| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,339,218 | A→G | 9.7% | intergenic (‑220/+23) | PP_2935 ← / ← ybiT | membrane protein/ABC‑F family ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,339,218 | 0 | A | G | 9.7% | 108.9 / 2.7 | 43 | intergenic (‑220/+23) | PP_2935/ybiT | membrane protein/ABC‑F family ATPase |
| Reads supporting (aligned to +/- strand): ref base A (24/14); new base G (2/2); total (27/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
CGAATTATTCGTCGGGCCGTGCCGGTCGGTGGGATGGGGATGTACTCTTCGCGGGCTTGCCCGCTCTCCCAAGGCCCCACAAGCGTCCAATACCTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCGATGATGCGCGTGGCCAGCGACGACACGAACTCGCGGTCGTGGCTGA > NC_002947/3339073‑3339365 | cGAATTATTCGTCGGGCCGTGCCGGTCGGTGGGATGGGGATGTACTCTTCGCGGGCTTGCCCGCTCTCCCAAGGCCCCACAAGCGTCCAATACCTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCCTGCGAGAt > 6:94260/1‑149 (MQ=255) ttCGTCGGGCCGTGCCGGTCGGTGGGATGGGGATGTACTCTTCGCGGGCTTGCCCGCTCTCCCAAGGCCCCACAAGCGTCCAATACCTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCTGGCCATCTCGGATGGCCggg > 7:307759/1‑149 (MQ=39) cgtcgGGCCGTGCCGGTCGGTGGGATGGGGATGTACTCTTCGCGGGCTTGCCCGCTCTCCCAAGGCCCCACAAGCGTCCAATACCTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCTGGCCATCTCGGATGGCCGGGc < 8:307759/148‑1 (MQ=39) cgGGCCGTGCCGGTCGGTGGGAGGGGGATGTACTCTTCGCGGGCTTGCCCGCTCTCCCACGGCCCCACAAGCGTCCAATACCTGTGCGGTCCCTGGGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑c < 1:302210/149‑1 (MQ=255) gCCGTGCCGGTCGGTGGGATGGGGATGTACTCTTCGCGGGCTTGCCCGCTCTCCCAAGGCCCCACAAGCGTCCAATACCTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCCTCCGACATGGGCCGGGC‑‑GG‑ccc > 2:119765/1‑148 (MQ=38) gTGCCGGGCGGTGGGATGGGGATGTACTCTTCGCGGGCTTGCCCGCTCTCCCACGGCCCCACAAGCGTCCAATACCTGTGCGGGCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCatca < 2:120293/149‑1 (MQ=255) cggtGGGATGGGGATGTACTCTTCGCGGGCTTGCCCGCTCTCCCAAGGCCCCACAAGCGTCCAATACCTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGa < 4:229223/147‑1 (MQ=255) cggtGGGATGGGGATGTACTCTTCGCGGGCTTGCCCGCTCTCCCAAGGCCCCACAAGCGTCCAATACCTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGa > 3:229223/1‑147 (MQ=255) gtGGGATGGGGATGTACTCTTCGCGGGCTTGCCCGCTCTCCCAAGGCCCCACAAGCGTCCAATACCTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAg < 6:138192/149‑1 (MQ=255) gatgTACTCTTCGCGGGCTTGCCCGCTCTCCAAAGGCCCCACAAGCGTCCAATACCTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTgg < 3:229100/149‑1 (MQ=255) ttCGCGGGCTTGCCCGCTCTCCCAAGGCCCCACAAGCGTCCAATACCTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCa < 6:144954/147‑1 (MQ=255) cgcgGGCTTGCCCGCTCTCCCAAGGCCCCACAAGCGTCCAATACCTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAgg > 6:99664/1‑147 (MQ=255) cccGCTCTCCCAAGGCCCCACAAGCGTCCAATACCTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACAAGCACACCCTGGCTAAGCAGGTAGTCATCGTAg > 2:55887/1‑149 (MQ=255) ccAAGGCCCCACAAGCGTCCAATACCTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGTTGGGGTCTGTCCCAGTGAACAAAAATCAGACCAGCCCACCCAGGCTACGCAGGTAGTCATCGTAGCTa > 1:296741/1‑146 (MQ=19) ccAAGGCCCCACAAGCGTCCAATACCTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGt < 8:207108/129‑1 (MQ=255) ccAAGGCCCCACAAGCGTCCAATACCTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGt > 7:207108/1‑129 (MQ=255) cAAGGCCCCACAAGCGTCCAATACCTGTGCGCTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCACGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTg > 3:62155/1‑148 (MQ=255) cacaAGCGTCCAATACCTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTccac > 1:232116/1‑148 (MQ=255) cAATACCTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCgg > 3:181898/1‑149 (MQ=255) aaTACCTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGc > 1:230634/1‑149 (MQ=255) ccTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGa > 3:9755/1‑148 (MQ=255) cTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGa > 6:79433/1‑147 (MQ=255) gTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCgat > 3:305136/1‑149 (MQ=255) tCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCgat > 1:331188/1‑148 (MQ=255) ggAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCGATGATGCGCGt < 2:296741/149‑1 (MQ=255) gcccggggAAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCGATGATGCGCGt < 1:166177/144‑1 (MQ=255) gCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCCACAGCTCGATGATGCGCGTGGc > 6:208748/1‑149 (MQ=255) gCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCGATGATGCGCGTGGCCAGc > 5:258942/1‑149 (MQ=255) aaGCCCGCGAAGAGGCCAGGCCATCCGAGATGGCCAGGCC‑‑GT‑CACATCAATCAGACCAGCACACACTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCAACAACACCATCGGCCGACAGCTCGATGATGCGCGTGGCCAGcga > 1:66141/1‑149 (MQ=255) ccGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCGATGATGCGCGTGGCCAGcgacga > 2:233482/1‑148 (MQ=255) gCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCGATGATGCGCGTGGCCAGCGACGACACGAACTcgc > 5:108890/1‑149 (MQ=255) gCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCGATGATGCGCGTGGCCAGCGACGACACGAACTcgc > 2:87883/1‑149 (MQ=255) ctggCCATCTCGGATGGCCGGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCGATGATGCGCGTGGCCAGCGACGacac > 8:115424/3‑139 (MQ=255) ctggCCATCTCGGATGGCCGGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCGATGATGCGCGTGGCCAGCGACGacac < 7:115424/137‑1 (MQ=255) ccGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCGATGATGCGCGTGGCCAGCGACGACACg > 8:139964/1‑140 (MQ=255) ccGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCGATGATGCGCGTGGCCAGCGACGACACg < 7:139964/140‑1 (MQ=255) cATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCGATGATGCGCGTGGCCAGCGACGACACGAACTCGCGGTCGTg < 2:232116/149‑1 (MQ=255) aTCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCGATGATGCGCGTGGCCAGCGACGACACGAACTCGCGGTCGTgg < 6:108890/149‑1 (MQ=255) tCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTc < 4:100667/81‑1 (MQ=255) tCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTc > 3:100667/1‑81 (MQ=255) tCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCGATGATGCGCGTGGCCAGCGACGACACGAACTCGCGGTCGTGGc > 2:257899/1‑149 (MQ=255) cGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCGATGATGCGCGTGGCCAGCGACGACACGAACTCGCGGTCGTGGCt > 8:35032/1‑148 (MQ=255) cGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCGATGATGCGCGTGGCCAGCGACGACACGAACTCGCGGTCGTGGCTg < 4:9755/149‑1 (MQ=255) gagaTGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCGATGATGCGCGTGGCCAGCGACGACACGAACTCGCGGTCGTGGCTGa > 5:27023/1‑149 (MQ=255) gagaTGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCGATGATGCGCGTGGCCAGCGACGACACGAACTCGCGGTCGTGGCTGa < 1:148666/149‑1 (MQ=255) agaTGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCg > 3:55777/1‑102 (MQ=255) agaTGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCg < 4:55777/102‑1 (MQ=255) | CGAATTATTCGTCGGGCCGTGCCGGTCGGTGGGATGGGGATGTACTCTTCGCGGGCTTGCCCGCTCTCCCAAGGCCCCACAAGCGTCCAATACCTGTGCGGTCCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCCGGCCATCCGAGATGGCCAGGCC‑‑GT‑CCCATCAATCAGACCAGCACACCCTGGCTACGCAGGTAGTCATCGTAGGTACCGCTGAAGTCCACCACACCATCGGCCGACAGCTCGATGATGCGCGTGGCCAGCGACGACACGAACTCGCGGTCGTGGCTGA > NC_002947/3339073‑3339365 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |