Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,475,491 G→T 14.7% H408N (CAC→AAC) ‡ PP_3087 ← excinuclease ABC subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,475,4910GT14.7% 77.4 / 5.4 32H408N (CAC→AAC) ‡PP_3087excinuclease ABC subunit A
Reads supporting (aligned to +/- strand):  ref base G (21/5);  new base T (2/2);  total (24/8)
Fisher's exact test for biased strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GCCGTGTTCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAGCCGAGGCCGAGGTCAAG  >  NC_002947/3475345‑3475638
                                                                                                                                                    |                                                                                                                                                   
gccgTGTTCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGc                                                                                                                                                    >  4:84486/1‑148 (MQ=255)
gccgTGTTCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGc                                                                                                                                                    >  7:173681/1‑148 (MQ=255)
          ccGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGCACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGa                                                                                                                                         <  6:348857/149‑1 (MQ=255)
           cGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGAc                                                                                                                                        <  3:84486/149‑1 (MQ=255)
                    gCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAgc                                                                                                                                                >  8:75284/1‑132 (MQ=255)
                    gCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAgc                                                                                                                                                <  7:75284/132‑1 (MQ=255)
                     cccTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCtcgtg                                                                                                                                >  4:332658/1‑146 (MQ=255)
                         aCATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTAc                                                                                                                          >  7:133563/1‑149 (MQ=255)
                               accaGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTTGAGGCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTa                                                                                                                       >  1:283689/1‑148 (MQ=255)
                               accaGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTACAAGCCGGCCGACGGCTCGTCGAGTACCTAAac                                                                                                                    >  4:86921/1‑149 (MQ=255)
                               accaGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGa                                                                                                                                         <  3:342199/128‑1 (MQ=255)
                               accaGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGa                                                                                                                                         >  4:342199/1‑128 (MQ=255)
                                               ggcgcAGGGCGTCCCGCTCATGCTCCACCACGAACCCCGAGTT‑‑CCCCCCCTTTTCCAGGCGCTGCTGGGCTTCGAACCGCCCTGCCCTGTCGGCCGGGTGCAACCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGc                                                                                                    <  4:283915/149‑1 (MQ=255)
                                                        tgtCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCAccc                                                                                                               >  7:218963/1‑129 (MQ=255)
                                                            cAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTggg                                                                                       >  3:96270/1‑149 (MQ=255)
                                                            cAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTggg                                                                                       >  8:115907/1‑149 (MQ=255)
                                                             aGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGt                                                                                      >  5:15981/1‑149 (MQ=255)
                                                                 cATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGAca                                                                                                                                       <  6:277341/96‑2 (MQ=38)
                                                                 cATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGAca                                                                                                                                       >  5:277341/1‑95 (MQ=38)
                                                                    gCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTg                                                                                                                                                     >  5:261796/1‑79 (MQ=255)
                                                                    gCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTg                                                                                                                                                     <  6:261796/79‑1 (MQ=255)
                                                                       ccaccaCGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCcaggcg                                                                             >  1:302882/1‑148 (MQ=255)
                                                                         accacGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCcaggcgcag                                                                          >  2:253572/1‑149 (MQ=255)
                                                                            acGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCcaggcgcaggcg                                                                       >  5:185000/1‑149 (MQ=255)
                                                                                    cGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGtt                                                               >  2:340580/1‑149 (MQ=255)
                                                                                     gAGTT‑‑GCCAGCCTGATTCAGGCGCTGCAGGGCTTCGAACCGCGCTTCACTGTCGGCCTGGTGCAAGCCGGCCGACGGCTCGTCGAGGACGTAACCCCCAACAACACGCTGCGAATTCAGATGGGTGGCCAGGCGCAGGCGTTGAAGtt                                                               >  2:117040/1‑148 (MQ=255)
                                                                                             ccAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCag                                                         >  2:14097/1‑148 (MQ=255)
                                                                                               aGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCag                                                         >  5:323803/1‑146 (MQ=255)
                                                                                                   tGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGAc                                                    >  5:170718/1‑147 (MQ=255)
                                                                                                        cAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCg                                             >  4:191953/1‑149 (MQ=255)
                                                                                                         aGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCgg                                            >  8:148519/1‑149 (MQ=255)
                                                                                                             gcggccgggCTTCGAACAGCGCTTCCCTGTCGGCCGGGTGCACGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGtg                                        <  6:170718/143‑1 (MQ=255)
                                                                                                                          gAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGtgct                                      >  5:39950/1‑138 (MQ=255)
                                                                                                                                            ggCGGGGTCCAAGCCGCCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAgccgaggccga         <  4:96270/149‑1 (MQ=255)
                                                                                                                                                    gCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAGCCGAGGCCGAGGTCAag  >  5:317547/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCGTGTTCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAGCCGAGGCCGAGGTCAAG  >  NC_002947/3475345‑3475638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑