Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 253,361 A→G 5.5% intergenic (‑146/+25) PP_0202 ← / ← PP_0203 CBS domain‑containing protein/dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947253,3610AG 5.5% 215.2 / 2.2 73intergenic (‑146/+25)PP_0202/PP_0203CBS domain‑containing protein/dipeptidase
Reads supporting (aligned to +/- strand):  ref base A (36/33);  new base G (2/2);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

ATACGGGCCTCTGCGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTCGTTGTCGTTGTGCCCGGTGTACTTCACCTCCGGCATG  >  NC_002947/253214‑253509
                                                                                                                                                    |                                                                                                                                                    
aTACGGGCCTCTGCGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAAc                                                                                                                                                     >  5:148378/1‑149 (MQ=255)
           tGCGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGTTTTAGCCGCGAaaag                                                                                                                                            <  8:245702/147‑1 (MQ=255)
             cGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCg                                                                                                                                        <  7:36052/149‑1 (MQ=255)
                 tGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTAc                                                                                                                                    >  5:106630/1‑149 (MQ=255)
                 tGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTAc                                                                                                                                    >  4:262943/1‑149 (MQ=255)
                    aaaaaGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTgc                                                                                                                                 <  1:163570/149‑1 (MQ=255)
                     aaaaGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTaaaa                                                                                                                                                      <  5:336152/127‑1 (MQ=255)
                     aaaaGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTaaaa                                                                                                                                                      >  6:336152/1‑127 (MQ=255)
                     aaaaGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATCCAGGAAACGGCATGGAGGCGATTGAAAAACGACATGGCTGTTCGCTTGTAGCGGCCTTATCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTgag                                                                                                                                >  5:363090/1‑147 (MQ=255)
                          cACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCa                                                                                                                           <  2:245528/149‑1 (MQ=255)
                            cTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCaaa                                                                                                                         <  6:106630/149‑1 (MQ=255)
                            cTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCaaa                                                                                                                         <  6:309527/149‑1 (MQ=255)
                               tctcGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGaa                                                                                                                                               <  4:231703/124‑1 (MQ=255)
                               tctcGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGaa                                                                                                                                               >  3:231703/1‑124 (MQ=255)
                                    gAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCgg                                                                                                                  >  4:220093/1‑148 (MQ=255)
                                     aGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCa                                                                                                                >  4:323763/1‑149 (MQ=255)
                                       tAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCtt                                                                                                                     >  1:344008/1‑142 (MQ=255)
                                       tAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCtt                                                                                                                     <  2:344008/142‑1 (MQ=255)
                                         aCTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTAATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAggg                                                                                                                                          >  7:226985/1‑118 (MQ=255)
                                          cTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGc                                                                                                           <  5:184832/149‑1 (MQ=255)
                                           tAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCg                                                                                                                                        >  6:32753/1‑119 (MQ=255)
                                           tAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCg                                                                                                                                        <  5:32753/119‑1 (MQ=255)
                                           tAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGcc                                                                                                          >  5:161007/1‑149 (MQ=255)
                                           tAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGcc                                                                                                          >  3:139805/1‑149 (MQ=255)
                                             aaGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGcc                                                                                                          <  2:9796/147‑1 (MQ=255)
                                               gCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACcgc                                                                                                                                                  >  4:87266/1‑105 (MQ=255)
                                               gCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACcgc                                                                                                                                                  <  3:87266/105‑1 (MQ=255)
                                               gCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGAt                                                                                                      >  3:254268/1‑149 (MQ=255)
                                                cATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATc                                                                                                     <  4:139805/149‑1 (MQ=255)
                                                cATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATc                                                                                                     >  5:349751/1‑149 (MQ=255)
                                                  tCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCcg                                                                                                   >  4:288083/1‑149 (MQ=255)
                                                    cGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCcgcg                                                                                                 >  8:259692/1‑149 (MQ=255)
                                                    cGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCcgc                                                                                                  <  3:262943/148‑1 (MQ=255)
                                                             gcTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTg                                                                                                                                  <  3:33667/107‑1 (MQ=255)
                                                             gcTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTg                                                                                                                                  >  4:33667/1‑107 (MQ=255)
                                                               tctGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCAt                                                                                                               >  7:151021/1‑124 (MQ=255)
                                                               tctGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCAt                                                                                                               <  8:151021/124‑1 (MQ=255)
                                                                  gCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCTCGAAGAGGACCGTACTGCGCATCAAAACTTCGGCATCTGCCCGATCCGAGCCACCATTACGCt                                                                                    >  1:176559/1‑148 (MQ=255)
                                                                  gCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCt                                                                                    >  6:103468/1‑148 (MQ=255)
                                                                  gCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTCTTCGC‑GGTTTTAGCCGCGAAAAGGCCGCTa                                                                                                                                     >  8:216119/1‑99 (MQ=25)
                                                                  gCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTCTTCGC‑GGTTTTAGCCGCGAAAAGGCCGCTa                                                                                                                                     <  7:216119/99‑1 (MQ=25)
                                                                   cACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCtaa                                                                                  >  6:251756/1‑147 (MQ=255)
                                                                       gCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTcaccac                                                                              <  4:254268/149‑1 (MQ=255)
                                                                       gCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTcaccac                                                                              >  6:126065/1‑149 (MQ=255)
                                                                         aaTGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCAGCCCCATCCGCGCCACCATTTCGCTCACCCCCt                                                                            >  4:298432/1‑149 (MQ=255)
                                                                           tGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGc                                                                          <  2:158025/149‑1 (MQ=255)
                                                                             cAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGc                                                                          >  2:173465/1‑147 (MQ=255)
                                                                             cAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCaa                                                                        <  3:323763/149‑1 (MQ=255)
                                                                                gAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTc                                                                     >  4:177929/1‑149 (MQ=255)
                                                                                 aaaCGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTcc                                                                    <  3:220093/149‑1 (MQ=255)
                                                                                  aaCGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCa                                                                   <  7:259692/149‑1 (MQ=255)
                                                                                    cGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGc                                                                 <  3:288083/149‑1 (MQ=255)
                                                                                           gAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACt                                                          >  1:360186/1‑149 (MQ=255)
                                                                                                  ttGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCACCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCAc                                                   >  6:223627/1‑149 (MQ=255)
                                                                                                    gAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACg                                                  <  1:36666/148‑1 (MQ=255)
                                                                                                       aaaCGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCt                                              <  1:173465/149‑1 (MQ=255)
                                                                                                        aaCGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCtt                                             <  6:161007/149‑1 (MQ=255)
                                                                                                           gTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACGGCGAAGAGGCCGGTACTGCGCATGAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGaa                                          <  2:360186/149‑1 (MQ=255)
                                                                                                            tCATGGCTGTTCGCTTGTAGCGGCCTTTTCGCTGGCTAAAACCGCGAAGAGGCCGGTAATGCGCATCAAAGCTTCGGCATCTGCCCGATCCTCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGaa                                          >  1:225519/1‑149 (MQ=255)
                                                                                                            tCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAAc                                         >  3:59333/1‑149 (MQ=255)
                                                                                                            tCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAAc                                         <  5:103468/149‑1 (MQ=255)
                                                                                                                 gCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACtcgtt                                    <  3:177929/149‑1 (MQ=255)
                                                                                                                    gTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTca                                                                                  >  5:335926/1‑100 (MQ=255)
                                                                                                                    gTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTca                                                                                  <  6:335926/100‑1 (MQ=255)
                                                                                                                     ttCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACtcgttgtcg                                <  4:59333/149‑1 (MQ=255)
                                                                                                                        gCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCCCCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACtcgttgtcgttg                             >  5:333649/1‑149 (MQ=255)
                                                                                                                          ttGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGGATGTACTGCTGCGCGGTGTTTAACTCGTTGTCGTtgtg                           >  2:136929/1‑149 (MQ=255)
                                                                                                                               gCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTCGTTGTCGTTGTGCCCg                       <  6:148378/148‑1 (MQ=255)
                                                                                                                               ccGGCCTCTTCGC‑GGTTTTAGCCGCGAAAAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTCGTTGTCGTTGTGCCCgg                      >  2:5213/2‑149 (MQ=39)
                                                                                                                                    cTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTCGTTGTCGTTGTGCCCGGTGTAc                 >  7:52980/1‑149 (MQ=255)
                                                                                                                                     ttttCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACtc                                       <  1:129944/126‑1 (MQ=255)
                                                                                                                                     ttttCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACtc                                       >  2:129944/1‑126 (MQ=255)
                                                                                                                                       ttCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTCGTTGTCGTTGTGCCCGGTGTACt                <  8:52980/147‑1 (MQ=255)
                                                                                                                                        tCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGGTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTCGTTGTCGTTGTGCCCGGTGTACTTc              <  6:363090/148‑1 (MQ=255)
                                                                                                                                          gc‑gGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTCGTTGTCGTTGTGCCCGGTGTACTTCAcc           >  3:66837/1‑149 (MQ=255)
                                                                                                                                                  aaaCCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACg                                                  <  3:190235/103‑1 (MQ=255)
                                                                                                                                                  aaaCCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACg                                                  >  4:190235/1‑103 (MQ=255)
                                                                                                                                                   aaCCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCTATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTCGTTGTCGTTGTGCCCGGTGTACTTCACCTCCGGCAt   <  3:298432/149‑1 (MQ=255)
                                                                                                                                                    aCCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTCGTTGTCGTTGTGCCCGGTGTACTTCACCTCCGGCATg  <  5:251756/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATACGGGCCTCTGCGGGTGAAAAAAGCACTGTCTCGGAGTAACTAAAGCATCCGGTACGGCGCTCTGCACGGCAATGCAGGAAACGGCATGGAGGCGATTGAAAAACGTCATGGCTGTTCGCTTGTAGCGGCCTTTTCGC‑GGCTAAAACCGCGAAGAGGCCGGTACTGCGCATCAAAGCTTCGGCATCTGCCCGATCCGCGCCACCATTTCGCTCACCACCTGCAAGTCCAGCATGAACTGCTCCACGGTCTTGAACTCGTTGTCGTTGTGCCCGGTGTACTTCACCTCCGGCATG  >  NC_002947/253214‑253509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑