Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,258,718 A→C 7.7% intergenic (‑40/+22) PP_2855 ← / ← PP_2856 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,258,7180AC 7.7% 147.0 / 4.8 52intergenic (‑40/+22)PP_2855/PP_2856hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/23);  new base C (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

GGTTTGAACACCACGGCCCCGCCCGGCAGGACGGACAGCGGCATTTGTGCAGCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGGCTGCTACGTTCACGCCCAGGTTACCGCTGCCACCGTTCACCGAACCGCTAAGGGTTGCAGTGTTGGTAAC  >  NC_002947/3258575‑3258865
                                                                                                                                               |                                                                                                                                                    
gggttGATCACCACGGCGCCGCCCGGCTGGACGGACAGCGGCATTTGTGCAGCTTCGCTCACGCTGGCCAGGCTCAGCAAAAACGCCAAGGGGAAAATACGCATGATCGGATGCCTTACGGGGTGTTGATGAATCGCCCCCCGAAgggg                                                                                                                                                 <  6:150586/146‑1 (MQ=255)
ggTTTGAACACCACGGCCCCGCCCGGCAGGACGGACAGCGGCATTTGTGCAGCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAgggg                                                                                                                                                 <  2:265501/149‑1 (MQ=255)
  tttGAACACCACGGCCCCGCCCGGCAGGACGGACAGCGGCATTTGTGCAGCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAgggg                                                                                                                                                 <  1:197791/147‑1 (MQ=255)
  tttGAACACCACGGCCCCGCCCGGCAGGACGGACAGCGGCATTTGTGCAGCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAgggg                                                                                                                                                 >  2:197791/1‑147 (MQ=255)
  tttGAACACCACGGCCCCGCCCGGCAGGACGGACAGCGGCATTTGCGCAGCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGc                                                                                                                                               <  4:323735/149‑1 (MQ=255)
     gAACACCACGGCCCCGCCCGGCAGGACGGACAGCGGCATTTGTGCAGCTTCGCTCACGGTGGCCAGGCACAGCAAAAACGCCAAGGCGAAATTTCGGATGTTCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCACCGAAgtgtgcat                                                                                                                                             >  4:33760/1‑141 (MQ=255)
      aaCACCACGGCCCCGCCCGGCAGGACGGACAGCGGCATTTGTGCAGCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTc                                                                                                                                            <  2:257064/148‑1 (MQ=255)
       acacCACGGCCCCGCCCGGCAGGACGGACAGCGGCATTTGTGCAGCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCaa                                                                                                                                          <  3:200297/149‑1 (MQ=255)
           cacGGCCCCGCCCGGCAGGACGGACAGCGGCATTTGTGCAGCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGGATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGca                                                                                                                                       >  6:37052/1‑148 (MQ=255)
                      ccgGCAGGACGGACAGCGGCATTTGTGCAGCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGaa                                                                                                                           <  3:6756/149‑1 (MQ=255)
                          cAGGACGGACAGCGGCATTTGTGCAGCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGACTAACTGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGaa                                                                                                                       <  3:33760/149‑1 (MQ=255)
                              acggacAGCGGCATTTGTGCAGCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAgtgt                                                                                                                   <  7:100309/149‑1 (MQ=255)
                               cggacAGCGGCATTTGTGCAGCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAATAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGtt                                                                                                                  <  3:105028/149‑1 (MQ=255)
                                ggacAGCGGCATTTGTGCAGCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTg                                                                                                                 <  6:129302/149‑1 (MQ=255)
                                ggacAGCGGCATTTGTGCAGCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTg                                                                                                                 <  3:295272/149‑1 (MQ=255)
                                       ggCATTTGTGCAGCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATgg                                                                                                          >  7:285178/1‑149 (MQ=255)
                                         cATTTGTGCAGCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGaa                                                                                                                       <  8:223831/134‑1 (MQ=255)
                                         cATTTGTGCAGCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGaa                                                                                                                       >  7:223831/1‑134 (MQ=255)
                                           tttGTGCAGCTTCGGTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGACTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAg                                                                                                      >  7:307204/1‑149 (MQ=255)
                                              gtgCAGCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCGTCAAGCACAGCGGGCAGAACGAAGTGTGGGAGATGGCCAGc                                                                                                     >  2:184980/1‑147 (MQ=255)
                                                   gCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCTTCGGGGGGCCtttc                                                                                                                                          <  5:223006/105‑4 (MQ=255)
                                                   gCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCTTCGGGGGGCCtttc                                                                                                                                          >  6:223006/1‑102 (MQ=255)
                                                         cTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTgg                                                                                                                <  8:240452/125‑1 (MQ=255)
                                                         cTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTgg                                                                                                                >  7:240452/1‑125 (MQ=255)
                                                          tCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAACAGATGGAGATGGCCAGCGAGttgctttgctc                                                                                       >  6:164159/1‑148 (MQ=255)
                                                            aCGCTGGCCAGGCAAAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTg                                                                                       <  5:164159/147‑1 (MQ=255)
                                                                tGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGcc                                                                                >  8:118964/1‑149 (MQ=255)
                                                                  gCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCca                                                                              >  4:118455/1‑149 (MQ=255)
                                                                     aGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCacac                                                                           >  1:248140/1‑149 (MQ=255)
                                                                         acaGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGgc                                                                       >  8:85802/1‑149 (MQ=255)
                                                                                aaaCGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCTTCGGGGGGCCt                                                                                                                                             <  8:275892/73‑1 (MQ=38)
                                                                                aaaCGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCTTCGGGGGGCCt                                                                                                                                             >  7:275892/1‑73 (MQ=38)
                                                                                 aaCGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGGCTGCTACGt                                                               >  8:12237/1‑149 (MQ=255)
                                                                                          gCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGGCTGCTACGTTCACGCCCa                                                      <  8:285178/149‑1 (MQ=255)
                                                                                                      aTGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGGCTGCTACGTTCACGCCCAGGTTACCGCTGc                                          >  6:59789/1‑149 (MQ=255)
                                                                                                      aTGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGGCTGCTACGTTCACGCCCAGGTTACCGCTGc                                          <  3:118455/149‑1 (MQ=255)
                                                                                                            ggATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCGTTGCTGG‑TTGCCCACACCGGCTGCTACGTTCACGCCCAGGTTACCGCTGCCACCGt                                    >  4:245187/1‑149 (MQ=255)
                                                                                                            ggATGCCTTACGGGGTGTTGCACAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGGCTGCTACGTTCACGCCCAGGTTACCGCTGCCACCGt                                    <  1:184980/149‑1 (MQ=255)
                                                                                                             gATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGGCTGCTACGTTCACGCCCAGGTTACCGCTGCCACCGtt                                   >  5:366303/1‑149 (MQ=255)
                                                                                                                gCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGGCTGCTACGTTCACGCCCAGGTTACCGCTGCCACCGTTCAc                                >  4:11503/1‑149 (MQ=255)
                                                                                                                 ccTTACGGTGTGGTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGCTGGCCAGCGAGTTGCTTTGCTGGCTTGCCCACACCGGCTGCTACGTTCACGCCCAGGTTACCGCTGCCACCGTTCAc                                <  1:273896/149‑1 (MQ=255)
                                                                                                                  cTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGGCTGCTACGTTCACGCCCAGGTTACCGCTGCCACCGTTCAcc                               >  1:60631/1‑148 (MQ=255)
                                                                                                                   ttACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCAAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGGCTGCTACGTTCACGCCCAGGTTACCGCTGCCACCGTTCACCg                              >  3:1611/1‑148 (MQ=255)
                                                                                                                       ggTGTGTTGAAGAACGGCCCCCCGAAGTGTGCCTGAAGGACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGGGAGTTGCTTTGCTGG‑TTGCGCTCACCGGCTGCTACGTTCACGGCATGGTTACCGGTGCCACCGTTCACCGAACCg                         <  8:307204/149‑1 (MQ=255)
                                                                                                                         tgtgtTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGGCTGCTACGTTCACGCCCAGGTTACCGCTGCCACCGTTCACCGAACCGc                        >  6:228557/1‑148 (MQ=255)
                                                                                                                             ttGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGGCTGCTACGTTCACGCCCAGGTTACCGCTGCCACCGTTCACCGAACCGCTAAgg                   >  7:207336/1‑149 (MQ=255)
                                                                                                                                aagaaAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGGCTGCTACGTTCACGCCCAGGTTACCGCTGCCACCGTTCACCGAACCGCTAAGGGtt                >  1:282481/1‑149 (MQ=255)
                                                                                                                                  gaaAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGGCTGCTACGTTCACGCCCAGGTTACCGCTGCCACCGTTCCCCGAACCGCTAAGGGTTg               >  7:314639/1‑148 (MQ=255)
                                                                                                                                         cccccGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGGCTGCTACGTTCACGCCCAGGTTACCGCTGCCACCGTTCACCGAACCGCTAAGGGTTGCAGTGTTg       <  7:85802/149‑1 (MQ=255)
                                                                                                                                           cccGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGGCTGCTACGTTCACGCCCAGGTTACCGCTGCCACCGTTCACCGAACCGCTAAGGGTTGCAGTGTTGGt     <  5:59789/149‑1 (MQ=255)
                                                                                                                                             cGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGGCTGCTACGTTCACGCCCAGGTTACCGCTGCCACCGTTCACCGAACCGCTAAGGGTTGCAGTGTTGGTaa   >  7:51211/1‑149 (MQ=255)
                                                                                                                                              gAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGGCTGCTACGTTCACGCCCAGGTTACCGCTGCCACCGTTCACCGAACCGCTAAGGGTTGCAGTGTTGGTAAc  <  7:118964/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
GGTTTGAACACCACGGCCCCGCCCGGCAGGACGGACAGCGGCATTTGTGCAGCTTCGCTCACGCTGGCCAGGCACAGCAAAAACGCCAAGGCGAAAATACGCATGATCGGATGCCTTACGGTGTGTTGAAGAAAGGCCCCCCGAAGGGGGCCTCAAGCACAGCGGTCAGAACGAAGTGTTGGAGATGGCCAGCGAGTTGCTTTGCTGG‑TTGCCCACACCGGCTGCTACGTTCACGCCCAGGTTACCGCTGCCACCGTTCACCGAACCGCTAAGGGTTGCAGTGTTGGTAAC  >  NC_002947/3258575‑3258865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑