Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,475,489 G→C 11.2% H408Q (CAC→CAG) ‡ PP_3087 ← excinuclease ABC subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,475,4890GC11.2% 90.7 / 2.7 34H408Q (CAC→CAG) ‡PP_3087excinuclease ABC subunit A
Reads supporting (aligned to +/- strand):  ref base G (23/7);  new base C (2/2);  total (25/9)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

GCCGTGTTCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAGCCGAGGCCGA  >  NC_002947/3475345‑3475631
                                                                                                                                                  |                                                                                                                                              
gccgTGTTCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGc                                                                                                                                             >  4:84486/1‑148 (MQ=255)
gccgTGTTCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGc                                                                                                                                             >  7:173681/1‑148 (MQ=255)
          ccGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGCACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGa                                                                                                                                  <  6:348857/149‑1 (MQ=255)
           cGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGAc                                                                                                                                 <  3:84486/149‑1 (MQ=255)
                    gCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAgc                                                                                                                                         >  8:75284/1‑132 (MQ=255)
                    gCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAgc                                                                                                                                         <  7:75284/132‑1 (MQ=255)
                     cccTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCtcgtg                                                                                                                         >  4:332658/1‑146 (MQ=255)
                         aCATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTAc                                                                                                                   >  7:133563/1‑149 (MQ=255)
                               accaGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTTGAGGCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTa                                                                                                                >  1:283689/1‑148 (MQ=255)
                               accaGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTACAAGCCGGCCGACGGCTCGTCGAGTACCTAAac                                                                                                             >  4:86921/1‑149 (MQ=255)
                               accaGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGa                                                                                                                                  <  3:342199/128‑1 (MQ=255)
                               accaGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGa                                                                                                                                  >  4:342199/1‑128 (MQ=255)
                                               ggcgcAGGGCGTCCCGCTCATGCTCCACCACGAACCCCGAGTT‑‑CCCCCCCTTTTCCAGGCGCTGCTGGGCTTCGAACCGCCCTGCCCTGTCGGCCGGGTGCAACCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGc                                                                                             <  4:283915/149‑1 (MQ=255)
                                                        tgtCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCAccc                                                                                                        >  7:218963/1‑129 (MQ=255)
                                                            cAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTggg                                                                                >  3:96270/1‑149 (MQ=255)
                                                            cAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTggg                                                                                >  8:115907/1‑149 (MQ=255)
                                                             aGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGt                                                                               >  5:15981/1‑149 (MQ=255)
                                                                 cATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGAca                                                                                                                                <  6:277341/96‑2 (MQ=38)
                                                                 cATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGAca                                                                                                                                >  5:277341/1‑95 (MQ=38)
                                                                    gCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTg                                                                                                                                              >  5:261796/1‑79 (MQ=255)
                                                                    gCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTg                                                                                                                                              <  6:261796/79‑1 (MQ=255)
                                                                       ccaccaCGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCcaggcg                                                                      >  1:302882/1‑148 (MQ=255)
                                                                         accacGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCcaggcgcag                                                                   >  2:253572/1‑149 (MQ=255)
                                                                            acGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCcaggcgcaggcg                                                                >  5:185000/1‑149 (MQ=255)
                                                                                    cGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGtt                                                        >  2:340580/1‑149 (MQ=255)
                                                                                     gAGTT‑‑GCCAGCCTGATTCAGGCGCTGCAGGGCTTCGAACCGCGCTTCACTGTCGGCCTGGTGCAAGCCGGCCGACGGCTCGTCGAGGACGTAACCCCCAACAACACGCTGCGAATTCAGATGGGTGGCCAGGCGCAGGCGTTGAAGtt                                                        >  2:117040/1‑148 (MQ=255)
                                                                                             ccAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCag                                                  >  2:14097/1‑148 (MQ=255)
                                                                                               aGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCag                                                  >  5:323803/1‑146 (MQ=255)
                                                                                                   tGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGAc                                             >  5:170718/1‑147 (MQ=255)
                                                                                                        cAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCg                                      >  4:191953/1‑149 (MQ=255)
                                                                                                         aGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCgg                                     >  8:148519/1‑149 (MQ=255)
                                                                                                             gcggccgggCTTCGAACAGCGCTTCCCTGTCGGCCGGGTGCACGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGtg                                 <  6:170718/143‑1 (MQ=255)
                                                                                                                          gAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGtgct                               >  5:39950/1‑138 (MQ=255)
                                                                                                                                            ggCGGGGTCCAAGCCGCCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAgccgaggccga  <  4:96270/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
GCCGTGTTCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAGCCGAGGCCGA  >  NC_002947/3475345‑3475631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑