| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 213,354 | T→A | 10.3% | L6287Q (CTG→CAG) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 213,354 | 0 | T | A | 10.3% | 160.9 / 8.3 | 58 | L6287Q (CTG→CAG) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (25/27); new base A (3/3); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01 | |||||||||||
GTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGCCAC > NC_002947/213206‑213498 | gTCTGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCt < 6:70185/149‑1 (MQ=255) gCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTc < 4:277171/149‑1 (MQ=255) ggTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGAcc < 8:1522/148‑1 (MQ=255) gacgaGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGcaa > 1:71156/1‑149 (MQ=255) ccGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGca < 7:4153/142‑1 (MQ=255) ccGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGca > 8:4153/1‑142 (MQ=255) gggTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAg > 6:15655/1‑138 (MQ=255) gggTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAg < 5:15655/138‑1 (MQ=255) ggTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGcc > 3:97488/1‑147 (MQ=255) cccAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtca > 8:197318/1‑149 (MQ=255) cccAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtc > 2:337263/1‑148 (MQ=255) cccAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCAGGTCGTGag > 8:188467/1‑125 (MQ=255) cccAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCAGGTCGTGag < 7:188467/126‑2 (MQ=255) cccAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCAGGTCGTGa < 7:79232/125‑1 (MQ=255) cccAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCAGGTCGTGa > 8:79232/1‑125 (MQ=255) ccAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcat > 1:245428/1‑149 (MQ=255) cAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcatc < 8:181493/149‑1 (MQ=255) cAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcatc < 4:120412/149‑1 (MQ=255) cAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcat > 3:120412/1‑148 (MQ=255) cAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcat > 8:77150/1‑148 (MQ=255) aaCGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcatcatc > 3:299891/1‑147 (MQ=255) cGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGG‑TCACGACCAGGTCGTGAgccaa < 7:290239/120‑3 (MQ=17) cGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGG‑TCACGACCAGGTCGTGAgccaa > 8:290239/1‑118 (MQ=17) ggCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTgg < 8:271042/149‑1 (MQ=255) ggCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTgg < 2:245428/149‑1 (MQ=255) gTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAGCACAGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAg > 4:152194/1‑149 (MQ=255) ggTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAgcgcgc > 3:234739/1‑149 (MQ=255) tCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCa < 4:299891/149‑1 (MQ=255) aCCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATa < 7:77150/149‑1 (MQ=255) cATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCGTaca > 6:183214/1‑149 (MQ=255) cGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCAc < 8:164376/149‑1 (MQ=255) ccGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCt < 4:234739/149‑1 (MQ=255) gACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTgc < 3:231890/149‑1 (MQ=255) aCCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTgcg > 4:107536/1‑149 (MQ=255) ccAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTgcg > 6:96841/1‑148 (MQ=255) ggTGGCCTAGAACGTCAAGCCGACGTTCACCTTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGa < 2:41297/147‑1 (MQ=255) ggTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAt > 8:56183/1‑148 (MQ=255) ccGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTc < 5:96841/149‑1 (MQ=255) cGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAt < 5:254951/142‑1 (MQ=255) cGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAt > 6:254951/1‑142 (MQ=255) gagaACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTa > 1:302285/1‑149 (MQ=255) aCGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAAc < 2:71156/149‑1 (MQ=255) gCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTg < 1:49715/149‑1 (MQ=255) cGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGc < 3:107536/149‑1 (MQ=255) cacaTCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTggg < 1:337263/149‑1 (MQ=255) aTCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTg > 2:217375/1‑70 (MQ=255) aTCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAAAGCCCAGGTCATCATCAAGGCTGGCGAAACCAGAGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCa > 7:232638/1‑148 (MQ=255) tCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCAtt < 7:56183/149‑1 (MQ=255) acCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTc < 4:97488/149‑1 (MQ=255) ccGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCg < 3:152194/149‑1 (MQ=255) ccTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCTTACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCa < 5:183214/149‑1 (MQ=255) cTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGc < 1:270850/130‑1 (MQ=255) cTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGc > 2:270850/1‑130 (MQ=255) cTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAg < 5:147105/149‑1 (MQ=255) tCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTg > 7:30082/1‑145 (MQ=255) tCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTg < 8:30082/145‑1 (MQ=255) aCGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGTCGAAACCAGAGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGc > 4:266043/1‑148 (MQ=255) aCGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGcc > 2:133349/1‑149 (MQ=255) gACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGCCAc > 2:17769/1‑149 (MQ=255) | GTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGCCAC > NC_002947/213206‑213498 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |