| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,475,492 | C→G | 13.0% | L407F (TTG→TTC) ‡ | PP_3087 ← | excinuclease ABC subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,475,492 | 0 | C | G | 13.0% | 68.9 / 5.4 | 31 | L407F (TTG→TTC) ‡ | PP_3087 | excinuclease ABC subunit A |
| Reads supporting (aligned to +/- strand): ref base C (21/6); new base G (2/2); total (23/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.52e-01 | |||||||||||
GCCGTGTTCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAGCCGAGGCCGAGGTCAAG > NC_002947/3475345‑3475638 | gccgTGTTCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGc > 4:84486/1‑148 (MQ=255)gccgTGTTCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGc > 7:173681/1‑148 (MQ=255) ccGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGCACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGa < 6:348857/149‑1 (MQ=255) cGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGAc < 3:84486/149‑1 (MQ=255) gCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAgc > 8:75284/1‑132 (MQ=255) gCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAgc < 7:75284/132‑1 (MQ=255) cccTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCtcgtg > 4:332658/1‑146 (MQ=255) aCATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTAc > 7:133563/1‑149 (MQ=255) accaGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTTGAGGCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTa > 1:283689/1‑148 (MQ=255) accaGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTACAAGCCGGCCGACGGCTCGTCGAGTACCTAAac > 4:86921/1‑149 (MQ=255) accaGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGa < 3:342199/128‑1 (MQ=255) accaGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGa > 4:342199/1‑128 (MQ=255) ggcgcAGGGCGTCCCGCTCATGCTCCACCACGAACCCCGAGTT‑‑CCCCCCCTTTTCCAGGCGCTGCTGGGCTTCGAACCGCCCTGCCCTGTCGGCCGGGTGCAACCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGc < 4:283915/149‑1 (MQ=255) tgtCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCAccc > 7:218963/1‑129 (MQ=255) cAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTggg > 3:96270/1‑149 (MQ=255) cAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTggg > 8:115907/1‑149 (MQ=255) aGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGt > 5:15981/1‑149 (MQ=255) cATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGAca > 5:277341/1‑95 (MQ=38) cATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGAca < 6:277341/96‑2 (MQ=38) ccaccaCGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCcaggcg > 1:302882/1‑148 (MQ=255) accacGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCcaggcgcag > 2:253572/1‑149 (MQ=255) acGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCcaggcgcaggcg > 5:185000/1‑149 (MQ=255) cGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGtt > 2:340580/1‑149 (MQ=255) gAGTT‑‑GCCAGCCTGATTCAGGCGCTGCAGGGCTTCGAACCGCGCTTCACTGTCGGCCTGGTGCAAGCCGGCCGACGGCTCGTCGAGGACGTAACCCCCAACAACACGCTGCGAATTCAGATGGGTGGCCAGGCGCAGGCGTTGAAGtt > 2:117040/1‑148 (MQ=255) ccAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCag > 2:14097/1‑148 (MQ=255) aGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCag > 5:323803/1‑146 (MQ=255) tGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGAc > 5:170718/1‑147 (MQ=255) cAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCg > 4:191953/1‑149 (MQ=255) aGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCgg > 8:148519/1‑149 (MQ=255) gcggccgggCTTCGAACAGCGCTTCCCTGTCGGCCGGGTGCACGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGtg < 6:170718/143‑1 (MQ=255) gAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGtgct > 5:39950/1‑138 (MQ=255) ggCGGGGTCCAAGCCGCCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAgccgaggccga < 4:96270/149‑1 (MQ=255) gCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAGCCGAGGCCGAGGTCAag > 5:317547/1‑148 (MQ=255) | GCCGTGTTCGCCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAGCCGAGGCCGAGGTCAAG > NC_002947/3475345‑3475638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |