Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,296,502 G→A 8.5% Q125* (CAG→TAG) ‡ PP_2898 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,296,5020GA 8.5% 132.2 / 2.2 48Q125* (CAG→TAG) ‡PP_2898hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (22/21);  new base A (2/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.12e-01

CGATGATGTGCAGTGGGTACAGCACCCCTCAATCGCACGGGTAGCTGCTCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGCGGGCTGTGGGCCCGCTGAAATGTA  >  NC_002947/3296355‑3296645
                                                                                                                                                    |                                                                                                                                               
cgATGATGTGCAGTGGGTACAGCACCCCTCAATCGCACGGGTAGCTGCTCAGCAAACTGGCGCTACCCAGATAGGCTTTAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTgg                                                                                                                                                <  4:147643/149‑1 (MQ=255)
         gCAGTGGGTACAGCACCCCTCAATCGCACGGGTAGCTGCTCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTgcgc                                                                                                                                       >  6:309597/1‑149 (MQ=255)
          cAGTGGGTACAGCACCCCTCAATCGCACGGGTAGCTGCTCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTgcgc                                                                                                                                       <  7:205329/148‑1 (MQ=255)
                   cAGCACCCCTCAATCGCACGGGTAGCCGCTCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCGACGTGCAACGGCTGGCCGTTGCGCTGGCCGAt                                                                                                                               <  1:38582/148‑1 (MQ=255)
                      cACCCCTCAATCGCACGGGTAGCTGCTCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTgac                                                                                                                                   >  7:75701/1‑138 (MQ=255)
                      aaCCCCTCAATCGCACGGGTAGCTGCTCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTgac                                                                                                                                   <  8:75701/139‑3 (MQ=255)
                       aCCCCTCAATCGCACGGGTAGCTGCTCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTg                                                                                                                         <  4:266046/149‑1 (MQ=255)
                           cTCAATCGCACGGGTAGCTGCTCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCg                                                                                                                     >  1:148125/1‑149 (MQ=255)
                            tCAATCGCACGGGTAGCTGCTCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCgg                                                                                                                    >  8:77613/1‑149 (MQ=255)
                              aaTCGCACGGGTAGCTGCTCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATTGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGc                                                                                                                   >  3:163332/1‑148 (MQ=255)
                                            ctgctCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATTGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTa                                                                                                    <  4:163332/149‑1 (MQ=255)
                                              gctCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTa                                                                                                    >  5:225135/1‑147 (MQ=255)
                                              gctCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTa                                                                                                    >  6:286862/1‑147 (MQ=255)
                                               ctCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCCCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAg                                                                                                 <  5:309597/149‑1 (MQ=255)
                                                tCAGCAAACTGGCGCTACCCGGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGCGCAACGGCCAGCCGTTgca                                                                                                                                        >  3:91210/1‑108 (MQ=255)
                                                tCAGCAAACTGGCGCTACCCGGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGCGCAACGGCCAGCCGTTgca                                                                                                                                        <  4:91210/109‑2 (MQ=255)
                                                      aaCTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTgg                                                                                          <  2:121474/149‑1 (MQ=255)
                                                           gcgcTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTc                                                                                       <  1:205873/147‑1 (MQ=255)
                                                            cgcTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGa                                                                                    <  5:286862/149‑1 (MQ=255)
                                                            cgcTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGGCTGa                                                                                    <  7:86270/149‑1 (MQ=255)
                                                                  ccAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGt                                                                              <  8:80628/149‑1 (MQ=255)
                                                                  ccAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGt                                                                              >  6:81966/1‑149 (MQ=255)
                                                                   cAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTg                                                                             >  8:335733/1‑149 (MQ=255)
                                                                     gATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGc                                                                           >  1:150549/1‑149 (MQ=255)
                                                                     gATACGCTTGAGGGGTTGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACAAGCAGCCACACCG‑CGTGCAACGGCTCGCCGATGCGCTGGCCGATGACCTGATCGGCAAAGTTGCGCAGGTACAGTACCTCGGTCTGATTCACTGGc                                                                           >  8:75779/1‑149 (MQ=255)
                                                                      aTAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCt                                                                          >  7:309645/1‑149 (MQ=255)
                                                                      aTAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCt                                                                          >  4:278343/1‑149 (MQ=255)
                                                                             tGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGgcggcg                                                                                                       <  2:144782/113‑1 (MQ=255)
                                                                             tGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGgcggcg                                                                                                       >  1:144782/1‑113 (MQ=255)
                                                                               aGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGt                                                                 <  7:77613/149‑1 (MQ=255)
                                                                                ggggTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTc                                                                <  5:81966/149‑1 (MQ=255)
                                                                                  ggTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGAtt                                                                                  <  6:329437/129‑1 (MQ=255)
                                                                                  ggTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGAtt                                                                                  >  5:329437/1‑129 (MQ=255)
                                                                                                           gTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAg                                     <  6:225135/149‑1 (MQ=255)
                                                                                                           gTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAg                                     <  2:148125/149‑1 (MQ=255)
                                                                                                           gTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAg                                     >  2:343921/1‑149 (MQ=255)
                                                                                                             gcgcCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGAGCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGttt                                               >  6:153633/1‑137 (MQ=255)
                                                                                                             gcgcCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGAGCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGttt                                               <  5:153633/137‑1 (MQ=255)
                                                                                                              cgcCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGt                                   <  3:149014/148‑1 (MQ=255)
                                                                                                               gcCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCg                                 >  3:33674/1‑149 (MQ=255)
                                                                                                                  aaTCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGc                              <  7:335733/149‑1 (MQ=255)
                                                                                                                  aaTCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCAAGGTCggg                               >  4:226699/1‑148 (MQ=255)
                                                                                                                         cagcagGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGCggg                       >  6:89106/1‑149 (MQ=255)
                                                                                                                                cacaGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGCGGGCTGTggg                >  2:257848/1‑149 (MQ=255)
                                                                                                                                 acaGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGCGGGCTGTGGGc               >  7:107968/1‑149 (MQ=255)
                                                                                                                                     gccagcgCAACGGCCAGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGCGGGCTGTgg                 <  3:103974/138‑1 (MQ=255)
                                                                                                                                     gccagcgCAACGGCCAGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGCGGGCTGTgg                 >  4:103974/7‑144 (MQ=255)
                                                                                                                                         gTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGGACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGCGGGCTGTGGGCCCGCTg         >  2:93341/1‑148 (MQ=255)
                                                                                                                                               cGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGCGGGCTGTGGGCCCGCTGAAATGTa  <  7:86975/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CGATGATGTGCAGTGGGTACAGCACCCCTCAATCGCACGGGTAGCTGCTCAGCAAACTGGCGCTACCCAGATAGGCTTGAGGGGTGGCGCGCATGCTCGGCGGCAACGTGCGCCAATCGACCAGCAGGCACAGCG‑CGTGCAACGGCTGGCCGTTGCGCTGGCCGATGACCTGGTCGGCAAAGTGGCGGCGGTACAGTACCTGGGTCTGATTCAGTGGCTGTGCCTGGTCGGCCTGCGCCAGCGTTTGTCGGGCCAGGTCGGGCAAGCGGGCTGTGGGCCCGCTGAAATGTA  >  NC_002947/3296355‑3296645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑