Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,545,276 C→A 8.3% M53I (ATG→ATT)  PP_3130 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,545,2760CA 8.3% 147.5 / 3.3 48M53I (ATG→ATT) PP_3130hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (23/21);  new base A (2/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

CCTCTTCCTTACGGATGTCAGCCTGCAGGTCGAAGTCCAGTTCCTTGTTCTTGCCCCGCAGCGTGTAGTGGTAAGGGCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCGGCAGCAGGGCCAGGCCGGCAATGGCCGAAAGGCGCACGTAATGACGCAGGGACAGCC  >  NC_002947/3545128‑3545421
                                                                                                                                                    |                                                                                                                                                 
ccTCTTCCTAACGGATGTCAGCCTGCAGGTCGAAGTCGCGTGCCTTGTTCTTGCCCCGCAGCGTGTAGTGGTAAGGGCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGGCCAGAAATCGATTTTGGCCTTc                                                                                                                                                   <  2:308205/149‑1 (MQ=255)
    ttCCTTACGGATGTCAGCCTGCAGGTCGAAGTCCAGTTCCTTGTTCTTGCCCCGCAGCGTGTAGTGGTAAGGGCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCAt                                                                                                                                                 <  1:154265/147‑1 (MQ=255)
                   aGCCTGCAGGTCGAAGTCCAGTTCCTTGTTCTTGCCCCGCAGCGTGTAGTGGTAAGGGCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCgt                                                                                                                                <  3:228735/149‑1 (MQ=255)
                    gCCTGCAGGTCGAAGTCCAGTTCCTTGTTCTTGCCCCGCAGCGTGTAGTGGTAAGGGCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCgtg                                                                                                                               <  3:325080/149‑1 (MQ=255)
                    gCCTGCAGGTCGAAGTCCAGTTCCTTGTTCTTGCCCCGCAGCGTGTAGTGGTAAGGGCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTAATGACCGGGc                                                                                                                                         >  5:241133/1‑139 (MQ=255)
                    gCCTGCAGGTCGAAGTCCAGTTCCTTGTTCTTGCCCCGCAGCGTGTAGTGGTAAGGGCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTAATGACCGGGc                                                                                                                                         >  5:236014/1‑139 (MQ=255)
                    gCCTGCAGGTCGAAGTCCAGTTCCTTGTTCTTGCCCCGCAGCGTGTAGTGGTAAGGGCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTAATGACCGGGc                                                                                                                                         <  6:236014/139‑1 (MQ=255)
                    gCCTGCAGGTCGAAGTCCAGTTCCTTGTTCTTGCCCCGCAGCGTGTAGTGGTAAGGGCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTAATGACCGGGc                                                                                                                                         <  6:241133/139‑1 (MQ=255)
                     ccTGCAGGTCGAAGTCCAGTTCCTTGTTCTTGCCCCGCAGCGTGTAGTGGTAAGGGCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCgtgt                                                                                                                              >  5:22222/1‑149 (MQ=255)
                                  gTCCAGTTCCTTGTTCTTGCCCCGCAGCGTGTAGTGGTAAGGGCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGc                                                                                                                  >  3:178781/1‑148 (MQ=255)
                                     cAGTTCCTTGTTCTTGCCCCGCAGCGTGTAGTGGTAAGGGCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGc                                                                                                              >  1:161548/1‑149 (MQ=255)
                                                      cccGCAGCGTGTAGTGGTAAGGGCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTAATAGac                                                                                              >  6:282175/1‑148 (MQ=255)
                                                             cGTGTAGTGGTAAGGGCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCt                                                                                      <  4:178781/149‑1 (MQ=255)
                                                                         cgggCCATGGACCTGCAAGTCGGTATAGACACCGGTCCAGCACCAGTTCGCCGCCCAGAAATCGAATTTGGCCTTCATGACCGCGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGaa                                                                           <  4:8701/147‑1 (MQ=255)
                                                                         aGGGCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCGGTCTGaa                                                                           <  6:22222/148‑1 (MQ=255)
                                                                          gggCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGc                                                                                                                  <  6:56900/108‑1 (MQ=255)
                                                                          gggCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGc                                                                                                                  >  5:56900/1‑108 (MQ=255)
                                                                            gCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCt                                                                              <  6:58328/142‑1 (MQ=255)
                                                                            gCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCt                                                                              >  5:58328/1‑142 (MQ=255)
                                                                             ccATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCgt                                                                                                                                >  3:39510/1‑91 (MQ=255)
                                                                             ccATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCgt                                                                                                                                <  4:39510/91‑1 (MQ=255)
                                                                                     cTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGc                                                                >  3:29007/1‑147 (MQ=255)
                                                                                      ttGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAg                                                             >  6:171435/1‑149 (MQ=255)
                                                                                        gAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAg                                                                          >  4:326010/1‑134 (MQ=255)
                                                                                        gAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAg                                                                          <  3:326010/134‑1 (MQ=255)
                                                                                            tCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCa                                                                      >  5:129035/1‑134 (MQ=255)
                                                                                            tCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCa                                                                      <  6:129035/134‑1 (MQ=255)
                                                                                              ggTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCgg                                                          <  7:132046/144‑1 (MQ=255)
                                                                                              ggTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCgg                                                          >  8:132046/1‑144 (MQ=255)
                                                                                                       aCCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGaca                                                                                             >  5:353046/1‑100 (MQ=255)
                                                                                                       aCCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGaca                                                                                             <  6:353046/100‑1 (MQ=255)
                                                                                                          ggTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCGGCAGCAGGGCCAGGCCgg                                         <  5:171435/149‑1 (MQ=255)
                                                                                                           gTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCGGCAGCAGGGCCAGGCCgg                                         >  5:58035/1‑148 (MQ=255)
                                                                                                             ccAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCGGCAGCAGGGCCAGGCCGGCaa                                      >  7:61716/1‑149 (MQ=255)
                                                                                                              cAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTgg                                                                 >  4:10724/1‑121 (MQ=255)
                                                                                                              cAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTgg                                                                 <  3:10724/121‑1 (MQ=255)
                                                                                                                  tCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCGGCAGCAGGGCCAGGCCGGCAATGGcc                                 >  2:160998/1‑149 (MQ=255)
                                                                                                                         ggCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCGGCAGCAGGGCCAGGCCGGCAATGGCCGAAAGgc                          >  5:262320/1‑149 (MQ=255)
                                                                                                                         ggCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCGGCAGCAGGGCCAGGCCGGCAATGGCCGAAAGgc                          >  1:361418/1‑149 (MQ=255)
                                                                                                                             gCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCGGCAGCAGGGCCAGGCCGGCAATGGCCGAAAGGCGCAc                      >  5:313904/1‑149 (MQ=255)
                                                                                                                             gCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCGGCAGCAGGGCCAGGCCGGCAATGGCCGAAAGGCGCAc                      >  6:185897/1‑149 (MQ=255)
                                                                                                                                    aaTCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCGGCAGCAGGGCCAGGCCGGCAATGGCCGAAAGGCGCACGTAATGa               <  4:29007/149‑1 (MQ=255)
                                                                                                                                        gAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCGGCAGCAGGGCCAGGCCGGCAATGGCCGAAAGGCGCACGTAATGACGc            <  5:185897/148‑1 (MQ=255)
                                                                                                                                          aTTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCGGCAGCAGGGCCAGGCCGGCAATGGCCGAAAGGCGCACGTAATGACGCAgg         <  6:262320/149‑1 (MQ=255)
                                                                                                                                                ccTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCGGCAGCAGGGCCAGGCCGGCAATGGCCGAAAGGCGCACGTAAt                 >  3:285703/1‑135 (MQ=255)
                                                                                                                                                ccTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCGGCAGCAGGGCCAGGCCGGCAATGGCCGAAAGGCGCACGTAAt                 <  4:285703/135‑1 (MQ=255)
                                                                                                                                                ccTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCGGCAGCAGGGCCAGGCCGGCAATGGCCGAAAGGCGCACGTAATGACGCAGGGACAGc   <  2:361418/149‑1 (MQ=255)
                                                                                                                                                ccTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCGGCAGCAGGGCCAGGCCGGCAATGGCCGAAAGGCGCACGTAATGACGCAGGGACAGc   <  2:35571/149‑1 (MQ=255)
                                                                                                                                                 cTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCGGCAGCAGGGCCAGGCCGGCAATGGCCGAAAGGCGCACGTAATGACGCAGGGACAGcc  >  2:225720/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CCTCTTCCTTACGGATGTCAGCCTGCAGGTCGAAGTCCAGTTCCTTGTTCTTGCCCCGCAGCGTGTAGTGGTAAGGGCCATTGACCTTGAAGTCGGTATAGAAACCGGTCCAGCTCCAGTTGGCAGCCCAGAAATCGAATTTGGCCTTCATGACCGGGCCACCGCCGTGTGTCACGTTAGGCAAGCCATTTTGCTCATCGACACTGACCTGCCAGTCTGAAGCCCAGCTGGCCAGCGGCAGCAGGGCCAGGCCGGCAATGGCCGAAAGGCGCACGTAATGACGCAGGGACAGCC  >  NC_002947/3545128‑3545421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑