Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,529,780 T→C 10.4% L533L (TTG→CTG)  dnaEB → error‑prone DNA polymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,529,7800TC10.4% 125.7 / 4.4 48L533L (TTG→CTG) dnaEBerror‑prone DNA polymerase
Reads supporting (aligned to +/- strand):  ref base T (21/22);  new base C (2/3);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AGCACCCGGGCGGTTTTGTCATCTCCCAGCAACCGCTGGACCAGCTGGTGCCGGTGGAGAATGCCGCGATGCCGGAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGACCCGGCCACCTACGCCATGATCAGCCGTGCCGAGACCATGGGCGTGTTCCAG  >  NC_002947/3529634‑3529923
                                                                                                                                                  |                                                                                                                                               
cgCACCCGGGCGGTTTTGTCATCTCCCAGCAACCGCTGGACCAGCTGGTGCCGGTGGAGAATGCCGCGATGCCGGAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTg                                                                                                                                               <  8:138101/148‑1 (MQ=255)
      cGGGCGGTTTTGTCATCTCCCAGCAACCGCTGGACCAGCTGGTGCCGGTGGAGAATGCCGCGATGCCGGAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATg                                                                                                                                         >  8:325882/1‑149 (MQ=255)
              tttGTCATCTCCCAGCAACCGCTGGACCAGCTGGTGCCGGTGGAGAATGCCGCGATGCCGGAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAgcgc                                                                                                                                 >  1:342670/1‑149 (MQ=255)
               ttGTCATCTCCCAGCAACCGCTGGACCAGCTGGTGCCGGTGGAGAATGCCGCGATGCCGGAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGCTGAGCATGCCCAacgc                                                                                                                                 <  2:85860/148‑1 (MQ=255)
                       tcCCAGCAACCGCTGGACCAGCTGGTGCCGGTGGAGAATGCCGCGATGCCGGAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCg                                                                                                                        >  7:198727/1‑149 (MQ=255)
                          cAGCAACCGCTGGACCAGCTGGTGCCGGTGGAGAATGCCGCGATGCCGGAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCgctg                                                                                                                     <  3:115378/149‑1 (MQ=255)
                           aGCAACCGCTGGACCAGCTGGTGCCGGTGGAGAATGCCGCGATGCCGGAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCgctg                                                                                                                     >  8:115045/1‑148 (MQ=255)
                                   cTGGACCAGCTGGTGCCGGTGGAGAATGCCGCGATGCCGGAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGAc                                                                                                              >  4:346817/1‑147 (MQ=255)
                                        ccAGCTGGTGCCGGTGGAGAATGCCGCGATGCCGGAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTgc                                                                                                       <  2:342670/149‑1 (MQ=255)
                                          aGCTGGTGCCGGTGGAGAATGCCGCGATGCCGGAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTgcag                                                                                                     <  7:115045/149‑1 (MQ=255)
                                          aGCTGGTGCCGGTGGAGAATGCCGCGATGCCGGAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGCTGAGCATGCCCAACGccag                                                                                                                              <  4:190779/124‑3 (MQ=255)
                                          aGCTGGTGCCGGTGGAGAATGCCGCGATGCCGGAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGCTGAGCATGCCCAACGccag                                                                                                                              >  3:190779/1‑122 (MQ=255)
                                           gCTGGTGCCGGTGGAGAATGCCGCGATGCCGGAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTgcagc                                                                                                    <  5:293589/149‑1 (MQ=255)
                                                  ccGGTGGTGAATGCCGCGATGCCGGAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCATGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCg                                                                                             <  2:321370/149‑1 (MQ=255)
                                                    ggTGGAGAATGCCGCGATGCCGGAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTg                                                                                           >  4:169971/1‑149 (MQ=255)
                                                          gaATGCCGCGATGCCGGAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGc                                                                                     <  8:198727/149‑1 (MQ=255)
                                                                  cgATGCCGGAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGAcc                                                                             <  3:169595/149‑1 (MQ=255)
                                                                         ggAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGa                                                                      <  1:319045/149‑1 (MQ=255)
                                                                           aGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGAcc                                                                    <  7:85868/149‑1 (MQ=255)
                                                                              gcACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCCCCGTGGCCGGCACCTGACCCTGGCGACCATc                                                                 >  4:308195/1‑149 (MQ=255)
                                                                              gcACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATc                                                                 >  5:200345/1‑149 (MQ=255)
                                                                                      aTCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGCTGAGCAt                                                                                                                                          >  4:231569/1‑68 (MQ=255)
                                                                                      aTCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGCTGAGCAt                                                                                                                                          <  3:231569/68‑1 (MQ=255)
                                                                                       tCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCg                                                          >  5:42038/1‑147 (MQ=255)
                                                                                       tCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGaa                                                        <  7:99708/149‑1 (MQ=255)
                                                                                         cAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGa                                                      >  1:187335/1‑149 (MQ=255)
                                                                                            tGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGAccc                                                   >  5:159935/1‑149 (MQ=255)
                                                                                            tGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGAccc                                                   <  6:200345/149‑1 (MQ=255)
                                                                                            tGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGGCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGAccc                                                   <  6:338829/149‑1 (MQ=255)
                                                                                               gACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGAACCGGc                                                >  3:132623/1‑149 (MQ=255)
                                                                                                         aCCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGACCCGGCCACCTACGc                                       <  7:325882/148‑1 (MQ=255)
                                                                                                           cTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGACCCGGCCACCTACGCCAt                                    >  8:42744/1‑149 (MQ=255)
                                                                                                           cTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGACCCGGCCACCTACGCCAt                                    <  2:187335/149‑1 (MQ=255)
                                                                                                           cTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGCCATGCTCAGCGCCTTGCGTCGCTCCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCAACCCCAGCGAACACCCGGCCACCAACCCCAt                                    >  4:90157/1‑149 (MQ=255)
                                                                                                               aCATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGACCCGGCCACCTACGcc                                      <  6:188901/143‑1 (MQ=255)
                                                                                                               aCATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGACCCGGCCACCTACGcc                                      >  5:188901/1‑143 (MQ=255)
                                                                                                                 aTGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGACCCGGCCACCTACGCCATGATCAg                              >  7:325192/1‑149 (MQ=255)
                                                                                                                   ggTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATc                                                                 >  4:193057/1‑112 (MQ=255)
                                                                                                                   ggTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATc                                                                 <  3:193057/112‑1 (MQ=255)
                                                                                                                   ggTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGACCCGGCCACCTACGCCATGATCAGcc                            >  4:146954/1‑149 (MQ=255)
                                                                                                                             cTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGACCCGGCCACCTACGCCATGATCAGCCGTGCCgaga                   <  6:42038/148‑1 (MQ=255)
                                                                                                                             cTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGACCCGGCCACCTACGCCATGATCAGCCGTGCCGAGAc                  <  6:159935/149‑1 (MQ=255)
                                                                                                                                  ggTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGACCCGGCCACCTACGCCATGATCAGCCGTGCCGAGACCATgg             <  3:346817/149‑1 (MQ=255)
                                                                                                                                          tACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGACCCGGCCACCTACGCCATGATCAGCCGTGCCGAGACCATGGGCGTGTTc     <  7:42744/149‑1 (MQ=255)
                                                                                                                                             tGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGACCCGGCCACCTACGCCATGATCAGCCGTGCCGAGACCATGGGCGTGTTc     >  5:343765/1‑146 (MQ=255)
                                                                                                                                               gCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGACCCGGCCACCTACGCCATGATCAGCCGTGCCGAGACCATGGGCGTGTTc     >  7:8883/1‑144 (MQ=255)
                                                                                                                                               gCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGACCCGGCCACCTACGCCATGATCAGCCGTGCCGAGACCATGGGCGTGTTc     >  3:64441/1‑144 (MQ=255)
                                                                                                                                               gCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGACCCGGCCACCTACGCCATGATCAGCCGTGCCGAGACCATGGGCGTGTTCCAg  <  6:343765/147‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
AGCACCCGGGCGGTTTTGTCATCTCCCAGCAACCGCTGGACCAGCTGGTGCCGGTGGAGAATGCCGCGATGCCGGAGCGCACGGTGATCCAGTGGGACAAGGATGACCTGGACATGGTTGGCCTGCTCAAGGTCGACGTACTGGCGTTGGGCATGCTCAGCGCCTTGCGTCGCTGCTTCGACCAGCTGCAGCACCACCGTGGCCGGCACCTGACCCTGGCGACCATCCCCAGCGAAGACCCGGCCACCTACGCCATGATCAGCCGTGCCGAGACCATGGGCGTGTTCCAG  >  NC_002947/3529634‑3529923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑