| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,475,495 | G→C | 17.0% | G406G (GGC→GGG) | PP_3087 ← | excinuclease ABC subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,475,495 | 0 | G | C | 17.0% | 60.9 / 4.9 | 29 | G406G (GGC→GGG) | PP_3087 | excinuclease ABC subunit A |
| Reads supporting (aligned to +/- strand): ref base G (20/4); new base C (2/3); total (22/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.47e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAGCCGAGGCCGAGGTCAAGCAGCG > NC_002947/3475355‑3475643 | ccGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGCACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGa < 6:348857/149‑1 (MQ=255) cGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGAc < 3:84486/149‑1 (MQ=255) gCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAgc > 8:75284/1‑132 (MQ=255) gCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAgc < 7:75284/132‑1 (MQ=255) cccTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCtcgtg > 4:332658/1‑146 (MQ=255) aCATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTAc > 7:133563/1‑149 (MQ=255) accaGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTTGAGGCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTa > 1:283689/1‑148 (MQ=255) accaGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTACAAGCCGGCCGACGGCTCGTCGAGTACCTAAac > 4:86921/1‑149 (MQ=255) accaGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGa < 3:342199/128‑1 (MQ=255) accaGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGa > 4:342199/1‑128 (MQ=255) ggcgcAGGGCGTCCCGCTCATGCTCCACCACGAACCCCGAGTT‑‑CCCCCCCTTTTCCAGGCGCTGCTGGGCTTCGAACCGCCCTGCCCTGTCGGCCGGGTGCAACCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGc < 4:283915/149‑1 (MQ=255) tgtCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCAccc > 7:218963/1‑129 (MQ=255) cAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTggg > 3:96270/1‑149 (MQ=255) cAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTggg > 8:115907/1‑149 (MQ=255) aGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGt > 5:15981/1‑149 (MQ=255) cATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGAca > 5:277341/1‑95 (MQ=38) cATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGCTTGCACCCGGCCGAca < 6:277341/96‑2 (MQ=38) ccaccaCGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCcaggcg > 1:302882/1‑148 (MQ=255) accacGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCcaggcgcag > 2:253572/1‑149 (MQ=255) acGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCcaggcgcaggcg > 5:185000/1‑149 (MQ=255) cGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGtt > 2:340580/1‑149 (MQ=255) gAGTT‑‑GCCAGCCTGATTCAGGCGCTGCAGGGCTTCGAACCGCGCTTCACTGTCGGCCTGGTGCAAGCCGGCCGACGGCTCGTCGAGGACGTAACCCCCAACAACACGCTGCGAATTCAGATGGGTGGCCAGGCGCAGGCGTTGAAGtt > 2:117040/1‑148 (MQ=255) ccAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCag > 2:14097/1‑148 (MQ=255) aGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCag > 5:323803/1‑146 (MQ=255) tGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGAc > 5:170718/1‑147 (MQ=255) cAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCg > 4:191953/1‑149 (MQ=255) aGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCgg > 8:148519/1‑149 (MQ=255) gcggccgggCTTCGAACAGCGCTTCCCTGTCGGCCGGGTGCACGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGtg < 6:170718/143‑1 (MQ=255) gAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGtgct > 5:39950/1‑138 (MQ=255) ggCGGGGTCCAAGCCGCCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAgccgaggccga < 4:96270/149‑1 (MQ=255) gCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAGCCGAGGCCGAGGTCAag > 5:317547/1‑148 (MQ=255) gccggccgACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAGCCGAGGCCGAGGTCAAGCAGCg < 1:14097/149‑1 (MQ=255) | CCGGCAGCGGGCCCTACATCCACCAGCCAGTCGGCGCGGCGCATGGTGTCCAGGTCATGCTCCACCACGTACACCGAGTT‑‑GCCAGCCTGTTTCAGGCGCTGCAGGGCTTCGAACAGCGCTTCACTGTCGGCCGGGTGCAAGCCGGCCGACGGCTCGTCGAGTACGTAAACCACCCCAAACAGCTGCGAATTCAGCTGGGTGGCCAGGCGCAGGCGTTGCAGTTCACCAGAAGACAAGGTCGGGGTGCTGCGCTCCAGGGCCAGGTAGCCGAGGCCGAGGTCAAGCAGCG > NC_002947/3475355‑3475643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |