Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	236,407	C→T	7.8%	R143H (CGC→CAC)	argH ←	argininosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	236,407	0	C	T	7.8%	144.2 / 2.1	51	R143H (CGC→CAC)	argH	argininosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base C (24/23);  new base T (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

CAGGCGCTCATAGTCGCGGCTGAGCATTTCGAACCACGCCAGCAGGTGGTGGCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCAGTATGCAGCTTCTTGCCGGTGATGCCGATGCGGTCGGTCAGGCGTGCTTCGATGTTCAT  >  NC_002947/236259‑236552                                                                                                                                                     |                                                                                                                                                  cAGGCGCTCATAGTCGCGGCTGAGCATTTCGAACCACGCCAGCAGGTGGTGGCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGc                                                                                                                                                   >  7:44441/1‑149 (MQ=255)          aTAGTCGCGGCTGAGCATTTCGAACCACGCCAGCAGGTGGTGGCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGAttt                                                                                                                                          >  4:150766/1‑149 (MQ=255)              tCGCGGCTGAGCATTTCGAACCACGCCAGCAGGTGGTGGCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGcc                                                                                                                                      >  6:206675/1‑149 (MQ=255)                     tGAGCATTTCGAACCACGCCAGCAGGTGGTGGCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGAt                                                                                                                                >  4:328226/1‑148 (MQ=255)                        gCATTTCGAACCACGCCAGCAGGTGGTGGCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAgg                                                                                                                            >  1:310794/1‑149 (MQ=255)                            ttCGAACCACGCCAGCAGGTGGTGGCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCg                                                                                                                         >  5:293454/1‑148 (MQ=255)                            ttCGAACCACGCCAGCAGGTGGTGGCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCg                                                                                                                         <  6:293454/148‑1 (MQ=255)                              cGAACCACGCCAGCAGGTGGTGGCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGAtt                                                                                                                      >  3:250271/1‑149 (MQ=255)                                   cACGCCAGCAGGTGGTGGCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTtcgtc                                                                                                                 <  7:129351/149‑1 (MQ=255)                                    aCGCCAACAGGTGGTGGCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGAtt                                                                                                                                           >  4:31602/1‑122 (MQ=255)                                    aCGCCAACAGGTGGTGGCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGAtt                                                                                                                                           <  3:31602/122‑1 (MQ=255)                                     cGCCAGCAGGTGGTGGCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTcgc                                                                                                               <  4:250271/149‑1 (MQ=255)                                      gCCAGCAGGTGGTGGCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAg                                                                                                                                   >  7:62779/1‑128 (MQ=255)                                                 gtggCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGAttt                                                                                                                     >  5:238223/1‑131 (MQ=255)                                                 gtggCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGAttt                                                                                                                     <  6:238223/131‑1 (MQ=255)                                                 gtggCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGc                                                                                                   >  3:292052/1‑149 (MQ=255)                                                 gtggCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGc                                                                                                   <  5:206675/149‑1 (MQ=255)                                                    gCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGa                                                                                                <  3:328226/149‑1 (MQ=255)                                                     ccAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGAt                                                                                               <  2:310794/149‑1 (MQ=255)                                                      cAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGGATGATGGTTTCGGCTTCACGCACTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATg                                                                                              >  6:75453/1‑149 (MQ=255)                                                      cAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATg                                                                                              >  1:245965/1‑149 (MQ=255)                                                               ccGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGcc                                                                                     <  3:208258/149‑1 (MQ=255)                                                                cGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCa                                                                                    >  2:232367/1‑149 (MQ=255)                                                                 ggCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCAc                                                                                   <  1:232367/149‑1 (MQ=255)                                                                                  gtgggtgAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTAc                                                                  >  4:130200/1‑149 (MQ=255)                                                                                     ggtgAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGcc                                                               <  2:245965/149‑1 (MQ=255)                                                                                      gtgAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGc                                                                                      >  6:144634/1‑125 (MQ=255)                                                                                       tgAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGTGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGcc                                                               >  6:179396/1‑147 (MQ=255)                                                                                       tgAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGTGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGcc                                                               >  4:342860/1‑147 (MQ=255)                                                                                       tgAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGTGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCa                                                             <  5:179396/149‑1 (MQ=255)                                                                                       tgAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGTGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCa                                                             <  3:342860/149‑1 (MQ=255)                                                                                          aaCCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGtt                                                                          >  6:351166/1‑133 (MQ=255)                                                                                          aaCCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGtt                                                                          <  5:351166/133‑1 (MQ=255)                                                                                             cAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCAGTATGc                                                       <  8:25202/149‑1 (MQ=255)                                                                                                  atgatgGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCAGTATGCAGctt                                                  >  8:217384/1‑149 (MQ=255)                                                                                                           tCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCAGTATGCAGCTTCTTGCCgg                                          <  4:292052/148‑1 (MQ=255)                                                                                                           tCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCAGTATGCAGCTTCTTGCCgg                                          <  8:44441/148‑1 (MQ=255)                                                                                                                ttCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCAGTATGCAGCTTCTTGCCGGTGATGc                                    >  8:255376/1‑149 (MQ=255)                                                                                                                ttCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCAGTATGCAGCTTCTTGCCGGTGATGc                                    >  5:272070/1‑149 (MQ=255)                                                                                                                    cGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCAGTATGCAGCTTCTTGCCGGTGATGCCGAt                                <  3:150766/149‑1 (MQ=255)                                                                                                                       tctGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCAGTATGCAGCTTCTTGCCGGTGATGCCGATGc                              >  6:76531/1‑148 (MQ=255)                                                                                                                         tgcctgcTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCAGTATGCAGCTTCTTGCCGGTGATGCCGATGc                              <  5:76531/146‑1 (MQ=255)                                                                                                                           cctgcTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCgg                                                                      >  6:43878/1‑104 (MQ=255)                                                                                                                           cctgcTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCAGTATGCAGCTTCTTGCCGGTGATGCCGATGcggtcg                         >  5:284275/1‑149 (MQ=255)                                                                                                                           cctgcTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCAGTATGCAGCTTCTTGCCGGTGATGCCGATGcggtcg                         >  3:83934/1‑149 (MQ=255)                                                                                                                           cctgcTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGGCGTTGCGGCTACGCCCAGTATGCAGCTTCTTGCCGGTGATGCCGATGcgg                            <  5:75453/146‑1 (MQ=255)                                                                                                                             tgcTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCAGTATGCAGCTTCTTGCCGGTGATGCCGATGcggtcggt                       >  3:79614/1‑149 (MQ=255)                                                                                                                                        gCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCAGTATGCAGCTTCTTGCCGGTGATGCCGATGCGGTCGGTCAGGCGTGCtt            <  6:272070/149‑1 (MQ=255)                                                                                                                                         cccTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCAGTATGCAGCTTCTTGCCGGTGATGCCGATGCGGTCGGTCAGGCGTGCTTc           <  3:130200/149‑1 (MQ=255)                                                                                                                                              ctgcAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCAGTATGCAGCTTCTTGCCGGTGATGCCGATGCGGTCGGTCAGGCGTGCTTCGAt        <  6:98915/147‑1 (MQ=255)                                                                                                                                                  aGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCAGTATGCAGCTTCTTGCCGGTGATGCCGATGCGGTCGGTCAGGCGTGCTTCGATGTTCAt  <  7:255376/149‑1 (MQ=255)                                                                                                                                                  aGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCAGTATGCAGCTTCTTGCCGGTGATGCCGATGCGGTCGGTCAGGCGTGCTTCGATGTTCAt  <  8:72181/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CAGGCGCTCATAGTCGCGGCTGAGCATTTCGAACCACGCCAGCAGGTGGTGGCCAAAGGTGACCGGCTGCGCCGTCTGCAGGTGGGTGAAACCAGGCATGATGGTTTCGGCTTCACGCTCTGCCTGCTCCAGCAGGCCCTGCTGCAGGCGGGTGATTTCGCCCAGGATCAGGTCGATTTCGTCGCGCAGCCAAAGGCGGATGTCGGTGGCCACCTGGTCGTTGCGGCTACGCCCAGTATGCAGCTTCTTGCCGGTGATGCCGATGCGGTCGGTCAGGCGTGCTTCGATGTTCAT  >  NC_002947/236259‑236552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑