <  7:268327/149‑1 (MQ=255)                                                                                                                                  gATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATGCCGCCGGACATGACCCATTCCAGCTTTTGc                   >  1:20179/1‑148 (MQ=255)                                                                                                                                   aTGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATGCCGCCGGACATGACCCATTCCAGCTTTTGCaa                 <  1:50029/149‑1 (MQ=255)                                                                                                                                    tGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATGCCGCCGGACATGACCCATTCCAGCTTTTGCAAc                <  2:20179/149‑1 (MQ=255)                                                                                                                                      gCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATGCCGCCGGACATGACCCATTCCAGCTTTTGCAACt               <  3:165933/148‑1 (MQ=255)                                                                                                                                                  gTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATGCCGCCGGACATGACCCATTCCAGCTTTTGCAACTGGATCCGCTACaa  <  5:261985/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     CGCTTCGATCCTGGCGACCACTGCCATGCTCGAAGCGTCCGAAATGATTGCGGTGGTGCCCAATGACGTGGCCCAGCATTATGCCCGCTATGGCATGGTCGCCGTCCTGCCGGTGGAGTTGCCACTGGCGATGGCCAACCTCGGCGTGCTGACCCTGCG‑CTCGCGGCCCAACTCGGTTGCCCTTGATACCTTGCTGCGTTACCTGCGGGCACAATAACTTTCACCTGCATGAGGCCCTGCGTCTATGCCGCCGGACATGACCCATTCCAGCTTTTGCAACTGGATCCGCTACAA  >  NC_002947/3233544‑3233837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑